Characterization of drought tolerance in potato cultivars for identification of molecular markers:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 146 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Characterization of drought tolerance in potato cultivars for identification of molecular markers |c von Heike Sprenger |
| 264 | 1 | |c 2014 | |
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Datensatz im Suchindex
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| adam_text | CONTENTS
ZUSAMMENFASSUNG I
SUMMARY II
CONTENTS III
LIST OF FIGURES VI
LIST OF TABLES IX
ABBREVIATIONS XI
1 INTRODUCTION 1
1.1 ORIGIN AND IMPORTANCE OF POTATO 1
1.2 DROUGHT STRESS 2
1.2.1 IMPACT OF DROUGHT ON POTATO 2
1.2.2 DROUGHT STRESS RESPONSES IN PLANTS 4
1.2.2.1 PHOTOSYNTHESIS AND RESPIRATION 4
1.2.2.2 TRANSCRIPTIONAL REGULATION OF GENE EXPRESSION 5
1.2.2.3 REACTIVE OXYGEN SPECIES (ROS) 6
1.2.2.4 ACCUMULATION OF COMPATIBLE SOLUTES 7
1.2.2.5 PROTECTIVE PROTEINS 9
1.3 POTATO BREEDING FOR DROUGHT TOLERANCE 9
1.3.1 UTILIZATION OF WILD POTATO SPECIES 10
1.3.2 GENETIC ENGINEERING OF DROUGHT TOLERANCE IN POTATO 10
1.3.3 MARKER ASSISTED SELECTION 11
1.4 MOLECULAR BIOMARKER DISCOVERY USING OMICS DATA 13
1.4.1 TRANSCRIPT MARKERS 13
1.4.2 METABOLITE MARKERS 14
1.5 AIM OF THE THESIS 15
2 MATERIAL AND METHODS 16
2.1 MATERIAL 16
2.1.1 INSTRUMENTS 16
2.1.2 CONSUMABLES 16
III
HTTP://D-NB.INFO/1074115333
2.1.3 CHEMICALS 17
2.1.4 SOFTWARE 18
2.2 METHODS 19
2.2.1 PLANT MATERIAL 19
2.2.2 PLANT CULTIVATION AND GROWTH CONDITIONS 19
2.2.2.1 GREENHOUSE EXPERIMENTS 19
2.2.2.2 FIELD EXPERIMENTS 22
2.2.2.3 CULTIVATION UNDER AGRICULTURAL CONDITIONS 24
2.2.3 PHENOTYPIC AND PHYSIOLOGICAL CHARACTERIZATION OF PLANTS 24
2.2.3.1 GREENHOUSE EXPERIMENTS 24
2.2.3.2 FIELD EXPERIMENTS 25
2.2.4 YIELD DATA ANALYSIS 25
2.2.5 GENOTYPIC CHARACTERIZATION 26
2.2.6 SAMPLE PROCESSING 26
2.2.7 METABOLITE PROFILING BY GC-MS 26
2.2.8 TRANSCRIPT PROFILING 27
2.2.8.1 QUANTITATIVE REVERSE TRANSCRIPTION PCR (QRT-PCR) 27
2.2.8.2 QRT-PCR PRIMER DESIGN 28
2.2.8.3 RNA SEQUENCING 29
2.2.9 DATA WAREHOUSE 30
2.2.10 VISUALIZATION AND STATISTICAL ANALYSIS 31
2.2.11 MODELING OF DROUGHT TOLERANCE 31
3 RESULTS 33
3.1 IMPACT OF DROUGHT AND RE-WATERING AT DIFFERENT DEVELOPMENTAL STAGES
33
3.1.1 PHENOTYPIC AND PHYSIOLOGICAL CHARACTERIZATION 33
3.1.1.1 CONTROLLED GREENHOUSE EXPERIMENTS 33
3.1.1.2 CONTROLLED FIELD EXPERIMENTS 36
3.1.2 METABOLITE PROFILING 39
3.1.2.1 CONTROLLED GREENHOUSE EXPERIMENTS 40
3.1.2.2 CONTROLLED FIELD EXPERIMENT 45
3.2 DETAILED ANALYSIS OF DROUGHT TOLERANCE IN FOUR CULTIVARS 48
3.2.1 METABOLITE PROFILING 49
IV
3.2.2 TRANSCRIPT PROFILING 57
3.2.2.1 SAMPLE EVALUATION 57
3.2.2.2 DIFFERENTIAL GENE EXPRESSION ANALYSIS 59
3.2.2.3 GENE EXPRESSION OF SENSITIVE AND TOLERANT CULTIVARS 64
3.2.2.4 DROUGHT STRESS RESPONSE OF SENSITIVE AND TOLERANT CULTIVARS . 67
3.3 IDENTIFICATION OF MOLECULAR MARKERS FOR DROUGHT TOLERANCE 71
3.3.1 TOLERANCE CLASSIFICATION OF CULTIVARS 71
3.3.2 METABOLITE MARKERS FOR DROUGHT TOLERANCE 73
3.3.2.1 VARIABLE SELECTION FROM RANDOM FOREST 77
3.3.2.2 PREDICTION OF DROUGHT TOLERANCE FOR AGRONOMIC TRIALS 78
3.3.3 TRANSCRIPT MARKERS FOR DROUGHT TOLERANCE 79
3.3.3.1 SELECTION OF HOUSEKEEPING GENES FOR QRT-PCR 79
3.3.3.2 SELECTION OF MARKER CANDIDATE GENES FOR DROUGHT TOLERANCE . 80
3.3.3.3 PREDICTION OF DROUGHT TOLERANCE AND VALIDATION 82
3.3.4 COMBINED MARKERS FOR DROUGHT TOLERANCE 85
4 DISCUSSION 87
4.1 IMPACT OF DROUGHT AND RE-WATERING AT DIFFERENT DEVELOPMENTAL STAGES
87
4.1.1 MORPHOLOGIC AND PHENOTYPIC CHARACTERIZATION 87
4.1.2 METABOLITE PROFILING 89
4.2 DETAILED ANALYSIS OF DROUGHT TOLERANCE IN FOUR CULTIVARS 95
4.2.1 METABOLITE PROFILING 96
4.2.2 TRANSCRIPT PROFILING 100
4.3 IDENTIFICATION OF MOLECULAR MARKERS FOR DROUGHT TOLERANCE 104
4.4 OUTIOOK 110
5 BIBLIOGRAPHY 112
APPENDIX 132
ACKNOWLEDGEMENT 143
LIST OF PUBLICATIONS & PRESENTATIONS 144
EIDESSTATTLICHE ERKLARUNG 146
V
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| language | English |
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| physical | XI, 146 S. Ill., graph. Darst. |
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| spelling | Sprenger, Heike Verfasser (DE-588)1072577380 aut Characterization of drought tolerance in potato cultivars for identification of molecular markers von Heike Sprenger 2014 XI, 146 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2014 Stressresistenz (DE-588)4326741-5 gnd rswk-swf Kartoffelsorte (DE-588)4114152-0 gnd rswk-swf Trockenheit (DE-588)4186270-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Trockenheit (DE-588)4186270-3 s Stressresistenz (DE-588)4326741-5 s Kartoffelsorte (DE-588)4114152-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028150950&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sprenger, Heike Characterization of drought tolerance in potato cultivars for identification of molecular markers Stressresistenz (DE-588)4326741-5 gnd Kartoffelsorte (DE-588)4114152-0 gnd Trockenheit (DE-588)4186270-3 gnd |
| subject_GND | (DE-588)4326741-5 (DE-588)4114152-0 (DE-588)4186270-3 (DE-588)4113937-9 |
| title | Characterization of drought tolerance in potato cultivars for identification of molecular markers |
| title_auth | Characterization of drought tolerance in potato cultivars for identification of molecular markers |
| title_exact_search | Characterization of drought tolerance in potato cultivars for identification of molecular markers |
| title_full | Characterization of drought tolerance in potato cultivars for identification of molecular markers von Heike Sprenger |
| title_fullStr | Characterization of drought tolerance in potato cultivars for identification of molecular markers von Heike Sprenger |
| title_full_unstemmed | Characterization of drought tolerance in potato cultivars for identification of molecular markers von Heike Sprenger |
| title_short | Characterization of drought tolerance in potato cultivars for identification of molecular markers |
| title_sort | characterization of drought tolerance in potato cultivars for identification of molecular markers |
| topic | Stressresistenz (DE-588)4326741-5 gnd Kartoffelsorte (DE-588)4114152-0 gnd Trockenheit (DE-588)4186270-3 gnd |
| topic_facet | Stressresistenz Kartoffelsorte Trockenheit Hochschulschrift |
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