Rhodopsin: Methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1271 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 414 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Rhodopsin |b Methods and protocols |c ed. by Beata Jastrzebska |
| 264 | 1 | |a New York, NY [u.a.] |b Humana Press |c 2015 | |
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Datensatz im Suchindex
| _version_ | 1804153313389707264 |
|---|---|
| adam_text | Titel: Rhodopsin
Autor: Jastrzebska, Beata
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xi
Part I Historical Overview
1 The G Protein-Coupled Receptor Rhodopsin: A Historical Perspective...... 3
Lukas Hofinann and KrzysztofPalczewski
Part II Rhodopsin Expression, Regeneration, and Purification
FOR Structural Studies
2 Rhodopsin Purification from Dark-Adapted Bovine Retina............... 21
Elise Blankenship and David T. Lodowski
3 Mammalian Expression, Purification, and Crystallization
of Rhodopsin Variants........................................... 39
Daniel Mattle, Ankita Singhai, Georg Schmid, Roger Dawson,
and Jörg Standfuss
4 Imaging of Rhodopsin Crystals with Two-Photon Microscopy............ 55
Grazyna Palczewska and David Salom
Part III Structure-Function Characterization of Rhodopsin
5 Functional Stability of Rhodopsin in a Bicelle System:
Evaluating G Protein Activation by Rhodopsin in Bicelles................ 67
Ali I. Kaya, T.M. Iverson, and Heidi E. Hamm
6 The Rhodopsin-Arrestin-1 Interaction in Bicelles...................... 77
QJuyan Chen, Sergey A. Vishnivetskiy, Tiandi Zhuang, Min-Kyu Cho,
Tarjani M. Thaker, Charles PL Sanders, Vsevolod V. Gurevich,
and T.M. Iverson
7 Detection of Structural Waters and Their Role in Structural Dynamics
of Rhodopsin Activation......................................... 97
Liwen Wang and Mark R. Chance
8 Probing Conformational Changes in Rhodopsin
Using Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry...... 113
Tivadar Orban and Taroslav Tsybovsky
9 Analysis of Conformational Changes in Rhodopsin
by Histidine Hydrogen-Deuterium Exchange......................... 123
David T. Lodowski and Masaru Miyagi
10 Investigation of Rhodopsin Dynamics in Its Signaling State
by Solid-State Deuterium NMR Spectroscopy......................... 133
Audrey V. Struts, Udeep Chawla, Suchithranga M.D.C. Per era,
and Michael F. Brown
vii
viii
Contents
11 Sequential Structural Changes in Rhodopsin Occurring
upon Photoactivation........................................... 159
Naoki Kimata, Andreyah Pope, Dawood Rashid, Philip J. Reeves,
and Steven O. Smith
12 Dynamic Single-Molecule Force Spectroscopy of Rhodopsin
in Native Membranes........................................... 173
Paul S.-H. Park and Daniel J. Müller
Part IV Rhodopsin Supramolecular Organization
and Its Complexes with Partner Proteins
13 High-Resolution Atomic Force Microscopy Imaging
of Rhodopsin in Rod Outer Segment Disk Membranes.................. 189
Patrick D. Bosshart, Andreas Engel, and Dimitrios Fotiadis
14 Detection of Rhodopsin Dimerization In Situ by PIE-FCCS,
a Time-Resolved Fluorescence Spectroscopy.......................... 205
Adam W. Smith
15 Oligomeric State of Rhodopsin Within Rhodopsin-Transducin Complex
Probed with Succinylated Concanavalin A............................ 221
Beata Jastrzebska
16 Quantification of Arrestin-Rhodopsin Binding Stoichiometry............. 235
Ciara C.M. Lally and Martha E. Sommer
17 Rhodopsin Transient Complexes Investigated by Systems
Biology Approaches............................................ 251
Daniele Dell Orco
Part V Rhodopsin and Photoreceptors
18 Three-Dimensional Architecture of Murine Rod Cilium Revealed
by Cryo-EM.................................................. 267
Theodore G. Wensel and Jared C. Gilliam
19 Monitoring of Rhodopsin Trafficking and Mistrafficking
in Live Photoreceptors.......................................... 293
Kerrie H. Lodowski and Toshikazu Imanishi
20 Measurements of Rhodopsin Diffusion Within Signaling Membrane
Microcompartments in Live Photoreceptors.......................... 309
Mehdi Najafi and Peter D. Calvert
Part VI Treatment Strategies of Retinal Degenerative Disease
21 Kinetics of Rhodopsin s Chromophore Monitored
in a Single Photoreceptor........................................ 327
Leopold Adler TV, Nicholas P. Boyer, Chunhe Chen, and Tiannis Koutalos
22 Supplementation with Vitamin A Derivatives to Rescue Vision
in Animal Models of Degenerative Retinal Diseases..................... 345
Lindsay Perusek, Akiko Maeda, and Tadao Maeda
Contents ix
23 Sustained Delivery of Retinoids to Prevent Photoreceptor Death........... 363
Peter H. Tang and Rosalie K. Crouch
24 High-Throughput Screening Assays to Identify Small Molecules
Preventing Photoreceptor Degeneration Caused by the Rhodopsin
P23H Mutation............................................... 369
Tuanyuan Chen and Hong Tang
25 Gene Therapy to Rescue Retinal Degeneration Caused by Mutations
in Rhodopsin................................................. 391
Brian P. Rossmiller, Renee C. Ryals, and Alfred S. Lewin
Index............................................................... 411
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| illustrated | Illustrated |
| indexdate | 2024-07-10T01:24:37Z |
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| language | English |
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| physical | XIV, 414 S. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Rhodopsin Methods and protocols ed. by Beata Jastrzebska New York, NY [u.a.] Humana Press 2015 XIV, 414 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1271 Springer protocols Rhodopsine Biologie cellulaire Cytologie ram Jastrzebska, Beata Sonstige (DE-588)1071339532 oth Methods in molecular biology 1271 (DE-604)BV035362695 1271 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027980379&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rhodopsin Methods and protocols Methods in molecular biology Rhodopsine Biologie cellulaire Cytologie ram |
| title | Rhodopsin Methods and protocols |
| title_auth | Rhodopsin Methods and protocols |
| title_exact_search | Rhodopsin Methods and protocols |
| title_full | Rhodopsin Methods and protocols ed. by Beata Jastrzebska |
| title_fullStr | Rhodopsin Methods and protocols ed. by Beata Jastrzebska |
| title_full_unstemmed | Rhodopsin Methods and protocols ed. by Beata Jastrzebska |
| title_short | Rhodopsin |
| title_sort | rhodopsin methods and protocols |
| title_sub | Methods and protocols |
| topic | Rhodopsine Biologie cellulaire Cytologie ram |
| topic_facet | Rhodopsine Biologie cellulaire Cytologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027980379&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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