The genetic manipulation of staphylococci: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Humana Press
New York, NY [u.a.]
2016
|
| Schriftenreihe: | Methods in molecular biology
1373 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 186 S. Ill., graph. Darst. |
| ISBN: | 9781493931576 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV042920853 | ||
| 003 | DE-604 | ||
| 005 | 20151120 | ||
| 007 | t | ||
| 008 | 151012s2016 ad|| |||| 00||| eng d | ||
| 020 | |a 9781493931576 |9 978-1-4939-3157-6 | ||
| 035 | |a (OCoLC)932582804 | ||
| 035 | |a (DE-599)BVBBV042920853 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-11 |a DE-20 | ||
| 084 | |a WG 4100 |0 (DE-625)148561: |2 rvk | ||
| 245 | 1 | 0 | |a The genetic manipulation of staphylococci |b methods and protocols |c ed. by Jeffrey L. Bose |
| 264 | 1 | |a Humana Press |b New York, NY [u.a.] |c 2016 | |
| 300 | |a X, 186 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 1373 | |
| 490 | 0 | |a Springer protocols | |
| 700 | 1 | |a Bose, Jeffrey L. |4 edt | |
| 830 | 0 | |a Methods in molecular biology |v 1373 |w (DE-604)BV035362695 |9 1373 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028348321&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-028348321 | ||
Datensatz im Suchindex
| _version_ | 1804175222864084992 |
|---|---|
| adam_text | Titel: The genetic manipulation of staphylococci
Autor: Bose, Jeffrey L
Jahr: 2016
Contents
Preface.................................................................... v
Contributors............................................................... ix
Understanding Staphylococcal Nomenclature................................. 1
Christina N. Krute and Jeffrey L. Bose
Restriction-Modification Systems as a Barrier for Genetic Manipulation
of Staphylococcus aureus................................................... 9
Marat R. Sadykov
Complementation Plasmids, Inducible Gene-Expression Systems,
and Reporters for Staphylococci............................................. 25
Ralph Bertram
De Novo Assembly of Plasmids Using Yeast Recombinational Cloning........... 33
Ameya A. Mashruwala and Jeffrey M. Boyd
Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products........... 43
Justin A. Thornton
Method for Preparation and Electroporation of S. aureus
and S. epidermidis......................................................... 51
Melinda R. Grosser and Anthony R. Richardson
Rapid Isolation of DNA from Staphylococcus................................. 59
Kelsey L. Krausz and Jeffrey L. Bose
Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method..... 63
Kelsey L. Krausz and Jeffrey L. Bose
Bacteriophage Transduction in Staphylococcus aureus.......................... 69
Michael E. Olson
Batch Transduction of Transposon Mutant Libraries for Rapid
Phenotype Screening in Staphylococcus...................................... 75
Katherine L. Maliszewski
Conjugative Transfer in Staphylococcus aureus................................ 83
Cortney R. Halsey and Paul D. Fey
Allelic Exchange........................................................... 89
McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles
Creation of Staphylococcal Mutant Libraries Using
Transposon Tn917......................................................... 97
Kelly C. Rice
Generation of a Transposon Mutant Library in Staphylococcus aureus
and Staphylococcus epidermidis Using bursa aurealis........................... 103
Vijaya Kumar Tajjala, Todd J. Widhelm, Jennifer L. Endres,
Paul D. Fey, and Kenneth W. Bayles
Chemical and UV Mutagenesis.............................................. Ill
Jeffrey L. Bose
Pulse Field Gel Electrophoresis.............................................. 117
Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler Jr.
vii
viii Contents
RNA-Sequencing of Staphylococcus aureus Messenger RNA.................... 131
Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw
Quantitative Real-Time PCR (qPCR) Workflow for Analyzing
Staphylococcus aureus Gene Expression...................................... 143
April M. Lewis and Kelly C. Rice
Electrophoretic Mobility Shift Assays......................................... 155
Sarah E. Rowe and James P. O Gara
Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in
Staphylococcus............................................................ 169
Eric Miller
Index..................................................................... 185
|
| any_adam_object | 1 |
| author2 | Bose, Jeffrey L. |
| author2_role | edt |
| author2_variant | j l b jl jlb |
| author_facet | Bose, Jeffrey L. |
| building | Verbundindex |
| bvnumber | BV042920853 |
| classification_rvk | WG 4100 |
| ctrlnum | (OCoLC)932582804 (DE-599)BVBBV042920853 |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01219nam a2200313 cb4500</leader><controlfield tag="001">BV042920853</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20151120 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">151012s2016 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493931576</subfield><subfield code="9">978-1-4939-3157-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)932582804</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV042920853</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-11</subfield><subfield code="a">DE-20</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 4100</subfield><subfield code="0">(DE-625)148561:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The genetic manipulation of staphylococci</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">ed. by Jeffrey L. Bose</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Humana Press</subfield><subfield code="b">New York, NY [u.a.]</subfield><subfield code="c">2016</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">X, 186 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">1373</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Springer protocols</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bose, Jeffrey L.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">1373</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">1373</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028348321&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028348321</subfield></datafield></record></collection> |
| id | DE-604.BV042920853 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:12:52Z |
| institution | BVB |
| isbn | 9781493931576 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028348321 |
| oclc_num | 932582804 |
| open_access_boolean | |
| owner | DE-11 DE-20 |
| owner_facet | DE-11 DE-20 |
| physical | X, 186 S. Ill., graph. Darst. |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| publisher | New York, NY [u.a.] |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | The genetic manipulation of staphylococci methods and protocols ed. by Jeffrey L. Bose Humana Press New York, NY [u.a.] 2016 X, 186 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1373 Springer protocols Bose, Jeffrey L. edt Methods in molecular biology 1373 (DE-604)BV035362695 1373 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028348321&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The genetic manipulation of staphylococci methods and protocols Methods in molecular biology |
| title | The genetic manipulation of staphylococci methods and protocols |
| title_auth | The genetic manipulation of staphylococci methods and protocols |
| title_exact_search | The genetic manipulation of staphylococci methods and protocols |
| title_full | The genetic manipulation of staphylococci methods and protocols ed. by Jeffrey L. Bose |
| title_fullStr | The genetic manipulation of staphylococci methods and protocols ed. by Jeffrey L. Bose |
| title_full_unstemmed | The genetic manipulation of staphylococci methods and protocols ed. by Jeffrey L. Bose |
| title_short | The genetic manipulation of staphylococci |
| title_sort | the genetic manipulation of staphylococci methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028348321&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT bosejeffreyl thegeneticmanipulationofstaphylococcimethodsandprotocols |