PCR applications: protocols for functional genomics
Gespeichert in:
Format: | Elektronisch E-Book |
---|---|
Sprache: | English |
Veröffentlicht: |
San Diego
Academic Press
©1999
|
Schlagworte: | |
Online-Zugang: | FAW01 FAW02 Volltext |
Beschreibung: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references and index pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR. Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards |
Beschreibung: | 1 Online-Ressource (xviii, 566 pages, [3] pages of plates) |
ISBN: | 0080919634 0123721857 9780080919638 9780123721853 |
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500 | |a Includes bibliographical references and index | ||
500 | |a pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity | ||
500 | |a Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR. | ||
500 | |a Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research | ||
500 | |a Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome | ||
500 | |a Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning | ||
500 | |a PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards | ||
650 | 4 | |a Genomes | |
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650 | 4 | |a Réaction en chaîne de la polymérase | |
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650 | 4 | |a Polymerase Chain Reaction / methods | |
650 | 4 | |a Genetic Engineering | |
650 | 4 | |a Polymerase chain reaction | |
650 | 4 | |a Gene amplification | |
650 | 4 | |a Polymerase chain reaction |v Laboratory manuals | |
650 | 4 | |a Gene amplification |v Laboratory manuals | |
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Datensatz im Suchindex
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any_adam_object | |
building | Verbundindex |
bvnumber | BV043044224 |
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collection | ZDB-4-EBA |
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dewey-full | 572.8/6 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572.8/6 |
dewey-search | 572.8/6 |
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publisher | Academic Press |
record_format | marc |
spelling | PCR applications protocols for functional genomics edited by Michael A. Innis, David H. Gelfand, John J. Sninsky San Diego Academic Press ©1999 1 Online-Ressource (xviii, 566 pages, [3] pages of plates) txt rdacontent c rdamedia cr rdacarrier Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references and index pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR. Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards Genomes Polymerase chain reaction / Diagnostic use Réaction en chaîne de la polymérase Amplification génique Réaction en chaîne de la polymérase / Manuels de laboratoire Amplification génique / Manuels de laboratoire SCIENCE / Life Sciences / Genetics & Genomics bisacsh Polymerase kettingreactie gtt Genoom gtt Gene amplification fast Genomics fast Polymerase chain reaction fast Polymerase Chain Reaction / methods Genetic Engineering Polymerase chain reaction Gene amplification Polymerase chain reaction Laboratory manuals Gene amplification Laboratory manuals Genomics Laboratory manuals Methode (DE-588)4038971-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Polymerase-Kettenreaktion (DE-588)4256726-9 s Methode (DE-588)4038971-6 s 2\p DE-604 Sninsky, John J. Sonstige oth Innis, Michael A. Sonstige oth Gelfand, David H. Sonstige oth Erscheint auch als Druck-Ausgabe, Paperback 0-12-372186-5 Erscheint auch als Druck-Ausgabe, Paperback 978-0-12-372186-0 http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=239295 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | PCR applications protocols for functional genomics Genomes Polymerase chain reaction / Diagnostic use Réaction en chaîne de la polymérase Amplification génique Réaction en chaîne de la polymérase / Manuels de laboratoire Amplification génique / Manuels de laboratoire SCIENCE / Life Sciences / Genetics & Genomics bisacsh Polymerase kettingreactie gtt Genoom gtt Gene amplification fast Genomics fast Polymerase chain reaction fast Polymerase Chain Reaction / methods Genetic Engineering Polymerase chain reaction Gene amplification Polymerase chain reaction Laboratory manuals Gene amplification Laboratory manuals Genomics Laboratory manuals Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
subject_GND | (DE-588)4038971-6 (DE-588)4256726-9 (DE-588)4143413-4 |
title | PCR applications protocols for functional genomics |
title_auth | PCR applications protocols for functional genomics |
title_exact_search | PCR applications protocols for functional genomics |
title_full | PCR applications protocols for functional genomics edited by Michael A. Innis, David H. Gelfand, John J. Sninsky |
title_fullStr | PCR applications protocols for functional genomics edited by Michael A. Innis, David H. Gelfand, John J. Sninsky |
title_full_unstemmed | PCR applications protocols for functional genomics edited by Michael A. Innis, David H. Gelfand, John J. Sninsky |
title_short | PCR applications |
title_sort | pcr applications protocols for functional genomics |
title_sub | protocols for functional genomics |
topic | Genomes Polymerase chain reaction / Diagnostic use Réaction en chaîne de la polymérase Amplification génique Réaction en chaîne de la polymérase / Manuels de laboratoire Amplification génique / Manuels de laboratoire SCIENCE / Life Sciences / Genetics & Genomics bisacsh Polymerase kettingreactie gtt Genoom gtt Gene amplification fast Genomics fast Polymerase chain reaction fast Polymerase Chain Reaction / methods Genetic Engineering Polymerase chain reaction Gene amplification Polymerase chain reaction Laboratory manuals Gene amplification Laboratory manuals Genomics Laboratory manuals Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
topic_facet | Genomes Polymerase chain reaction / Diagnostic use Réaction en chaîne de la polymérase Amplification génique Réaction en chaîne de la polymérase / Manuels de laboratoire Amplification génique / Manuels de laboratoire SCIENCE / Life Sciences / Genetics & Genomics Polymerase kettingreactie Genoom Gene amplification Genomics Polymerase chain reaction Polymerase Chain Reaction / methods Genetic Engineering Polymerase chain reaction Laboratory manuals Gene amplification Laboratory manuals Genomics Laboratory manuals Methode Polymerase-Kettenreaktion Aufsatzsammlung |
url | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=239295 |
work_keys_str_mv | AT sninskyjohnj pcrapplicationsprotocolsforfunctionalgenomics AT innismichaela pcrapplicationsprotocolsforfunctionalgenomics AT gelfanddavidh pcrapplicationsprotocolsforfunctionalgenomics |