The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 129 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system |c vorgelegt von Isabell Koxholt |
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| 502 | |a Köln, Univ., Diss., 2014 | ||
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Record in the Search Index
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| adam_text | Titel: The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system
Autor: Koxholt, Isabell
Jahr: 2014
Index
1 Abstract/Zusammenfassung 7
2 Introduction 10
2.1 Basement membrane composition and function 10
2.2 The mammalian nidogen protein family 11
2.3 Nidogen deficient mouse models 13
2.4 Laminin-nidogen interaction 13
2.5 The morphology of the peripheral nervous system 16
2.6 Composition of basement membranes in the peripheral nervous system 17
2.7 Development of the peripheral nervous system 18
2.7.1 Interactions between axons and Schwann cells 19
2.7.2 Function of basement membrane components during peripheral
nervous system development 19
3 Materials and Methods 24
3.1 Materials 24
3.1.1 Chemicals 24
3.1.2 Antibodies 24
3.1.3 Enzymes and proteins 27
3.1.4 List of synthetic oligodesoxyribonucleotides (primer) and polymerase-
chain reaction (PCR)-programmes 27
3.1.5 Buffers and solutions 28
3.1.6 Media 30
3.2 Methods 31
3.2.1 Molecular biological analysis 31
3.2.2 Protein chemistry methods 31
3.2.3 In vitro analysis of dorsal root ganglia cells 34
3.2.4 Histological and indirect immunofluorescence methods 35
3.2.5 Morphometry analysis 37
3.2.6 Animal experiments 38
3.2.7 Graphics programs and statistical analysis 39
4 Results 40
4.1 Aims of this study 40
4.2 Mouse strains 40
4.2.1 Nidogen 1 deficient mice (NID1-/-) 40
4.2.2 Nidogen 2 deficient mice (NID2-/-) 41
4.2.3 Control groups 41
4.2.4 Genotyping of the mouse strains 41
4.3 Testing of motor behavior of the nidogen deficient mice 42
4.4 Analysis of basement membrane composition in the peripheral nervous system of
NID1-/- mice 44
4.4.1 Analysis of nidogen deposition in the basement membranes of
Schwann cells of NID1-/- mice 44
4.4.2 Analysis of laminin deposition in the basement membranes of
Schwann cells in NID1-/- mice 45
4.4.3 Analysis of other basement membrane and extracellular matrix
components of the peripheral nervous system in NID1-/- mice 46
4.4.4 Ultrastructural analysis of the basement membranes of Schwann
cells in NID1-/- mice 47
4.4.5 Extracellular matrix proteomics of sciatic nerve lysates of
NID1-/- mice (iTRAQ) 47
4.5 Morphological analysis of the sciatic nerves of NID1-/- mice 49
4.5.1 Methylene blue staining 49
4.5.2 Analysis of the plaque-like structures seen in NID1-/- sciatic nerves 50
4.5.3 Analysis of the Schwann cell phenotype and distribution in NID1-/- mice 50
4.5.4 Ultrastructural analysis of the sciatic nerves of NID1-/- mice 51
4.6 Analysis of the peripheral nervous system development in NID1-/- mice 52
4.6.1 Analysis of Schwann cell proliferation 52
4.6.2 Analysis of Schwann cell survival 54
4.6.3 Analysis of axonal sorting in W/Df-/-sciatic nerves 55
4.6.4 Analysis of the myelination process 57
4.6.5 Effect of nidogen 1 deficiency on signaling pathways required
for myelination 65
4.7 Sensory testing of nidogen deficient mice 69
4.7.1 In vitro analysis of dorsal root ganglia cells 70
4.8 Analysis of the lamy1N802S double mutant mice 73
4.8.1 Introduction of the lamy1N802S double mutant mouse strain 73
4.8.2 Genotyping 74
4.8.3 Basement membrane composition in different organs of
lamyl N802S double mutant mice 75
4.8.4 Morphological analysis of the sciatic nerves of lamyl N802S
double mutants 80
4.8.5 Analysis of peripheral nervous system development in
lamyl N802S double mutants 83
4.8.6 Comparison of alterations in signaling pathways of nidogen deficient
and lamyl N802S double mutant mice 87
5 Discussion 89
5.1 Neurological phenotypes only seen in the absence of nidogen 1 89
5.2 Role of nidogen 1 during PNS development 92
5.3 Is nidogen 1 required for basement membrane stability or has nidogen 1 a cellular
isoform-specific function in peripheral nerve development, maturation and
maintenance? 95
5.4 Sensory phenotypes of NID1-/- mice 99
5.5 Comparison of the neurological phenotypes of NID1-/- and lamylN802S double
mutants mice 101
6 Outlook 104
References 106
List of abbreviation 123
Acknowledgment 126
Erklärung 128
CV 129
|
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| discipline | Medizin |
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| spelling | Koxholt, Isabell 1984- Verfasser (DE-588)1066488320 aut The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system vorgelegt von Isabell Koxholt 2014 129 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2014 Zsfassung in dt. und engl. Sprache Basalmembran (DE-588)4144086-9 gnd rswk-swf Peripheres Nervensystem (DE-588)4173794-5 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Laminin (DE-588)4197625-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Basalmembran (DE-588)4144086-9 s Laminin (DE-588)4197625-3 s Peripheres Nervensystem (DE-588)4173794-5 s Maus (DE-588)4169148-9 s DE-188 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027923067&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Koxholt, Isabell 1984- The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system Basalmembran (DE-588)4144086-9 gnd Peripheres Nervensystem (DE-588)4173794-5 gnd Maus (DE-588)4169148-9 gnd Laminin (DE-588)4197625-3 gnd |
| subject_GND | (DE-588)4144086-9 (DE-588)4173794-5 (DE-588)4169148-9 (DE-588)4197625-3 (DE-588)4113937-9 |
| title | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system |
| title_auth | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system |
| title_exact_search | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system |
| title_full | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system vorgelegt von Isabell Koxholt |
| title_fullStr | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system vorgelegt von Isabell Koxholt |
| title_full_unstemmed | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system vorgelegt von Isabell Koxholt |
| title_short | The role of nidogen 1 and nidogen 1-laminin interaction in the peripheral nervous system |
| title_sort | the role of nidogen 1 and nidogen 1 laminin interaction in the peripheral nervous system |
| topic | Basalmembran (DE-588)4144086-9 gnd Peripheres Nervensystem (DE-588)4173794-5 gnd Maus (DE-588)4169148-9 gnd Laminin (DE-588)4197625-3 gnd |
| topic_facet | Basalmembran Peripheres Nervensystem Maus Laminin Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027923067&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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