Methods in cilia & flagella:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Academic Press
2015
|
| Edition: | 1. ed. |
| Series: | Methods in cell biology
127 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXII, 567 S., [12] Bl. Ill., graph. Darst. |
| ISBN: | 9780128024515 |
Staff View
MARC
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| 300 | |a XXII, 567 S., [12] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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Record in the Search Index
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| adam_text |
Contents
Contributors
.xiii
Preface
.xxi
CHAPTER
1
Imaging Centrosomes and Cilia in the Mouse
Kidney
.1
Masato Hoshi, Jinzhi Wang, Sanjay Jain,
Мое
R.
Mahjoub
Introduction
.2
1.
Methods
.4
Conclusion
.13
References
.14
CHAPTER
2
Ependymal Cell Differentiation, from Monociliated
to Multiciliated Cells
.19
Nathalie Delgehyr, Alice
Meunier,
Marion Faucourt,
Montserrat
Bosch
Grau, Laetitia
Strehi,
Carsten Janke,
Nathalie
Spassky
Introduction.
20
1.
Methods
.20
Conclusion
.32
Acknowledgments
.32
References
.32
CHAPTER
3
Observing Planar Cell Polarity in Multiciliated
Mouse Airway Epithelial Cells
.37
Eszter
К.
Vladar,
Yin
Loon
Lee,
Tim
Stearns,
Jeffrey
Đ.
Axelrod
Introduction.
38
1.
Acquisition or Generation of Multiciliated Airway Epithelial
Cells
.41
2.
Assessment of Ultrastructural Planar Polarity of Motile Cilia.
42
3.
Localization of
PCP
Signaling Pathway Proteins in
Multiciliated Airway Epithelial Cells
.48
Conclusions
.52
Acknowledgments
.52
References
.52
vi
Contents
CHAPTER
4
Imaging and Analyzing Primary Cilia in Cardiac
Cells
.55
Nicolas Diguet,
Jean-François
Le Garree, Tommaso
Lucchesi,
Sigolène M. Meilhac
Introduction
.56
1.
Imaging Cilia In situ, in Fixed Hearts
.57
2.
Imaging and Manipulating Cilia in Primary Cultures
of Cardiac Cells
.61
3.
Quantitative Analysis of Cilium Orientation
.67
Conclusion
.72
Acknowledgments
.72
Supplementary Data
.72
References
.72
CHAPTER
5
Cilia in Photoreceptors
.75
Linjing Li,
Manistia Anand,
Kollu Nageswara Rao,
Hemant Khanna
Introduction
.76
1.
Methods
.77
Conclusions
.91
Acknowledgments
.91
References
.92
CHAPTER
6
Analysis of Primary Cilia in the Developing
Mouse Brain
.93
Judith T.M.L Paridaen,
Wieland
B. Huttner,
Michaela Wilsch-Bräuninger
Introduction
.94
1.
Neurogenesis in the Developing Neocortex
.94
2.
Primary Cilia in the Developing Neocortex
.96
3.
Material
.97
4.
Methods
.103
Conclusion
.126
Acknowledgments
.126
References
.126
CHAPTER
7
In Vivo Investigation of Cilia Structure and
Function Using Xenopus
.131
Eric R. Brooks, John B. Wallingford
Introduction
.132
1.
A Brief Introduction to Xenopus Husbandry and
Manipulation
.133
Contents
vii
2.
Imaging of Cilia in Fixed Xenopus Embryos and Tissues
. 134
3.
Methods for Live Cell Analysis of Cilia in Xenopus
. 139
4.
Mounting Xenopus Embryos
. 142
5.
Image Acquisition
. 145
6.
Quantitative Analysis of Ciliary Properties
.151
Conclusion
.154
Acknowledgments
. 155
References
. 155
CHAPTER
8
A Quantitative Approach to Study Endothelial
Cilia Bending Stiffness During Blood Flow
Mechanodetection In Vivo
.
161
Francesco Boselli, Jacky G.
Goetz, Gilles Charvin,
Julien
Vermot
Introduction
. 162
1.
The Challenge of Measuring Ciliary Mechanics
. 162
2.
Live Imaging
. 164
3.
Image Processing
. 168
4.
Bending Rigidity Estimation through Modeling
. 168
5.
Material
. 172
Conclusion
. 172
Acknowledgments
. 173
References
. 173
CHAPTER
9
Quantitative Description of Fluid Flows Produced
by Left-Right Cilia in Zebrafish
.175
Craig Fox, M. Lisa Manning, Jeffrey D. Amack
Introduction
. 176
1.
Methods
.177
2.
Application
.183
Conclusion
. 185
Acknowledgments
. 185
References
. 185
CHAPTER
10
Efficient Live Fluorescence Imaging of
Intraflagellar Transport in Mammalian
Primary Cilia
.189
Hiroaki Ishikawa, Wallace F. Marshall
Introduction
. 190
1.
Preparation of Cells for IFT Imaging
. 191
2.
Live Imaging of IFT
. 193
viii Contents
3. Image Processing
and Data Analysis
.195
4.
Discussion.
198
Acknowledgments
.199
Supplementary Data
.199
References
.199
CHAPTER
11
Analysis of Soluble Protein Entry Into Primary
Cilia Using Semipermeabilized Cells
.203
David K. Breslow, Maxence V. Nachury
Introduction
.204
1.
Methods
.205
2.
Conclusions and Future Outlook
.219
Acknowledgments
.219
References
.220
CHAPTER
12
Methods for Imaging Individual Cilia in Living
Echinoid Embryos
.223
Robert L. Morris, Hans W. Pope, Adam
N.
Sholi,
Leah M. Williams, Chelsea R. Ettinger,
Gwendolyn M. Beacham,
Tatsushi Shintaku,
Zachary D. Abbott, Elyse M. Doherty
Introduction
.224
1,
Methods
.225
Conclusion
.240
Acknowledgments
.240
Supplementary Data
.240
References
.240
CHAPTER
13
The
planarian
Schmidtea
Mediterranea
as a
Model for Studying Motile Cilia and
Multiciliated Cells
.243
Cyril Basquin, Anne-Marie Orfila, Juliette Azimzadeh
Introduction
.244
1.
Maintaining
Planarians
in the Lab
.247
2.
RNA
Interference
.251
3.
Whole-Mount Immunofluorescence Staining
.255
4.
Electron Microscopy
.258
5.
Live Imaging
.260
Acknowledgments
.261
References
.261
Contents ix
CHAPTER
14 Quantitative
Analysis of
Flagellar
Proteins in
Drosophila
Sperm Tails
.263
Teresa
Mendes
Maia,
Perrine Paul-Gilloteaux,
Renata
Basto
Introduction
.264
1.
Methods
.266
Concluding Remarks
.276
Acknowledgments
.276
References
.276
CHAPTER
15
Imaging Cilia in
Drosophila
melanogaster
.279
Jennifer
Vieillard, Jean-Luc
Duteyrat, Elisabeth Cortier,
Bénédicte Durand
Introduction
.280
1.
Immunodetection of Cilia
.283
2.
Observation of Cilia
Ultrastructure
by Transmission Electron
Microscopy
.293
Acknowledgments
.298
References
.298
CHAPTER
16
Studying
G
Protein-coupled Receptors:
Immunoblotting, Immunoprecipitation,
Phosphorylation, Surface Labeling, and
Cross-linking Protocols
.
зоз
Kasturi Pal, Hemant Badgandi, Saikat Mukhopadhyay
Introduction
.305
1.
Reagents
.307
2.
Protocol for Immunoblotting GPCRs
.311
3.
Protocol for Immunoprecipitating GPCRs
.313
4.
GPCR Labeling and Cross-Linking Studies
.317
5.
Comments on Biochemical Assays Involving GPCRs and
Multipass
Transmembrane
Proteins
.319
Conclusions
.320
Acknowledgments
.320
References
.320
CHAPTER
17
image Analysis of Caenorhabditis
elegans
Ciliary Transition Zone Structure,
Ultrastructure,
Molecular Composition, and Function
.
323
Anna A.W.M. Sanders, Julie Kennedy, Oliver E. Blacque
Introduction
.324
Contents
1. Cilium Compartmentalization
and the Transition
Zone.324
2. Caenorhabditis
elegans
Sensory
Neuronal
Cilia
Model.326
3.
Methods.
328
Concluding
Remarks
.344
References
.344
CHAPTER
18
Genetic and Genomic Approaches to Identify
Genes Involved in
Flagellar
Assembly in
Chlamydomonas
Reinhardt!!.349
Huawen Lin, Susan K. Dutcher
Introduction
.350
1.
Identifying the Causative Genes Using Forward Genetics
.351
2.
Identifying Gene Function Using Reverse Genetics
.370
3.
Identifying Genes Using the
RNA Transcriptome.
373
4.
Identifying Genes Using Genetic Interactions
.375
5.
Summary
.378
Acknowledgments
.378
References
.378
CHAPTER
19
Axonemal Motility in Chlamydomonas
.387
Ken-ichi Wakabayashi, Ritsu Kamiya
Introduction
.388
1.
Methods
.389
Acknowledgments
.400
References
.400
CHAPTER
20
Posttranscriptional Control Over Rapid
Development and Ciliogenesis in Marsilea
.403
Stephen M. Wolniak, Thomas
С
Boothby,
Corine
M.
van
der Weele
Introduction
.404
1.
The Development of Experimental Protocols in a
Nonmodel System
.409
2.
Centrin Translation is Necessary for Formation of the
Blepharoplast
.412
3.
Cell Fate in the Male Gametophyte of Marsilea: Only
Two Types of Cells are Produced
.415
4.
Spermidine Provides a Driving Force behind Rapid
Development in the Male Gametophyte
.417
5.
Spermidine Levels Change in the Gametophyte
.418
Contents xi
6.
Stored mRNAs are Transcribed during the Natural Process
of Spore Desiccation
.420
7.
Mago
Nashi and Speckle mRNA Dynamics
.423
8.
Emerging Themes from the
Transcriptome
of the Rapidly
Developing Male Gametophyte of Mar
silea
.423
9.
Many of the Stored Transcripts in the Spore Nucleus are
Partially Processed Pre-mRNAs
.425
10.
NudC, an Archetype
IRT
in the Gametophyte
.426
11.
IRT
Splicing as a Means to Regulate the Timing and
Location of Translation
.427
12.
Polyadeny
lation
as a Late Regulator of Translation
.430
Conclusions, Predictions, and a Working Model for the
Control of Rapid Development
.430
Acknowledgments
.438
References
.438
CHAPTER
21
Total Internal Reflection Fluorescence Microscopy
of Intraflagellar Transport in Tetrahymena
thermophila
.445
Yu-Yang Jiang, Karl Lechtreck,
Jacek
Gaertig
Introduction
.446
1.
TIRF Microscope Configuration and Data Analysis in
Application to IFT in Tetrahymena
.447
2.
Using a TIRF microscope to Image Tetrahymena cilia
.447
3.
Limitations
.453
Acknowledgments
.454
Supplementary Data
.454
References
.454
CHAPTER
22
Ciliary Heterogeneity within a Single Cell: The
Рагатесіит
Model
.457
Anne Aubusson-Fleury, Jean Cohen, Michel Lemullois
1.
The Paramecium Model
.458
2.
Cilia in Paramecium Organization
.460
3.
Staining the Cilia with Antitubulin Antibodies
.465
4.
Protocols
.470
5.
Conclusion
.478
6.
Chemical Reagents and Buffers
.479
Acknowledgments
.479
References
.479
xii Contents
CHAPTER
23
Imaging Intraflagellar Transport in
Trypanosomes
.487
Julien
Santi-Rocca,
Nicolas Chenouard,
Cécile
Fort,
Thibault
Lagache,
Jean-Christophe
Olivo-Marin,
Philippe
Bastin
Introduction
.488
1.
Producing Trypanosomes Expressing Fluorescent
IFT Fusion Proteins
.490
2.
Visualization of IFT and Acquisition of Time-Lapse Videos
. 498
3.
Quantification of IFT
.500
Conclusion
.504
Acknowledgments
.504
Supplementary Data
.504
References
.504
CHAPTER
24
Scanning and Three-Dimensional Electron
Microscopy Methods for the Study of
Trypanosoma brucei and Leishmania
mexicana
Flagella.
.509
Eva Gluenz, Richard John Wheeler, Louise Hughes,
Sue Vaughan
Introduction
.510
1.
Rationale
.515
2.
Methods
.516
3.
Materials
.535
4.
Discussion
.537
Acknowledgments
.538
References
.538
Volumes in Series
.543
Index
.555 |
| any_adam_object | 1 |
| author2 | Basto, Renata |
| author2_role | edt |
| author2_variant | r b rb |
| author_facet | Basto, Renata |
| building | Verbundindex |
| bvnumber | BV042549621 |
| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)907572157 (DE-599)BVBBV042549621 |
| discipline | Biologie |
| edition | 1. ed. |
| format | Book |
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| id | DE-604.BV042549621 |
| illustrated | Illustrated |
| indexdate | 2024-10-14T14:14:51Z |
| institution | BVB |
| isbn | 9780128024515 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027983531 |
| oclc_num | 907572157 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T DE-11 DE-703 |
| owner_facet | DE-355 DE-BY-UBR DE-29T DE-11 DE-703 |
| physical | XXII, 567 S., [12] Bl. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Elsevier, Academic Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Methods in cilia & flagella ed. by Renata Basto ... 1. ed. Amsterdam [u.a.] Elsevier, Academic Press 2015 XXII, 567 S., [12] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 127 Methode (DE-588)4038971-6 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf Zilie (DE-588)4190826-0 gnd rswk-swf Geißel Biologie (DE-588)4156348-7 gnd rswk-swf Zilie (DE-588)4190826-0 s Cytologie (DE-588)4070177-3 s Methode (DE-588)4038971-6 s DE-604 Geißel Biologie (DE-588)4156348-7 s b DE-604 Basto, Renata edt Methods in cell biology 127 (DE-604)BV002534878 127 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027983531&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Methods in cilia & flagella Methods in cell biology Methode (DE-588)4038971-6 gnd Cytologie (DE-588)4070177-3 gnd Zilie (DE-588)4190826-0 gnd Geißel Biologie (DE-588)4156348-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4070177-3 (DE-588)4190826-0 (DE-588)4156348-7 |
| title | Methods in cilia & flagella |
| title_auth | Methods in cilia & flagella |
| title_exact_search | Methods in cilia & flagella |
| title_full | Methods in cilia & flagella ed. by Renata Basto ... |
| title_fullStr | Methods in cilia & flagella ed. by Renata Basto ... |
| title_full_unstemmed | Methods in cilia & flagella ed. by Renata Basto ... |
| title_short | Methods in cilia & flagella |
| title_sort | methods in cilia flagella |
| topic | Methode (DE-588)4038971-6 gnd Cytologie (DE-588)4070177-3 gnd Zilie (DE-588)4190826-0 gnd Geißel Biologie (DE-588)4156348-7 gnd |
| topic_facet | Methode Cytologie Zilie Geißel Biologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027983531&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT bastorenata methodsinciliaflagella |