Insoluble proteins: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1258 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 425 S. Ill., graph. Darst. |
| ISBN: | 9781493922048 9781493922055 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804153062436110336 |
|---|---|
| adam_text | Titel: Insoluble proteins
Autor: García-Fruitós, Elena
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xi
1 General Introduction: Recombinant Protein Production
and Purification of Insoluble Proteins............................... 1
Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corcherò,
Zhikun Xu, and Elena Garcia-Fruités
Part I Recombinant Protein Production in Escherichia coli
2 Overcoming the Solubility Problem in E. coli: Available
Approaches for Recombinant Protein Production...................... 27
Agustin Correa and Pablo Oppezzo
3 Optimization of Culture Parameters and Novel Strategies
to Improve Protein Solubility..................................... 45
Ranjana Arya, Jamal S.M. Sabir, Roop S. Bora,
and Kulvinder S. Saini
4 Cleavable Self-Aggregating Tags (cSAT) for Protein
Expression and Purification....................................... 65
Zhanglin Lin, Qing Zhao, Bihong Zhou, Lei Xing, and Wanghui Xu
5 Beyond the Cytoplasm of Escherichia coli: Localizing
Recombinant Proteins Where You Want Them........................ 79
Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi,
and Matthew P. DeLisa
6 Characterization of Amyloid-Like Properties in Bacterial
Intracellular Aggregates......................................... 99
Anna Villar-Pique, Susanna Navarro, and Salvador Ventura
Part II Strategies to Produce Insoluble Proteins
in Cell-Free Expression Systems
7 Co-translational Stabilization of Insoluble Proteins
in Cell-Free Expression Systems................................... 125
Lei Kai, Erika Orbdn, Erik Henrich, Davide Proverbio,
Volker Dötsch, and Frank Bernhard
Part III Recombinant Protein Production
in Lactic Acid Bacteria (LAB)
8 Functional Expression of Plant Membrane Proteins in Lactococcus lactis...... 147
Sylvain Boutigny, Enteline Sautron, Annie Frelet-Barrand,
Lucas Moyet, Daniel Salvi, Norbert Rolland,
and Daphné Seigneurin-Berny
vii
viii Contents
Part IV Recombinant Protein Production in Yeast
9 High Cell-Density Expression System: Yeast Cells in a Phalanx
Efficiently Produce a Certain Range of Difficult-to-Express
Secretory Recombinant Proteins................................... 169
Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito
Part V Recombinant Protein Production
in Insect Cells-Baculovirus
10 Insect Cells-Baculovirus System for the Production
of Difficult to Express Proteins.................................... 181
Judit Osz-Papai, Laura Radu, Wassim Abdulrahman,
Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Catherine Birck,
and Arnaud Poterszman
Part VI Recombinant Protein Production in Mammalian Cells
11 Transient Expression in HEK 293 Cells: An Alternative to E. coli
for the Production of Secreted and Intracellular Mammalian Proteins....... 209
Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq,
Louise Pairall, Mareike G. Posner, Abhishek Upadhyay,
Yamini Reddivari, Jonathan M.G. Chamberlain, Simon E. Kolstoe,
Stefan Bagby, John W.R Schwabe, and Raymond J. Owens
12 Recombinant Glycoprotein Production in Human Cell Lines............. 223
Kamilla Swiech, Marcela Cristina Correa de Freitas,
Dimas Tadeu Covas, and Virginia Picanço-Castro
Part VII Recombinant Protein Production in Other Systems
13 Soluble Recombinant Protein Production in Pseudoalteromonas
haloplanktis TAC125............................................ 243
Maria Giuliani, Ermenegilda Parrilli, Filomena Sannino,
Gennaro Apuzzo, Gennaro Marino, and Maria Luisa Tutina
Part VIII Insoluble Protein Purification
14 A Screening Methodology for Purifying Proteins with Aggregation
Problems.................................................... 261
Mario Lebendiker, Michal Maes, and Assaf Friedler
15 Solubilization and Refolding of Inclusion Body Proteins................. 283
Anupam Singh, Vaibhav Upadhyay, andAmulya K. Panda
16 Bacterial Inclusion Body Purification................................ 293
Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido,
Antonio Villaverde, and Elena Garcia-Fruitós
17 Characterization of Intracellular Aggresomes by Fluorescent Microscopy..... 307
Lianwu Fu and Elizabeth Sztul
Contents ix
Part IX Insoluble Protein Characterization
18 Dialysis: A Characterization Method of Aggregation Tendency........... . 321
Mireia Pesarrodona, Ugutz Unzueta, and Esther Vazquez
19 Applications of Mass Spectrometry to the Study of Protein Aggregation..... 331
Silvia Bronsoms and Sebastidn A. Trejo
20 Insoluble Protein Assemblies Characterized by Fourier
Transform Infrared Spectroscopy................................... 347
Antonino Natalello and Silvia M. Doglia
21 Insoluble Protein Characterization by Circular Dichroism (CD)
Spectroscopy and Nuclear Magnetic Resonance (NMR)................. 371
Shaveta Goyal, Haina Qin, Liangzhong Lim, and Jianxing Song
22 Methods for Characterization of Protein Aggregates.................... 387
Witold Tatkiewicz, Elisa Elizondo, Evelyn Moreno, Cesar Diez-Gil,
Nora Ventosa, Jaume Veciana, and Imma Ratera
23 Predicting the Solubility of Recombinant Proteins in Escherichia coli........ 403
Roger G. Harrison and Miguel J. Bagajewicz
Part X Insoluble Protein Applications
24 Insoluble Protein Applications: The Use of Bacterial Inclusion
Bodies as Biocatalysts........................................... 411
Eva Hrabdrovd, Lucia Achbergerovd, and JozefNahdlka
Index............................................................... 423
|
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| indexdate | 2024-07-10T01:20:38Z |
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| isbn | 9781493922048 9781493922055 |
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| physical | XVI, 425 S. Ill., graph. Darst. |
| publishDate | 2015 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Insoluble proteins methods and protocols ed. by Elena García-Fruitós New York, NY [u.a.] Humana Press 2015 XVI, 425 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1258 Springer protocols García-Fruitós, Elena edt Methods in molecular biology 1258 (DE-604)BV035362695 1258 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027840623&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Insoluble proteins methods and protocols Methods in molecular biology |
| title | Insoluble proteins methods and protocols |
| title_auth | Insoluble proteins methods and protocols |
| title_exact_search | Insoluble proteins methods and protocols |
| title_full | Insoluble proteins methods and protocols ed. by Elena García-Fruitós |
| title_fullStr | Insoluble proteins methods and protocols ed. by Elena García-Fruitós |
| title_full_unstemmed | Insoluble proteins methods and protocols ed. by Elena García-Fruitós |
| title_short | Insoluble proteins |
| title_sort | insoluble proteins methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027840623&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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