Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
VVB Laufersweiler
2014
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 97 S. Ill., graph. Darst. 21 cm, 209 g |
| ISBN: | 9783835961869 |
Internformat
MARC
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| 100 | 1 | |a Dau, Thérèse Thuy Dung |d 1983- |e Verfasser |0 (DE-588)107080780X |4 aut | |
| 245 | 1 | 0 | |a Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |c Thérèse Dau |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Gießen |b VVB Laufersweiler |c 2014 | |
| 300 | |a 97 S. |b Ill., graph. Darst. |c 21 cm, 209 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Zugl.: München, Univ., Diss., 2014 | ||
| 650 | 0 | 7 | |a Leukocyte Elastase |0 (DE-588)1071079964 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Chemokine |0 (DE-588)4429989-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Entzündung |0 (DE-588)4014975-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Myeloblastin |0 (DE-588)107107959X |2 gnd |9 rswk-swf |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-028197229 | ||
Datensatz im Suchindex
| _version_ | 1804174989191020544 |
|---|---|
| adam_text | 1 SUMMARY -1-
2 INTRODUCTION -4-
2.1 NEUTROPHIL GRANULOCYTES - 4 -
2.2 NEUTROPHIL SERINE PROTEASES - 5 -
2.2.1 REGULATORY FUNCTIONS OF NSPS - 6 -
2.2.2 PATHOPHYSIOLOGICAL FUNCTIONS OF NSPS - 7 -
2.2.3 CHEMOKINES AS SUBSTRATES OF
NSPS - 8 -
2.3 NEUTROPHIL ELASTASE - 9 -
2.3.1 NE IN LUNG
DISEASES -10 -
2.3.2 REGULATION OF NE ACTIVITY -11 -
3 MATERIAL AND METHODS -15 -
3.1 NUCLEIC ACID METHODS -15 -
3.1.1 POLYMERASE-CHAIN REACTION (PCR) -15 -
3.1.2 RESTRICTION DIGEST -15 -
3.1.3 DNA GEL ELECTROPHORESIS -15-
3.1.4 DEPHOSPHORYLATION -16 -
3.1.5 LIGATION -16-
3.1.6 TRANSFORMATION -16-
3.1.7 COMPETENT CELLS -17-
3.1.8 DNA PURIFICATION -17 -
3.2 PROTEIN METHODS -17 -
3.2.1 ANTIBODY LIST -18 -
3.2.2 AFFINITY CHROMATOGRAPHY -18 -
3.2.3
ACTIVATION OF
NSPS
BY ENTEROKINASE CLEAVAGE
-19-
3.2.4 SPECTROPHOTOMETRY QUANTIFICATION OF PROTEINS -19 -
3.2.5 QUANTIFICATION OF PROTEINS WITH BICINCHONINIC ACID (BCA) ASSAY -
20 -
3.2.6 ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) - 20 -
3.2.7 DISCONTINUOUS GEL ELECTROPHORESIS - 20 -
-8-
HTTP://D-NB.INFO/1053833652
3.2.8 STAINING OF PROTEIN GELS -22-
3.2.9 COOMASS1E BLUE STAINING - 22 -
3.2.10 SILVER STAINING - 23 -
3.2.11 WESTERN BLOT - 23 -
3.2.12 ACTIVITY ASSAY -25-
3.2.13 IMMUNOPRECIPITATION OF MIP-2 - 26 -
3.2.14 PREPARATION OF PMNS LYSATE - 26 -
3.3 KINETIC METHODS - 27 -
3.3.1 BURST TITRATION -27-
3.3.2 TITRATION -27-
3.3.3 KM AND KCAT -27-
3.3.4 COMPETITIVE INHIBITION - 28 -
3.3.5 IRREVERSIBLE INHIBITION - 29 -
3.4 CELL BIOLOGICAL METHODS - 31 -
3.4.1 CELLCULTURE -31-
3.4.2 TRANSFECTION - 3
1 -
3.4.3 ISOLATION OF GRANULOCYTES - 32 -
3.4.4 IDENTIFICATION OF SC- AND TC-NE IN BIOLOGICAL SAMPLES - 32 -
3.4.5 CHEMOTAXIS ASSAY - 33 -
3.4.6 FLOW CYTOMETRY -34-
4 RESULTS -35-
4.1
NSP REGULATE INFLAMMATION
BY
PROCESSING OF CHEMOKINES
- 35 -
4.1.1 N-TERMINAL PROCESSING OF MIP-2 BY NE AND PR3 IN-VITRO - 35 -
4.1.2 CHEMOTACTIC PROPERTIES OF MIP-2 - 37 -
4.1.3 FURTHER MIP-2 VARIANTS -41-
4.1.4 OTHER CHEMOKINES AS SUBSTRATES -45
-
4.2 NE ESCAPES INHIBITION BY SELF-CLEAVAGE - 46 -
4.2.1 SELF-CLEAVAGE OF NE -46-
4.2.2 DOES NE INACTIVATE ITSELF VIA SELF-CLEAVAGE? - 48 -
4.2.3 RESISTANT MNE - 50 -
4.2.4 TITRATION OF SC-AND TC-MNE - 52 -
4.2.5 TESTING OF DIFFERENT SUBSTRATES - 54 -
-9-
4.2.6 INHIBITORS - 56 -
4.2.7 NATURAL OCCURRENCE OF TC-MNE -61-
5 DISCUSSION -63-
5.1 NSP REGULATE INFLAMMATION BY PROCESSING CHEMOK1NES - 63 -
5.2 NE ESCAPES INHIBITION BY SELF-CLEAVAGE - 66 -
5J CONCLUSION -73-
6 BIBLIOGRAPHY -75-
7 ABBREVIATIONS -83-
8 APPENDIX -85-
8.1 VECTOR MAP OF PTTS -85-
8.2 SEQUENCE OF EXPRESSED PROTEINS - 86 -
8.2.1 PTT5_NGS_MNE(WT)_ST.H6 -86-
8.2.2 PTT5_NGS_MNE-KG 187/8_ST.H6 - 87 -
8.2.3 PTT5_MIP-(5-73)_H6 -88-
8.2.4 PTT5_EK_M IP-2( 1 -73)_H6 -88-
8.2.5 PTT
5_M IP-2(5-7 3)_RUB
Y_H6 - 89 -
8.2.6 PTT5_ CHERRY_M
IP-2( 1 -7 3)_H6 - 90 -
8.2.7 PTT5_A1PI_342K -91 -
8.3 LIST OF CONSTRUCTS - 94 -
9 CURRICULUM VITAE -95-
10 PUBLICATIONS -97-
-10-
|
| any_adam_object | 1 |
| author | Dau, Thérèse Thuy Dung 1983- |
| author_GND | (DE-588)107080780X |
| author_facet | Dau, Thérèse Thuy Dung 1983- |
| author_role | aut |
| author_sort | Dau, Thérèse Thuy Dung 1983- |
| author_variant | t t d d ttd ttdd |
| building | Verbundindex |
| bvnumber | BV042766821 |
| ctrlnum | (OCoLC)920471718 (DE-599)DNB1053833652 |
| dewey-full | 616.0473 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.0473 |
| dewey-search | 616.0473 |
| dewey-sort | 3616.0473 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-07-10T07:09:09Z |
| institution | BVB |
| isbn | 9783835961869 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028197229 |
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| physical | 97 S. Ill., graph. Darst. 21 cm, 209 g |
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| spelling | Dau, Thérèse Thuy Dung 1983- Verfasser (DE-588)107080780X aut Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 Thérèse Dau 1. Aufl. Gießen VVB Laufersweiler 2014 97 S. Ill., graph. Darst. 21 cm, 209 g txt rdacontent n rdamedia nc rdacarrier Zugl.: München, Univ., Diss., 2014 Leukocyte Elastase (DE-588)1071079964 gnd rswk-swf Chemokine (DE-588)4429989-8 gnd rswk-swf Entzündung (DE-588)4014975-4 gnd rswk-swf Myeloblastin (DE-588)107107959X gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Entzündung (DE-588)4014975-4 s Myeloblastin (DE-588)107107959X s Leukocyte Elastase (DE-588)1071079964 s Chemokine (DE-588)4429989-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19- DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028197229&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dau, Thérèse Thuy Dung 1983- Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 Leukocyte Elastase (DE-588)1071079964 gnd Chemokine (DE-588)4429989-8 gnd Entzündung (DE-588)4014975-4 gnd Myeloblastin (DE-588)107107959X gnd |
| subject_GND | (DE-588)1071079964 (DE-588)4429989-8 (DE-588)4014975-4 (DE-588)107107959X (DE-588)4113937-9 |
| title | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |
| title_auth | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |
| title_exact_search | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |
| title_full | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 Thérèse Dau |
| title_fullStr | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 Thérèse Dau |
| title_full_unstemmed | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 Thérèse Dau |
| title_short | Proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |
| title_sort | proteolytic modification of elastase and chemokine activities by neutrophil elastase and proteinase 3 |
| topic | Leukocyte Elastase (DE-588)1071079964 gnd Chemokine (DE-588)4429989-8 gnd Entzündung (DE-588)4014975-4 gnd Myeloblastin (DE-588)107107959X gnd |
| topic_facet | Leukocyte Elastase Chemokine Entzündung Myeloblastin Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028197229&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dautheresethuydung proteolyticmodificationofelastaseandchemokineactivitiesbyneutrophilelastaseandproteinase3 |