Hybrid solid-state nanopores for stochastic single-molecule detection:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Garching
Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München
2015
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Selected topics of semiconductor physics and technology
188 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 109 S. Ill., graph. Darst. |
| ISBN: | 9783941650886 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV042872689 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 150916s2015 ad|| m||| 00||| eng d | ||
| 020 | |a 9783941650886 |9 978-3-941650-88-6 | ||
| 035 | |a (OCoLC)926990750 | ||
| 035 | |a (DE-599)BVBBV042872689 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91 |a DE-12 |a DE-83 | ||
| 082 | 0 | |a 539.6 |2 22//ger | |
| 084 | |a PHY 685d |2 stub | ||
| 084 | |a TEC 030d |2 stub | ||
| 084 | |a ELT 300d |2 stub | ||
| 100 | 1 | |a Wei, Ruoshan |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Hybrid solid-state nanopores for stochastic single-molecule detection |c Ruoshan Wei |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Garching |b Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München |c 2015 | |
| 300 | |a VI, 109 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Selected topics of semiconductor physics and technology |v 188 | |
| 502 | |a Zugl.: München, Techn. Univ., Diss., 2015 | ||
| 650 | 0 | 7 | |a Nanoporöser Stoff |0 (DE-588)4800345-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Halbleiter |0 (DE-588)4022993-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Einzelmolekülmikroskopie |0 (DE-588)7669710-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biomolekül |0 (DE-588)4135124-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Einzelmolekülmikroskopie |0 (DE-588)7669710-1 |D s |
| 689 | 0 | 1 | |a Nanoporöser Stoff |0 (DE-588)4800345-1 |D s |
| 689 | 0 | 2 | |a Biomolekül |0 (DE-588)4135124-1 |D s |
| 689 | 0 | 3 | |a Halbleiter |0 (DE-588)4022993-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 830 | 0 | |a Selected topics of semiconductor physics and technology |v 188 |w (DE-604)BV011499438 |9 188 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028301772&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-028301772 | ||
Datensatz im Suchindex
| _version_ | 1804175155329499136 |
|---|---|
| adam_text | CONTENTS
ABSTRACT I
ZUSAMMENFASSUNG II
PUBLICATIONS W
CONTENTS IV
I| INTRODUCTION 1
21 BASICS 5
2.1 FABRICATION 5
2.1.1 TEM DRILLING 6
2.1.2 ELECTRON-BEAM LITHOGRAPHY 6
2.1.3 METALLIZATION 8
2.2 ELECTRICAL CHARACTERIZATION 10
2.2.1 INSTRUMENTATION AND SETUP 11
2.2.2 NANOPORE CONDUCTANCE 11
2.2.3 FINITE ELEMENT METHOD SIMULATION OF NANOPORES 13
2.2.4 ELECTRICAL NOISE OF NANOPORES 14
2.3 SINGLE MOLECULE DETECTION 15
2.3.1 CURRENT BLOCKADE 15
2.3.2 TRANSLOCATION TIME 16
2.3.3 EVENT RATE 18
3| THE INFLUENCE OF HYDROPHOBIC INTERACTIONS ON PROTEIN TRANSLOCATIONS
21
3.1 INTRODUCTION 21
3.2 EXPERIMENTAL INDICATIONS -
GATHERING THE EVIDENCE 22
3.3 PROPOSED THEORIES 25
3.3.1 LIMITED TEMPORAL RESOLUTION 25
3.3.2 HYDRODYNAMIC DRAG IN CONFINEMENT 27
3.3.3 ELECTROOSMOSIS 28
3.3.4 PROTEIN-PORE INTERACTION 29
3.4 PROTEIN-PORE INTERACTIONS 29
3.4.1 FIRST-PASSAGE TIME MODEL OF PROTEIN-PORE INTERACTIONS 29
3.4.2 BROWNIAN FEM SIMULATION 33
3.5 NATURE OF PROTEIN-PORE INTERACTIONS 35
3.5.1 ELECTROSTATIC INTERACTION 36
3.5.2 HYDROPHOBIC INTERACTIONS 37
3.5.3 HSPGO AS A MODEL SYSTEM FOR THE MODULATION OF PROTEIN
HYDROPHOBICITY 37
3.5.4 HYDROPHOBIC PATCH ANALYSIS 39
IV
HTTP://D-NB.INFO/1076009107
3.6 DISCUSSION 43
3.6.1 SIN SURFACE HYDROPHOBICITY 43
3.6.2 CONFORMATION CHANGE 43
3.7 CONCLUSION 44
4| STOCHASTIC SENSING WITH CHEMICALLY MODIFIED NANOPORES 47
4.1 INTRODUCTION 47
4.2 CHEMICAL MODIFICATION OF METALIZED NANOPORES 48
4.2.1 SAM ASSEMBLY 48
4.2.2 MIXED MONOLAYERS 50
4.3 NANOPORES AS STOCHASTIC SENSORS 51
4.3.1 STOCHASTIC SENSING 51
4.3.2 QUANTIFICATION OF DISSOCIATION RATES 54
4.3.3 RECEPTOR MULTIVALENCY 56
4.4 SINGLE-MOLECULE SANDWICH ASSAY 57
4.5 DISCUSSION 60
4.5.1 SAM FORMATION 60
4.5.2 NUMBER OF RECEPTORS 61
4.5.3 STATISTICS OF SINGLE-MOLECULE MEASUREMENT 62
4.6 CONCLUSION 63
5| DNA ORIGAMI GATEKEEPERS FOR SOUD-STATE NANOPORES 65
5.1 INTRODUCTION 65
5.2 DNA NANOPLATE CAPTURE AND
CHARACTERIZATION 66
5.2.1 NANOPLATE CAPTURE ONTO SIN NANOPORES 66
5.2.2 CONDUCTANCE OF THE NANOPLATE-ON-NANOPORE HYBRID 68
5.3 SINGLE-MOLECULE DETECTION 70
5.3.1 NANOPLATE AS SIZE FILTER 70
5.3.2 DNA TRANSLOCATION THOUGH NANOPLATE 71
5.4 NANOPLATES AS SEQUENCE-SPECIFIC GATEKEEPERS 73
5.4.1 SHORT OLIGONUCLEOTIDES 74
5.4.2 BACTERIOPHAGE GENOME 75
5.5 DISCUSSION 76
5.5.1 NOISE ANALYSIS 76
5.5.2 STATISTICS OF NANOPLATE CONDUCTANCE 77
5.5.3 NANOPLATES AT LOW SALT 78
5.6 CONCLUSION 79
6| CONCLUSION AND OUTLOOK 81
A| APPENDIX 85
A.I NANOPORE FABRICATION AND C
HARACTERIZATION 85
A.2 INFLUENCE OF HYDROPHOBIC PROTEIN-PORE INTERACTIONS 88
A. 3 STOCHASTIC SENSING WITH RECEPTOR-MODIFIED NANOPORES 91
V
A.4 DNAORIGAMI GATEKEEPERS 94
B| BIBLIOGRAPHY 97
C| ACKNOWLEDGEMENTS M
VI
|
| any_adam_object | 1 |
| author | Wei, Ruoshan |
| author_facet | Wei, Ruoshan |
| author_role | aut |
| author_sort | Wei, Ruoshan |
| author_variant | r w rw |
| building | Verbundindex |
| bvnumber | BV042872689 |
| classification_tum | PHY 685d TEC 030d ELT 300d |
| ctrlnum | (OCoLC)926990750 (DE-599)BVBBV042872689 |
| dewey-full | 539.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 539 - Modern physics |
| dewey-raw | 539.6 |
| dewey-search | 539.6 |
| dewey-sort | 3539.6 |
| dewey-tens | 530 - Physics |
| discipline | Physik Technik Elektrotechnik |
| edition | 1. Aufl. |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02028nam a2200481 cb4500</leader><controlfield tag="001">BV042872689</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">150916s2015 ad|| m||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783941650886</subfield><subfield code="9">978-3-941650-88-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)926990750</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV042872689</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-83</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">539.6</subfield><subfield code="2">22//ger</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">PHY 685d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">TEC 030d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">ELT 300d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wei, Ruoshan</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Hybrid solid-state nanopores for stochastic single-molecule detection</subfield><subfield code="c">Ruoshan Wei</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. Aufl.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Garching</subfield><subfield code="b">Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München</subfield><subfield code="c">2015</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">VI, 109 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Selected topics of semiconductor physics and technology</subfield><subfield code="v">188</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Zugl.: München, Techn. Univ., Diss., 2015</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Nanoporöser Stoff</subfield><subfield code="0">(DE-588)4800345-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Halbleiter</subfield><subfield code="0">(DE-588)4022993-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Einzelmolekülmikroskopie</subfield><subfield code="0">(DE-588)7669710-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biomolekül</subfield><subfield code="0">(DE-588)4135124-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Einzelmolekülmikroskopie</subfield><subfield code="0">(DE-588)7669710-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Nanoporöser Stoff</subfield><subfield code="0">(DE-588)4800345-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Biomolekül</subfield><subfield code="0">(DE-588)4135124-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Halbleiter</subfield><subfield code="0">(DE-588)4022993-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Selected topics of semiconductor physics and technology</subfield><subfield code="v">188</subfield><subfield code="w">(DE-604)BV011499438</subfield><subfield code="9">188</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028301772&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028301772</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV042872689 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:11:47Z |
| institution | BVB |
| isbn | 9783941650886 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028301772 |
| oclc_num | 926990750 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-12 DE-83 |
| owner_facet | DE-91 DE-BY-TUM DE-12 DE-83 |
| physical | VI, 109 S. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München |
| record_format | marc |
| series | Selected topics of semiconductor physics and technology |
| series2 | Selected topics of semiconductor physics and technology |
| spelling | Wei, Ruoshan Verfasser aut Hybrid solid-state nanopores for stochastic single-molecule detection Ruoshan Wei 1. Aufl. Garching Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München 2015 VI, 109 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Selected topics of semiconductor physics and technology 188 Zugl.: München, Techn. Univ., Diss., 2015 Nanoporöser Stoff (DE-588)4800345-1 gnd rswk-swf Halbleiter (DE-588)4022993-2 gnd rswk-swf Einzelmolekülmikroskopie (DE-588)7669710-1 gnd rswk-swf Biomolekül (DE-588)4135124-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Einzelmolekülmikroskopie (DE-588)7669710-1 s Nanoporöser Stoff (DE-588)4800345-1 s Biomolekül (DE-588)4135124-1 s Halbleiter (DE-588)4022993-2 s DE-604 Selected topics of semiconductor physics and technology 188 (DE-604)BV011499438 188 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028301772&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wei, Ruoshan Hybrid solid-state nanopores for stochastic single-molecule detection Selected topics of semiconductor physics and technology Nanoporöser Stoff (DE-588)4800345-1 gnd Halbleiter (DE-588)4022993-2 gnd Einzelmolekülmikroskopie (DE-588)7669710-1 gnd Biomolekül (DE-588)4135124-1 gnd |
| subject_GND | (DE-588)4800345-1 (DE-588)4022993-2 (DE-588)7669710-1 (DE-588)4135124-1 (DE-588)4113937-9 |
| title | Hybrid solid-state nanopores for stochastic single-molecule detection |
| title_auth | Hybrid solid-state nanopores for stochastic single-molecule detection |
| title_exact_search | Hybrid solid-state nanopores for stochastic single-molecule detection |
| title_full | Hybrid solid-state nanopores for stochastic single-molecule detection Ruoshan Wei |
| title_fullStr | Hybrid solid-state nanopores for stochastic single-molecule detection Ruoshan Wei |
| title_full_unstemmed | Hybrid solid-state nanopores for stochastic single-molecule detection Ruoshan Wei |
| title_short | Hybrid solid-state nanopores for stochastic single-molecule detection |
| title_sort | hybrid solid state nanopores for stochastic single molecule detection |
| topic | Nanoporöser Stoff (DE-588)4800345-1 gnd Halbleiter (DE-588)4022993-2 gnd Einzelmolekülmikroskopie (DE-588)7669710-1 gnd Biomolekül (DE-588)4135124-1 gnd |
| topic_facet | Nanoporöser Stoff Halbleiter Einzelmolekülmikroskopie Biomolekül Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028301772&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011499438 |
| work_keys_str_mv | AT weiruoshan hybridsolidstatenanoporesforstochasticsinglemoleculedetection |