Molecular biology techniques: an intensive laboratory course
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
San Diego
Academic Press
1999
|
| Schlagworte: | |
| Online-Zugang: | FAW01 FAW02 Volltext |
| Beschreibung: | Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- - Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- - Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter |
| Beschreibung: | 1 Online-Ressource (x, 234 pages) |
| ISBN: | 0080536824 0125839901 9780080536828 9780125839907 |
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| 500 | |a Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- | ||
| 500 | |a - Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- | ||
| 500 | |a - Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index | ||
| 500 | |a This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter | ||
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Datensatz im Suchindex
| _version_ | 1804175411368689664 |
|---|---|
| any_adam_object | |
| author | Ream, Walt |
| author_facet | Ream, Walt |
| author_role | aut |
| author_sort | Ream, Walt |
| author_variant | w r wr |
| building | Verbundindex |
| bvnumber | BV043044970 |
| collection | ZDB-4-EBA |
| ctrlnum | (OCoLC)173520343 (DE-599)BVBBV043044970 |
| dewey-full | 572.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
| dewey-sort | 3572.8 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Electronic eBook |
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| id | DE-604.BV043044970 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T07:15:52Z |
| institution | BVB |
| isbn | 0080536824 0125839901 9780080536828 9780125839907 |
| language | English |
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| physical | 1 Online-Ressource (x, 234 pages) |
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| spelling | Ream, Walt Verfasser aut Molecular biology techniques an intensive laboratory course Walt Ream and Katharine G. Field San Diego Academic Press 1999 1 Online-Ressource (x, 234 pages) txt rdacontent c rdamedia cr rdacarrier Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- - Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- - Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter Moleküler biyoloji / Laboratuvar el kitapları Biologie moléculaire / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh Molecular biology / Technique fast Molecular Biology / Laboratory Manuals Molecular biology Technique Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s 1\p DE-604 Field, Katharine G. Sonstige oth http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203136 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Ream, Walt Molecular biology techniques an intensive laboratory course Moleküler biyoloji / Laboratuvar el kitapları Biologie moléculaire / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh Molecular biology / Technique fast Molecular Biology / Laboratory Manuals Molecular biology Technique Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 |
| title | Molecular biology techniques an intensive laboratory course |
| title_auth | Molecular biology techniques an intensive laboratory course |
| title_exact_search | Molecular biology techniques an intensive laboratory course |
| title_full | Molecular biology techniques an intensive laboratory course Walt Ream and Katharine G. Field |
| title_fullStr | Molecular biology techniques an intensive laboratory course Walt Ream and Katharine G. Field |
| title_full_unstemmed | Molecular biology techniques an intensive laboratory course Walt Ream and Katharine G. Field |
| title_short | Molecular biology techniques |
| title_sort | molecular biology techniques an intensive laboratory course |
| title_sub | an intensive laboratory course |
| topic | Moleküler biyoloji / Laboratuvar el kitapları Biologie moléculaire / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh Molecular biology / Technique fast Molecular Biology / Laboratory Manuals Molecular biology Technique Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Moleküler biyoloji / Laboratuvar el kitapları Biologie moléculaire / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology Molecular biology / Technique Molecular Biology / Laboratory Manuals Molecular biology Technique Molekularbiologie Methode |
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