cAMP signaling: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J. [u.a.]
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1294 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | X, 222 S. Ill., graph. Darst. |
Internformat
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| 490 | 0 | |a Springer protocols | |
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Datensatz im Suchindex
| _version_ | 1804153312616906753 |
|---|---|
| adam_text | Titel: cAMP signaling
Autor: Zaccolo, Manuela
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Simultaneous Assessment of cAMP Signaling Events in Different Cellular
Compartments Using FRET-Based Reporters......................... 1
Alex Burdyga and Konstantinos Lefkimmiatis
2 Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors
by Fluorescence Lifetime Imaging.................................. 13
Marcel Raspe, Jeffrey Klarenbeek, and Kees Jalink
3 A Novel Approach Combining Real-Time Imaging and the Patch-Clamp
Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular
Microenvironment............................................. 25
Andreas Koschinski and Manuela Zaccolo
4 Structure-Based, In Silico Approaches for the Development
of Novel cAMP FRET Reporters................................... 41
Matias Machado and Sergio Pantano
5 Automated Image Analysis of FRET Signals for Subcellular
cAMP Quantification........................................... 59
Silas J. Leavesley, Arte Nakhmani, Ti Goo, and Thomas C. Rich
6 Channel-Based Reporters for cAMP Detection........................ 71
Thomas C. Rich, Wenkuan Xin, Silas J. Leavesley, and Mark S. Taylor
7 Imaging Sub-plasma Membrane cAMP Dynamics with Fluorescent
Translocation Reporters......................................... 85
Anders Tengholm and Olof Idevall-Hagren
8 Adenoviral Transduction of FRET-Based Biosensors for cAMP
in Primary Adult Mouse Cardiomyocytes............................ 103
Oliver Lomas, Marcella Brescia, Ricardo Carnicer, Stefania Monterisi,
Nicoletta C. Surdo, and Manuela Zaccolo
9 Generation of Transgenic Mice Expressing FRET Biosensors.............. 117
Daniela Hilbscher and Viacheslav O. Nikolaev
10 Photoactivatable Adenylyl Cyclases (PACs) as a Tool to Study cAMP
Signaling In Vivo: An Overview................................... 131
Marina Efetova and Martin Schwdrzel
11 Selective Disruption of the AKAP Signaling Complexes.................. 137
Eileen J. Kennedy and John D. Scott
12 Screening for Small Molecule Disruptors of AKAP-PKA Interactions....... 151
Carolin Schdchterle, Frank Christian, Jodo Miguel Parente Fernandes,
and Enno Klussmann
vii
viii
Contents
13 Structure-Based Bacteriophage Screening for AKAP-Selective PKA
Regulatory Subunit Variants...................................... 167
Ryan Walker-Gray and Matthew G. Gold
14 A Yeast-Based High-Throughput Screen for Modulators
of Phosphodiesterase Activity..................................... 181
Ana Santos de Medeiros and Charles S. Hoffman
15 Separation of PKA and PKG Signaling Nodes by Chemical Proteomics...... 191
Eleonora Corradini, Albert J,R. Heck, and Arjen Scholten
16 Development of Computational Models of cAMP Signaling.............. 203
Susana R. Neves-Zaph and Roy S. Song
Index............................................................... 219
|
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| discipline | Biologie |
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| id | DE-604.BV042545861 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:24:37Z |
| institution | BVB |
| language | English |
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| physical | X, 222 S. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | cAMP signaling methods and protocols ed. by Manuela Zaccolo Totowa, N.J. [u.a.] Humana Press 2015 X, 222 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1294 Springer protocols Includes bibliographical references and index Cyclic adenylic acid / Laboratory manuals Zaccolo, Manuela Sonstige (DE-588)1069954187 oth Methods in molecular biology 1294 (DE-604)BV035362695 1294 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027979845&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | cAMP signaling methods and protocols Methods in molecular biology Cyclic adenylic acid / Laboratory manuals |
| title | cAMP signaling methods and protocols |
| title_auth | cAMP signaling methods and protocols |
| title_exact_search | cAMP signaling methods and protocols |
| title_full | cAMP signaling methods and protocols ed. by Manuela Zaccolo |
| title_fullStr | cAMP signaling methods and protocols ed. by Manuela Zaccolo |
| title_full_unstemmed | cAMP signaling methods and protocols ed. by Manuela Zaccolo |
| title_short | cAMP signaling |
| title_sort | camp signaling methods and protocols |
| title_sub | methods and protocols |
| topic | Cyclic adenylic acid / Laboratory manuals |
| topic_facet | Cyclic adenylic acid / Laboratory manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027979845&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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