Molecular and ecological investigation of Haemosporida in bats:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
2014
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | II, 100 S. Ill., graph. Darst., Kt. |
Internformat
MARC
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| 245 | 1 | 0 | |a Molecular and ecological investigation of Haemosporida in bats |c von Juliane Schaer |
| 264 | 1 | |a Berlin |c 2014 | |
| 300 | |a II, 100 S. |b Ill., graph. Darst., Kt. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Humboldt-Univ., Diss., 2014 | ||
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Datensatz im Suchindex
| _version_ | 1804153036096929792 |
|---|---|
| adam_text | TABLE OF CONTENTS
SUMMARY
ZUSAMMENFASSUNG
EIDESSTATTLICHE ERKLARUNG
1 INTRODUCTION 1
1.1 CHIROPTERA 1
1.1.1 BAT DIVERSITY AND PHYLOGENY 1
1.1.2 BAT ECOLOGY 4
1.1.2.1 LIFE HISTORY 4
1.1.2.2 ECTOPARASITES 5
1.1.3 THE ROLE OF BATS AS PATHOGEN RESERVOIR HOSTS 5
1.2 MALARIA 6
1.2.1 HUMAN MALARIA 6
1.2.2 PARASITE LIFE CYCLE 8
1.2.3 RODENT MALARIA MODELS 10
1.3 HEMOSPORIDIAN PARASITES 11
1.3.1 OVERVIEW 11
1.3.2 TRADITIONAL
TAXONOMY 13
1.3.3 DEFINITION MALARIA PARASITE 14
1.3.4 MOLECULAR PHYLOGENIES 15
1.3.5 GENOME OF HEMOSPORIDIAN PARASITES 18
1.4 BAT MALARIA PARASITES 19
1.4.1 GENERA AND MORPHOSPECIES 19
1.4.2 PLASMODIUM 20
1.4.3 NYCTERIA 22
1.4.4 HEPATOCYSTIS 23
1.4.5 POLYCHROMOPHILUS 24
1.5 OBJECTIVES OF THIS THESIS 25
2 MATERIAL AND METHODS 27
2.1 MATERIAL 27
2.1.1 INVESTIGATED SPECIES 27
2.1.2 FIELD EQUIPMENT 28
2.1.3 LABORATORY EQUIPMENT 28
2.1.4 CONSUMABLES 29
HTTP://D-NB.INFO/1068085525
2.1.5 REAGENTS 29
2.1.6 COMMERCIAL KITS 30
2.1.7 SOLUTIONS, BUFFERS, ENZYMES 30
2.1.8 BACTERIA 30
2.1.9 OLIGONUCLEOTIDES 30
2.1.10 SOFTWARE AND DATABASES 30
2.2 M
ETHODS
31
2.2.1 FIELD METHODS 31
2.2.1.1 SAMPLING SITES 31
2.2.1.2 BAT CAPTURES 34
2.2.1.3 BAT IDENTIFICATIONS 36
2.2.1.4 BAT SAMPLING 36
2.2.1.5 PRESERVATION
OF SPECIMENS 37
2.2.2 MICROSCOPIC METHODS 38
2.2.2.1 GIEMSA-STAINED BLOOD
SMEARS 38
2.2.2.2 DETERMINATION OF
PARASITEMIA, SEX
RATIO,
WHITE
BLOOD
CELL
COUNT 39
2.2.2.3 HISTOLOGY 40
2.2.3 MOLECULAR METHODS 41
2.2.3.1 ISOLATION OF
HOST
AND
PARASITE GENOMIC
DNA 41
2.2.3.2 GENE
AMPLIFICATION BY
POLYMERASE CHAIN
REACTION 41
2.2.3.3 AGAROSE GEL
ELECTROPHORESIS 42
2.2.3.4 CLONING 42
2.2.3.5 DNA SEQUENCING 44
2.2.3.6 SEQUENCE
ALIGNMENTS 45
2.2.4 PHYLOGENETIC ANALYSES 45
3 RESULTS 48
3.1 PREVALENCE OF HEMOSPORIDIAN INFECTIONS 48
3.1.1 PREVALENCES ACROSS DIFFERENT SAMPLING SITES 51
3.2 H
IGH DIVERSITY OF
W
EST
A
FRICAN BAT MALARIA PARASITES
53
3.2.1 THREE-GENOME PHYLOGENY SEPARATES BAT HAEMOSPORIDA IN FOUR DISTINCT
GENERA 55
3.2.2
NYCTERIA
AS DISTINCT SISTER CLADE TO MAMMALIAN
PLASMODIUM/HEPATOCYSTIS
57
3.2.3
HEPATOCYSTIS-
HIGH PHYLOGENETIC DIVERSITY 57
3.2.4
POLYCHROMOPHILUS
PARASITES RESTRICTED TO MINIOPTERIDA/ESPERTILIONID HOSTS
59
3.2.5 TIGHT LINK OF BAT AND RODENT
PLASMODIUM
60
3.3
NYCTERIA PARASITE COEVOLUTION WITH
A
FROTROPICAL INSECTIVOROUS BATS
64
3.3.1 PREVALENCE ACROSS BAT HOST FAMILIES 64
3.3.2 TWO DISTINCT
NYCTERIA
LINEAGES IN RHINOLOPHID AND NYCTERID HOSTS 71
3.3.3
CO-PHYLOGENY OF NYCTERIA PARASITES AND THEIR HOSTS
72
3.4
HEPATOCYSTIS -
EVIDENCE FOR CONTINUOUS AND EFFICIENT TRANSMISSION CYCLES
75
3.4.1 HIGH PARASITEMIA LEVELS AND SEASONAL
PATTERN 75
3.4.2
PHYLOGENETIC DIVERSITY AND GENERALIST AMONG AFRICAN FRUIT BATS
80
3.4.3
LIVER AND SPLEEN HISTOLOGY
84
4 DISCUSSION 87
4.1 PREVALENCE OF MALARIA PARASITES IN BATS 87
4.1.1 HEMOSPORIDIAN PHYLOGENY IN CONTEXT WITH CHIROPTERAN PHYLOGENY 90
4.2
NYCTERIA
-
THE ONLY HEMOSPORIDIAN GENUS THAT INFECTS BAT GENERA OF BOTH
CHIROPTERAN SUBORDERS 91
4.2.1
A/VCTEHM-PREVALENCE IN BAT FAMILIES
91
4.2.2 ASSIGNMENT OF
NYCTERIA
PARASITES TO MORPHOSPECIES 91
4.2.3
PARASITEMIA OF NYCTERIA
92
4.2.4
SEPARATION OF NYCTERIA PARASITES IN TWO DISTINCT GROUPS
92
4.3
RODENT/BAT PLASMODIUM
93
4.3.1
RESEARCH OPPORTUNITIES OF A WILDLIFE PLASMODIUM/HOST SYSTEM
94
4.3.2
CHALLENGES TOWARDS A NEW MODEL ORGANISM FOR MALARIA RESEARCH
95
4.4
HEPATOCYSTIS * HIGH PREVALENCE AND DIVERSITY
95
4.5 PHYLOGENETIC PLACEMENT OF CHIROPTERAN HEMOSPORIDIAN PARASITES
97
4.6 METHODS AND CHALLENGES 98
4.6.1 SAMPLING LIMITATIONS 98
4.6.2 COMBINATION OF MICROSCOPY AND MOLECULAR METHODS 99
4.7
CONCLUSION AND OUTLOOK
100
REFERENCES
SUPPLEMENT
PUBLICATIONS
|
| any_adam_object | 1 |
| author | Schaer, Juliane 1982- |
| author_GND | (DE-588)106798707X |
| author_facet | Schaer, Juliane 1982- |
| author_role | aut |
| author_sort | Schaer, Juliane 1982- |
| author_variant | j s js |
| building | Verbundindex |
| bvnumber | BV042388577 |
| classification_rvk | XD 9500 |
| ctrlnum | (OCoLC)912468011 (DE-599)BVBBV042388577 |
| dewey-full | 571.99471194 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.99471194 |
| dewey-search | 571.99471194 |
| dewey-sort | 3571.99471194 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| spelling | Schaer, Juliane 1982- Verfasser (DE-588)106798707X aut Molecular and ecological investigation of Haemosporida in bats von Juliane Schaer Berlin 2014 II, 100 S. Ill., graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Berlin, Humboldt-Univ., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content Reproduziert als Schaer, Juliane Molecular and ecological investigation of Haemosporida in bats Ketsch bei Mannheim : Mikroform Dissertation, 2014 2 Mikrofiches (DE-604)BV042511443 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027824514&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schaer, Juliane 1982- Molecular and ecological investigation of Haemosporida in bats |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular and ecological investigation of Haemosporida in bats |
| title_auth | Molecular and ecological investigation of Haemosporida in bats |
| title_exact_search | Molecular and ecological investigation of Haemosporida in bats |
| title_full | Molecular and ecological investigation of Haemosporida in bats von Juliane Schaer |
| title_fullStr | Molecular and ecological investigation of Haemosporida in bats von Juliane Schaer |
| title_full_unstemmed | Molecular and ecological investigation of Haemosporida in bats von Juliane Schaer |
| title_short | Molecular and ecological investigation of Haemosporida in bats |
| title_sort | molecular and ecological investigation of haemosporida in bats |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027824514&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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