Occurrence and genetic determination of Giardia in dogs from South Eastern Europe:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen, Lahn
VVB Laufersweiler Verlag
2015
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Edition Scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 111 S. Ill., graph. Darst., Kt. |
| ISBN: | 9783835963504 3835963503 |
Internformat
MARC
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| 245 | 1 | 0 | |a Occurrence and genetic determination of Giardia in dogs from South Eastern Europe |c von Marie Franziska Sommer |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Gießen, Lahn |b VVB Laufersweiler Verlag |c 2015 | |
| 300 | |a X, 111 S. |b Ill., graph. Darst., Kt. | ||
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| 650 | 7 | |a dogs |2 cabt | |
| 650 | 7 | |a giardia |2 cabt | |
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| 650 | 7 | |a genetic |2 cabt | |
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Datensatz im Suchindex
| _version_ | 1804175412359593984 |
|---|---|
| adam_text | TABLE OF CONTENT
TABLE OF CONTENT
ABBREVIATIONS X
I. INTRODUCTION 1
II. LITERATURE REVIEW 3
1. GIARDIA DUODENALIS 3
1.1. TAXONOMY AND ASSEMBLAGES 3
1.2. MORPHOLOGY 7
1.3. LIFE CYCLE 7
1.4. PATHOGENESIS AND CLINICAL SYMPTOMS 8
1.5. EPIDEMIOLOGY 9
1.6. ZOONOTIC POTENTIAL 10
1.7. DIAGNOSTICS 12
1.8. TREATMENT OF GIARDIA INFECTIONS 14
2. G. DUODENALIS IN SOUTH EASTERN EUROPE 15
2.1. ALBANIA 15
2.2. BULGARIA 16
2.3. CROATIA 16
2.4. HUNGARY 17
2.5. MACEDONIA 17
2.6. ROMANIA 17
2.7. SERBIA 18
III. MATERIALS AND METHODS 21
1. SAMPLE ORIGIN 21
2. SCREENING FOR GIARDIA POSITIVE SAMPLES 22
2.1. ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) 22
3. SCREENING FOR GIARDIA CYSTS 23
3.1. SCREENING WITH IMMUNOFLUORESCENCE ASSAY (IFA) 23
3.2. SCREENING WITH MERTHIOLATE IODINE FORMALIN CONCENTRATION (MIFC) 24
4. DNA EXTRACTION 25
5. DNA PURIFICATION ....................... ........ 25
VII
HTTP://D-NB.INFO/1076024084
TABLE OF CONTENL
6. QUALITY CONTROL OF EXTRACTION AND QUANTISATION OF DNA 25
7. POLYMERASE CHAIN REACTION FOR DETECTION OF GIARDIA DNA 26
7.1. NESTED PCR FOR THE DETECTION OF THE SSU RRNA GENE 27
7.2. NESTED PCR FOR THE DETECTION OF THE ITS 1 -5.8S-ITS2 REGION 28
7.3. NESTED PCR FOR THE DETECTION OF THE BETA GIARDIN GENE 29
7.4. NESTED PCR FOR THE DETECTION OF THE GLUTAMATE DEHYDROGENASE GENE
30
7.5. NESTED PCR FOR THE DETECTION OF THE TRIOSEPHOSPHATE ISOMERASE GENE
31
8. VISUALISATION OF PCR PRODUCTS 32
8.1. AGAROSE GEL ELECTROPHORESIS 32
8.2. CAPILLARY ELECTROPHORESIS 33
9. DNA PURIFICATION 33
10. SEQUENCING AND SEQUENCE ANALYSIS: DETERMINATION OF ASSEMBLAGES 33
11. TRANSLATION OF NUCLEOTIDE SEQUENCES INTO AMINO ACIDS 33
12. STATISTICAL ANALYSIS 34
IV. RESULTS 35
1. PUBLICATION 35
2. FURTHER RESULTS 58
V. DISCUSSION 61
VI. CONCLUSION 69
VII. SUMMARY 70
VIII. ZUSAMMENFASSUNG 72
IX. REFERENCES 74
X. FIGURES 93
XI. TABLES 94
XII. ANNEX 95
1. GLOBAL PREVALENCE DATA OF G. DUODENA/IS 95
2. FREQUENTLY USED GENES FOR MOLECULAR TYPING OF G. DUODENALIS 98
3. NOMENCLATURE FOR INCOMPLETELY SPECIFIED BASES IN NUCLEIC ACID
SEQUENCES.. 99
VIII
TABLE OF CONTENT
4. SEQUENCE COMPARISON WITH GENBANK 100
5. COMBINED GENOTYPING RESULTS 100
6. EQUIPMENT 100
7. KITS 101
8. CHEMICALS 101
9. NUCLEOTIDES AND PRIMERS 102
10. BUFFER AND SOLUTION FOR AGAROSE GEL ELECTROPHORESIS 102
11. SEQUENCING DATA 102
11.1. SSU RRNA SEQUENCE COMPARISON OF G. DUODENAL IS 102
11.1.1. ALIGNMENT OF NUCLEOTIDE SEQUENCES 102
11.2. ITS
1 -5.8S-ITS2 SEQUENCE COMPARISON OF G. DUODENALIS 103
11.2.1. ALIGNMENT OF NUCLEOTIDE SEQUENCES 103
11.3. BETA GIARDIN SEQUENCE COMPARISON OF G. DUODENALIS 104
11.3.1. ALIGNMENT OF NUCLEOTIDE SEQUENCES 104
11.3.2. ALIGNMENT OF AMINO ACIDS 106
11.4. GLUTAMATE DEHYDROGENASE SEQUENCE COMPARISON OF G. DUODENALIS 106
11.4.1. ALIGNMENT OF NUCLEOTIDE SEQUENCES 106
11.4.2. ALIGNMENT OF AMINO ACIDS 107
11.5. TRIOSEPHOSPHATE ISOMERASE SEQUENCE COMPARISON OF G. DUODENALIS 107
11.5.1. ALIGNMENT OF NUCLEOTIDE SEQUENCES 107
11.5.2. ALIGNMENT OF AMINO ACIDS 108
XIII. ACKNOWLEDGEMENTS 109
IX
|
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| spelling | Sommer, Marie Franziska Verfasser aut Occurrence and genetic determination of Giardia in dogs from South Eastern Europe von Marie Franziska Sommer 1. Aufl. Gießen, Lahn VVB Laufersweiler Verlag 2015 X, 111 S. Ill., graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Edition Scientifique Zugl.: München, Univ., Diss., 2015 dogs cabt giardia cabt europe cabt genetic cabt occurrence cabt Doktorarbeit Uni Wissenschaft (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028470041&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sommer, Marie Franziska Occurrence and genetic determination of Giardia in dogs from South Eastern Europe dogs cabt giardia cabt europe cabt genetic cabt occurrence cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe |
| title_auth | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe |
| title_exact_search | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe |
| title_full | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe von Marie Franziska Sommer |
| title_fullStr | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe von Marie Franziska Sommer |
| title_full_unstemmed | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe von Marie Franziska Sommer |
| title_short | Occurrence and genetic determination of Giardia in dogs from South Eastern Europe |
| title_sort | occurrence and genetic determination of giardia in dogs from south eastern europe |
| topic | dogs cabt giardia cabt europe cabt genetic cabt occurrence cabt |
| topic_facet | dogs giardia europe genetic occurrence Hochschulschrift |
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