NF-kappa B: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1280 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XVI, 668 S. zahlr. Ill.; graph. Darst. |
| ISBN: | 9781493924219 |
Internformat
MARC
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| 020 | |a 9781493924219 |c hardback |9 978-1-4939-2421-9 | ||
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| 245 | 1 | 0 | |a NF-kappa B |b methods and protocols |c ed. by Michael J. May |
| 264 | 1 | |a New York |b Humana Press |c 2015 | |
| 300 | |a XVI, 668 S. |b zahlr. Ill.; graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 1280 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a NF-kappa B / Laboratory Manuals | |
| 650 | 4 | |a DNA-Binding Proteins | |
| 700 | 1 | |a May, Michael J. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 1280 |w (DE-604)BV035362695 |9 1280 | |
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Datensatz im Suchindex
| _version_ | 1804153057012875264 |
|---|---|
| adam_text | Titel: NF-kappa B
Autor: May, Michael J
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xiii
Part I Standard Approaches to Detect NF-kB Pathway Activation
1 Electrophoretic Mobility Shift Assay Analysis of NF-kB DNA Binding....... 3
Sitharam Ramaswami and Matthew S. Hayden
2 Detection of IkB Degradation Dynamics and IkB-a Ubiquitination......... 15
Petro Starokadomskyy and Ezra Burstein
3 Measurement of NF-kB Transcriptional Activity and Identification
of NF-kB m-Regulatory Elements Using Luciferase Assays............... 25
Patricia E. Collins, Christine O Carroll, and Ruaidhri J. Carmody
Part II Detection and Analysis of NF-kB Signaling
4 Assessing Sites of NF-kB DNA Binding Using Chromatin
Immunoprecipitation.....................................
Amy Colleran, Patricia E. Collins, and Ruaidhri J. Carmody
5 IKK Kinase Assay for Assessment of Canonical NF-kB
Activation in Neurons.....................................
Anca B. Mihalas and Mollie K. Mejfert
6 Analysis of Epidermal Growth Factor-Induced NF-kB Signaling.....
Changyinß Jianß and Xin Lin
7 Methods to Assess the Activation of the Alternative (Noncanonical)
NF-kB Pathway by Non-death TNF Receptors..................
Caroline Remouchamps and Emmanuel Dejardin
8 Systematic Detection of Noncanonical NF-kB Activation...........
Zhaoxia Qj* and Gutian Xiao
9 Noncanonical NF-kB Activation and SDF-1 Expression
in Human Endothelial Cells................................
Kelly A. McCorkell and Michael J. May
10 Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts
Carolyn M. Gray and Michael J. May
11 Dissecting NF-kB Signaling Induced by Genotoxic Agents via Genetic
Complementation of NEMO-Deficient 1.3E2 Cells..............
Shawn S. Jackson and Shißeki Miyamoto
Part III Methods to Study the Control of NF-kB Signaling
12 Visualizing TCR-Induced POLKADOTS Formation and NF-kB Activation
in the D10 T-Cell Clone and Mouse Primary Effector T Cells............. 219
Suman Paul and Brian C. Schaejer
ix
47
61
75
103
121
155
181
197
x Contents
13 Detection of Recombinant and Cellular MALT1 Paracaspase Activity....... 239
Daniel Nagel and Daniel Krappmann
14 TRAF Protein Function in Noncanonical NF-kB Signaling............... 247
Anna D. Reichardt, Jose Pindado, Shivam A. Zaver,
and Genhong Cheng
15 Roles of c-IAP Proteins in TNF Receptor Family Activation
of NF-kB Signaling............................................. 269
Eugene Varfolomeev, Tatiana Goncharov, and Domagoj Vucic
16 Elucidating Dynamic Protein-Protein Interactions and Ubiquitination
in NF-kB Signaling Pathways..................................... 283
Noula Shembade and Edward W. Harhaj
17 Immunoblot Analysis of Linear Polyubiquitination of NEMO............. 297
Toshiteru Sasaki, Hiroaki Fujita, Misa Nakai, and Kazuhiro Iwai
18 In Vitro Detection of NEMO-Ubiquitin Binding Using DELFIA
and Microscale Thermophoresis Assays.............................. 311
Michelle Vincendeau, Daniel Krappmann, and Kamyar Hadian
19 Use of Fluorescence Spectroscopy for Quantitative
Investigations of Ubiquitin Interactions with the Ubiquitin-Binding
Domains of NEMO............................................ 321
Virginie Dubosclard, Elisabeth Fontan, and Fabrice Agou
20 Generation of a Proteolytic Signal: E3/E2-Mediated Polyubiquitination
of IkBö...................................................... 339
Robert A. Chong, Kenneth Wu, Jordan Kovacev, and Zhen-Qiang Pan
21 Control of NF-kB Subunits by Ubiquitination........................ 355
Patricia E. Collins, Amy Colleran, and Ruaidhri J. Carmody
22 Methodology to Study NF-KB/RelA Ubiquitination In Vivo.............. 371
Haiying Li, Petro Starokadomskyy, and Ezra Burstein
23 Using Sequential Immunoprecipitation and Mass Spectrometry
to Identify Methylation of NF-KB.................................. 383
Too Lu and George R. Stark
24 Methods to Detect NF-KB Acetylation and Methylation................. 395
JinJing Chen and Lin-Feng Chen
Part IV Analyzing and Targeting NF-kB Activity in Disease
25 A Method for the Quantitative Analysis of Stimulation-Induced Nuclear
Translocation of the p65 Subunit of NF-KB from Patient-Derived
Dermal Fibroblasts............................................. 413
Alex W. Wessel and Eric P. Hanson
26 Methods for Assessing the In Vitro Transforming Activity
of NF-kB Transcription Factor c-Rel and Related Proteins................ 427
Thomas D. Gilmore and Céline Gélinas
27 Using RNA Interference in Lung Cancer Cells
to Target the IKK-NF-kB Pathway................................. 447
Daniela S. Bassères and Albert S. Baldwin
Contents xi
28 Immunohistochemical Analysis of NF-kB in Human Tumor Tissue......... 459
Clint T. Allen and Carter Van Waes
29 Assessment of Canonical NF-kB Activity in Canine Diffuse
Large B-Cell Lymphoma......................................... 469
Anita Gaurnier-Hausser and Nicola J. Mason
30 NEMO-Binding Domain Peptide Inhibition of Inflammatory
Signal-Induced NF-kB Activation In Vivo............................ 505
Kelly A, McCorkell and Michael J. May
31 Regulation of NF-kB Signaling in Osteoclasts and Myeloid Progenitors...... 527
Gaurav Swarnkar and TousefAbu-Amer
32 NF-kB Activation with Aging: Characterization and Therapeutic Inhibition. . . 543
Jinjj Zhao, Xuesen Li, Sara McGowan, Laura J. Niedernhofir,
and Paul D. Robbins
33 The Sneaking-Iigand Approach: Cell-Type Specific Inhibition
of the Classical NF-kB Pathway.................................... 559
Bettina Sehnert, Harald Burkhardt, Stefan Dübel, and Reinhard E. Voll
34 Sneaking-Ligand Fusion Proteins Attenuate Serum Transfer Arthritis by
Endothelium-Targeted NF-kB Inhibition............................ 579
Bettina Sehnert, Harald Burkhardt, Michael J. May, Jochen Zwerina,
and Reinhard E. Voll
35 Analysis of NF-kB Activation in Mouse Intestinal Epithelial Cells.......... 593
Helena Shaked, Monica Guma, and Michael Karin
Part V Bioinformatics and Modeling Approaches to Study NF-kB
36 Characterizing the DNA Binding Site Specificity
of NF-kB with Protein-Binding Microarrays (PBMs).................... 609
Trevor Singers, Thomas D, Gilmore, Brian Barron, and Ashley Penrose
37 Methods for Analyzing the Evolutionary Relationship of NF-kB Proteins
Using Free, Web-Driven Bioinformatics and Phylogenese Tools........... 631
John R. Einnerty and Thomas D. Gilmore
38 Studying NF-kB Signaling with Mathematical Models................... 647
Simon Mitchell, Rachel Tsui, and Alexander Hoffmann
Index............................................................... 663
|
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| indexdate | 2024-07-10T01:20:33Z |
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| isbn | 9781493924219 |
| language | English |
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| series | Methods in molecular biology |
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| spelling | NF-kappa B methods and protocols ed. by Michael J. May New York Humana Press 2015 XVI, 668 S. zahlr. Ill.; graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1280 Includes bibliographical references and index NF-kappa B / Laboratory Manuals DNA-Binding Proteins May, Michael J. Sonstige oth Methods in molecular biology 1280 (DE-604)BV035362695 1280 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027837309&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | NF-kappa B methods and protocols Methods in molecular biology NF-kappa B / Laboratory Manuals DNA-Binding Proteins |
| title | NF-kappa B methods and protocols |
| title_auth | NF-kappa B methods and protocols |
| title_exact_search | NF-kappa B methods and protocols |
| title_full | NF-kappa B methods and protocols ed. by Michael J. May |
| title_fullStr | NF-kappa B methods and protocols ed. by Michael J. May |
| title_full_unstemmed | NF-kappa B methods and protocols ed. by Michael J. May |
| title_short | NF-kappa B |
| title_sort | nf kappa b methods and protocols |
| title_sub | methods and protocols |
| topic | NF-kappa B / Laboratory Manuals DNA-Binding Proteins |
| topic_facet | NF-kappa B / Laboratory Manuals DNA-Binding Proteins |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027837309&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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