Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
München
Verl. Dr. Hut
2015
|
| Edition: | 1. Aufl. |
| Series: | Medizin
|
| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Physical Description: | XV, 152 S. Ill., graph. Darst. 210 mm x 148 mm, 354 g |
| ISBN: | 9783843920643 3843920648 |
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| 100 | 1 | |a Gräf, Patricia |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |c Patricia Gräf |
| 250 | |a 1. Aufl. | ||
| 263 | |a 201504 | ||
| 264 | 1 | |a München |b Verl. Dr. Hut |c 2015 | |
| 300 | |a XV, 152 S. |b Ill., graph. Darst. |c 210 mm x 148 mm, 354 g | ||
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| 490 | 0 | |a Medizin | |
| 502 | |a Zugl.: München, Technische Universität, Diss., 2014 | ||
| 653 | |a Serial transfer | ||
| 653 | |a immunocompetence | ||
| 653 | |a central memory T cells | ||
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Record in the Search Index
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| adam_text |
CONTENTS
INDEX OF FIGURES VII
ABBREVIATIONS XI
1INTRODUCTION 1
1.1
THE IMMUNE
SYSTEM 1
1.1.1 T CELL IMMUNITY 3
1.1.2 THE HETEROGENEITY OF CD8* CYTOTOXIC T CELLS, SUBSETS AND THE
DIVISION OF LABOR. 4
1.1.3 THE FIINCTIONAL AND DEVELOPMENTAL CAPACITIES OF MEMORY T CELL
SUBSETS. 8
1.2 T CELL DIFFERENTIATION,
LLNEAGE RELATION AND ANALOGY TO TISSUE 'STEMNESS' 10
1.2.1 MODELS OF CD8*
T CELL DIFFERENTIATION 10
1.2.2 INTRINSIC VARIABILITY AND ENVIRONMENTAL CUES_ - 13
1.2.3 ANALOGY TO HOMEOSTASIS IN OTHER CYCLING TISSUES 16
1.2.4 THE CONCEPT OF T CELL TISSUE STEM CELLS 18
1.3 ADOPTIVE
T CELL
THERAPY 22
1.3.1 T CELL THERAPY IN VIRAL INFECTIONS 22
1.3.2 T CELL THERAPY IN CANCER 24
1.3.3 FINDING THE RIGHT TARGET POPULATION FOR T CELL THERAPY 25
2 AIM OF THIS PHD THESIS 27
3 MATERIALS AND METHODS 29
3.1 MATERIALS 29
3.1.1 EQUIPMENT _ 29
3.1.2 CHEMICALS AND REAGENTS 30
3.1.3 BUFFERS AND MEDIA 32
3.1.4 ANTIBODIES 33
3.1.5 PEPTIDES 34
3.1.6 MHC MULUEMERS 35
3.1.7 MICE._ 35
3.1.8 SOFTWARE -35
III
HTTP://D-NB.INFO/1076497527
CONTENTS
3.2 METHODS 36
3.2.1 PREPARATION OF CELLS FROM DIFFERENT ORGANS 36
3.2.2 PRODUCTION OF MHC-1 MULTIMERS . 37
3.2.3 MULTIMERIZATION 37
3.2.4 ANTIBODY AND MULTIMER
STAINING.
. 37
3.2.5 INTRACELLULAR ANTIBODY STAINING 37
3.2.6 FACS ACQUISITUM AND ANALYSIS 38
3.2.7 FACS SORTING 38
3.2.7.1 PURIFLCATION OF NAIVE CD8* T CELLS 39
3.2.7.2 PURIFLCATION OF MEMORY CD8+ T CELLS 39
3.2.8 ADOPTIVE CELL TRANSFERS 40
3.2.9 INFECTIONS AND IMMUNIZATIONS 40
3.2.9.1 LISTERIA MONOCYTOGENES INFECTION 40
3.2.9.2 MODIFIED VACCINIA VIRUS
ANKARA IMMUNIZATION. 40
3.2.10 DETERMINATION OF BACTERIAL BUERDEN 41
3.2.11 STATISTICS 41
4 RESULTS 43
4.1 LONG-UVED
MEMORY AFTER
ACUTE INFECTION CONSISTS OF RESTING
CD8+ CD44
W
*
H
CENTRAL MEMORY AND
EFFECTOR MEMORY
T CELLS
4.2 EVALUATION OF THE DEVELOPMENTAL CAPACITY OF CD8+ CENTRAL
MEMORY AND
EFFECTOR MEMORY
T CELLS
.48
4.2.1 ESTABLISHMENT OF AN OPTIMAL STAINING PROCEDURA TO PURIFY
ANTIGEN-SPECIFIC CD8* MEMORY T CELLS
FOR ADOPTIVE RE^TRANSFER.
. 48
4.2.2 HIGH EXPANSION AND DIFFERENTIATION POTENTIAL OF CD8* CENTRAL
MEMORY T CELLS 52
4.3 EXTENDLNG
THE
SINGLE
CELL ADOPTIVE TRANSFER TO CD8* CENTRAL
MEMORY T CELLS
.56
4.3.1 ESTABLISHMENTOFA CONGENIC MATRIXOFCD8+ CENTRAL MEMORY T CELLS. 57
4.3.2 FURTHER EVALUATION OF AN OPTIMAL STAINING PROCEDURA TO PURIFY CD8+
CENTRAL MEMORY T CELLS FOR
ADOPTIVE RE-TRANSFER . . 61
4.3.3 SENSITIVITY OF CD8+ EFFECTOR
AND MEMORY T CELLS TO MEMORY MARKER STAINING 66
4.3.4 SIMILAR DEVELOPMENTAL CAPACITY OF CD8* NAIVE
AND CENTRAL MEMORY T CELLS 69
4.3.5 HIGH EXPANSION AND DIFFERENTIATION CAPACITY OF CD8* CENTRAL MEMORY
T CELLS FROM DIVERSE
ANATOMICAL ORIGIN 71
4.4 PRESERVATION OF KEY
DEVELOPMENTAL TRAITS BETWEEN SINGLE CDS* NAIVE
AND CENTRAL MEMORY
T CELLS
75
IV
CONTENTS
4.5 SERIAL ADOPTIVE TRANSFER AND INFECTION-DRIVEN RE-EXPANSION OF SINGLE
CELL-DERIVED IMMUNO-
COMPETENCE REVEALS STEMNESS OF INDIVIDUAL CDS
4
CENTRAL MEMORY T CELLS 79
4.5.1 ESTABLISHMENT OF TRANS-GENERATIONAL SINGLE CELL ADOPTIVE TRANSFERS
- 79
4.5.2 SERIAL ADOPTIVE TRANSFER OF SINGLE CELLS DEMONSTRATES FUNCTIONAL
SELF-RENEWAL CAPACITY OF
INDIVIDUAL CD8* CENTRAL MEMORY T CELLS 83
4.5.3 SINGLE CD8* CENTRAL MEMOIY T CELLS REMAIN MULTI-POTENT THROUGHOUT
SERIAL ADOPTIVE
TRANSFERS 87
4.5.4 PROGENY DERIVED FROM SINGLE CD8* CENTRAL MEMORY T CELLS CAN
RESTORE IMMUNO-COMPETENCE 90
5 DISCUSSION 95
5.1 FIRST FUNCTIONAL EVIDENCE FOR STEMNESS IN THE T CELL POOL 97
5.2 THE UNFORESEEN PRESERVATION OF KEY DEVELOPMENTAL TRALTS BETWEEN CD8*
NAIVE AND CENTRAL
MEMORY T CELLS 100
5.3 THE IMPACT ON T CELL DIFFERENTIATION AND MEMORY RELLABILITY 104
5.4 FUTURE PERSPECTIVES * 108
5.5 CLINICAL RELEVANCE 112
6 SUMMARY 115
7 ACKNOWLEOGEMENTS 117
8 REFERENCES 119
V |
| any_adam_object | 1 |
| author | Gräf, Patricia |
| author_facet | Gräf, Patricia |
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| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-08-03T02:23:37Z |
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| language | English |
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| physical | XV, 152 S. Ill., graph. Darst. 210 mm x 148 mm, 354 g |
| publishDate | 2015 |
| publishDateSearch | 2015 |
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| series2 | Medizin |
| spelling | Gräf, Patricia Verfasser aut Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf 1. Aufl. 201504 München Verl. Dr. Hut 2015 XV, 152 S. Ill., graph. Darst. 210 mm x 148 mm, 354 g txt rdacontent n rdamedia nc rdacarrier Medizin Zugl.: München, Technische Universität, Diss., 2014 Serial transfer immunocompetence central memory T cells (DE-588)4113937-9 Hochschulschrift gnd-content Verlag Dr. Hut (München) Sonstige (DE-588)10174118-2 oth X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=5206461&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028005588&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gräf, Patricia Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |
| subject_GND | (DE-588)4113937-9 |
| title | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |
| title_auth | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |
| title_exact_search | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |
| title_full | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf |
| title_fullStr | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf |
| title_full_unstemmed | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf |
| title_short | Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells |
| title_sort | serial transfer of single cell derived immunocompetence reveals stemness of cd8 central memory t cells |
| topic_facet | Hochschulschrift |
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