Proteomic profiling: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1295 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 501 S. Ill., graph. Darst. |
| ISBN: | 9781493925490 |
Internformat
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| 245 | 1 | 0 | |a Proteomic profiling |b methods and protocols |c ed. by Anton Posch |
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: Proteomic profiling
Autor: Posch, Anton
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xj
1 Mechanical/Physical Methods of Cell Distribution
and Tissue Homogenization...................................... 1
Stanley Goldberg
2 Sample Preservation Through Heat Stabilization of Proteins:
Principles and Examples......................................... 21
Mats Boren
3 Isolating Peripheral Lymphocytes by Density Gradient Centrifugation
and Magnetic Cell Sorting....................................... 33
Frederic Brosseron, Katrin Marcus, and Caroline May
4 Investigating the Adipose Tissue Secretome: A Protocol
to Generate High-Quality Samples Appropriate for Comprehensive
Proteomic Profiling............................................. 43
Simon Goddeke, J org Kotzka, and Stefan Lehr
5 Methods for Proteomics-Based Analysis of the Human Muscle Secretome
Using an In Vitro Exercise Model.................................. 55
Mika Scheler, Martin Hrabe de Angelis, Hadi Al-Hasani,
Hans-Ulrich HUring, Cora Weigert, and Stefan Lehr
6 Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis
of Microbial Infection and Host-Pathogen Interactions................. 65
Yanbao Yu and Rembert Pieper
7 A Protocol for the Parallel Isolation of Intact Mitochondria
from Rat Liver, Kidney, Heart, and Brain............................ 75
Sabine Schulz, Josef IAchtmannegger, Sabine Schmitt, Christin Leitzinger,
Carola Eberhagen, Claudia Einer, Julian Kerth, Michaela Aichler,
and Hans Zischka
8 Isolation of Mitochondria from Cultured Cells and liver Tissue
Biopsies for Molecular and Biochemical Analyses....................... 87
Sabine Schmitt, Carola Eberhagen, Susanne Weber, Michaela Aichler,
and Hans Zischka
9 Dynamic Range Compression with ProteoMiner™:
Principles and Examples......................................... 99
Lei Li
10 Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed
Paraffin-Embedded (FFPE) Tissue................................. 109
Omid Azimzadeh, Michael J. Atkinson, and Soile Tapio
vii
viii Contents
11 Full-Length Protein Extraction Protocols and Gel-Based Downstream
Applications in Formalin-Fixed Tissue Proteomics...................... 117
Alessandro Tanca, Sergio Uzzau, and Maria Filippa Addis
12 Enrichment of Low-Abundant Protein Targets by Immunoprecipitation
Upstream of Mass Spectrometry................................... 135
Barbara Kaboord, Suzanne Smith, Bhavin Patel, and Scott Meier
13 Principles of Protein Labeling Techniques............................ 153
Christian Obermaier, Anja Griebel, and Reiner Westermeier
14 Isolation of Extracellular Vesicles for Proteomic Profiling................ 167
Dong-Sic Choi and Yong Song Gho
15 A Protocol for Exosome Isolation and Characterization: Evaluation
of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity
Capture Methods.............................................. 179
David W. Greening, RongXu, Hong Ji, Bow J. Tauro,
and Richard J. Simpson
16 Chloroplast Isolation and Affinity Chromatography for Enrichment
of Low-Abundant Proteins in Complex Proteomes..................... 211
Roman G. Bayer, Simon Stael, and Markus Teige
17 Depletion of RuBisCO Protein Using the Protamine Sulfate
Precipitation Method........................................... 225
Ravi Gupta and Sun Tae Kim
18 Step-by-Step Preparation of Proteins for Mass Spectrometric Analysis....... 235
Thomas Franz and Xinping Li
19 Identification of Protein N-Termini Using TMPP or Dimethyl Labeling
and Mass Spectrometry.......................................... 249
Jingjing Deng, Guoan Zhang, Fang-Ke Huang, and Thomas A. Neubert
20 Optimization of Cell Lysis and Protein Digestion Protocols
for Protein Analysis by LC-MS/MS................................ 259
Dominic Winter, Alireza Dehghani, and Hanno Steen
21 Comprehensive Protocol to Simultaneously Study Protein
Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation....... 275
Marcella Nunes Melo-Braga, Maria Ibdnez-Vea, Martin R0sselLarsen,
and Katarzyna Kulej
22 Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing...... 293
Giuseppina Maccarrone and Michaela D. Filiou
23 Principles and Examples of Gel-Based Approaches
for Phosphoprotein Analysis...................................... 305
Birgit Steinberger and Corina Mayrhofer
24 Neutral Phosphate-Affinity SDS-PAGE System for Profiling
of Protein Phosphorylation....................................... 323
Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
25 Enrichment and Identification of Bacterial Glycopeptides
by Mass Spectrometry........................................... 355
Nichollas E. Scott and Stuart J. Cordwell
Contents ix
26 In-Gel Peptide IEF Sample Preparation for LC/MS Analysis.............. 369
Tom Berkelman, Sricharan Bandhakavi, and Aran Paulus
27 Western Blotting Using In-Gel Protein Labeling as a Normalization
Control: Stain-Free Technology................................... 381
Jennifer E. Gilda and Aldrin V. Gomes
28 2-D Western Blotting for Evaluation of Antibodies Developed
for Detection of Host Cell Protein................................. 393
Tom Berkelman, Adriana Harbers, and Sricharan Bandhakavi
29 Free Flow Electrophoresis for Separation of Native Membrane
Protein Complexes............................................. 415
Lutz Andreas Eichacker, Gerhard Weber, Ute Sukop-Ktippel,
and Robert Wildgruber
30 Three-Dimensional Electrophoresis for Quantitative Profiling
of Complex Proteomes.......................................... 427
Sergio Mauro, Bertrand Colignon, Marc Dieu, Edouard Delaive,
and Martine Raes
31 A Bead-Based Multiplex Sandwich Immunoassay to Assess
the Abundance and Posttranslational Modification State of p-Catenin....... 441
Nicola Groll, Cornelia Sommersdorf, Thomas O. Joos, and Oliver Poetz
32 Identification of SUMO E3 Ligase-Specific Substrates Using the HuProt
Human Proteome Microarray..................................... 455
Eric Cox, Ijeoma XJzoma, Catherine Guzzo, Jun Seop Jeong,
Michael Matunis, Seth Blackshaw, and Heng Zhu
33 Amyloid-Binding Proteins: Affinity-Based Separation,
Proteomic Identification, and Optical Biosensor Validation............... 465
Alexei Medvedev, Olga Buneeva, Arthur Kopylov, Oksana Gnedenko,
Alexis Ivanov, Victor Zgoda, and Alexander A. Makarov
34 Proteomic Profiling by Nanomaterials-Based Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry for High-Resolution
Data and Novel Protein Information Directly from Biological Samples...... 479
Suresh Kumar Kailasa and Hui-Fen Wu
Index
497
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| spelling | Proteomic profiling methods and protocols ed. by Anton Posch New York, NY [u.a.] Humana Press 2015 XV, 501 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1295 Springer protocols Methode (DE-588)4038971-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteomanalyse (DE-588)4596545-6 s Methode (DE-588)4038971-6 s b DE-604 Posch, Anton edt Erscheint auch als Online-Ausgabe 978-1-4939-2550-6 Methods in molecular biology 1295 (DE-604)BV035362695 1295 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028020989&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Proteomic profiling methods and protocols Methods in molecular biology Methode (DE-588)4038971-6 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4596545-6 (DE-588)4143413-4 |
| title | Proteomic profiling methods and protocols |
| title_auth | Proteomic profiling methods and protocols |
| title_exact_search | Proteomic profiling methods and protocols |
| title_full | Proteomic profiling methods and protocols ed. by Anton Posch |
| title_fullStr | Proteomic profiling methods and protocols ed. by Anton Posch |
| title_full_unstemmed | Proteomic profiling methods and protocols ed. by Anton Posch |
| title_short | Proteomic profiling |
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