Molecular studies on hepatitis E viruses:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Giessen
Laufersweiler
2013
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Edition scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 114 S. Ill., graph. Darst. |
| ISBN: | 9783835960978 |
Internformat
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
I LIST OF ABBREVIATION
11NTRODUCTION 1
2LITERATURE REVIEW 1
2.1 TAXONOMY AND PHYTOGENY OF HEPEVIRIDAE 1
2.1.1 TAXONOMY AND PHYTOGENY. 1
2.2 EARLY HISTORY 3
2.3 MORPHOLOGY AND MOLECULAR BIOLOGY OF HEV 4
2.3.1 MORPHOLOGY AND GENOME ORGANIZATION 4
2.3.2 GENOME REPLICATION 5
2.3.3 VIRAL PARTICLE STRUCTURE 6
2.4 HEV INFECTION 7
2.4.1 MODE OF TRANSMISSION 7
2.4.2 BLOOD TRANSFUSION 8
2.4.3 CLINICAL DISEASE (HUMANS) 8
2.5 HEV IN ANIMALS 10
2.5.1 DOMESTIC PIGS 11
2.5.2 WILD BOAR AND DEER 13
2.5.3 OTHER ANIMALS SPECIES 14
2.6 PATHOGENESIS AND IMMUNE RESPONSE 15
2.7 DIAGNOSIS 16
2.7.1 SEROLOGICAL ASSAYS 17
2.7.2 RT-PCR AND QRT-PCR 18
2.8 EPIDEMIOLOGY 19
2.8.1 THE VIRTUAL EPIDEMIOLOGICAL TRANSITION 19
2.8.2 GEOGRAPHICAL DISTRIBUTION 19
2.8.3 ZOONOTIC ASPECTS OF HEV INFECTION 21
2.9 PREVENTION AND CONTROL 21
2.9.1 PREVENTION AND PROPHYLAXIS 21
2.9.2 VACCINES 22
3 MATERIALS AND METHODS 23
3.1 MATERIALS 23
3.1.1 CELLS 23
3.1.2 VIRUS AND ANTIBODIES 23
3.1.3 SAMPLES 24
3.1.4 ENZYMES AND ENZYME BUFFERS 32
3.1.5 PREFABRICATED MEDIA AND KITS 33
3.1.6 REAGENTS AND CHEMICALS 33
3.1.7 ALL-PURPOSE MEDIUM AND BUFFERS 34
3.1.8 MEDIA AND BUFFERS FOR CELL CULTURE 34
3.1.9 BUFFER FOR REVERSE TRANSCRIPTION (RT) AND POLYMERASE CHAIN
REACTION (PCR) 35
3.1.10 SOLUTIONS FOR DNA CLONING 36
3.1.11 SOLUTIONS FOR SDS PAGE AND PAGE-STAINING 36
3.1.12 BUFFERS FOR PROTEIN PURIFICATION (500 ML) 37
3.1.13 CONSUMABLES 37
3.1.14 INSTRUMENTS AND EQUIPMENT 38
V
HTTP://D-NB.INFO/1045956899
3.1.15 OLIGONUCLEOTIDES 39
3.1.16 REAL-TIME 41
3.2 METHODS 42
3.2.1 NUCLEIC ACID EXTRACTION 42
3.2.2 NUCLEIC ACID AMPLIFICATION 44
3.2.3 ANALYSIS OF PCR PRODUCTS BY AGAROSE GEL ELECTROPHORESIS 46
3.2.4 GEL EXTRACTION 47
3.2.5 CLONING 47
3.2.6 PLASMID PREPARATION 47
3.2.7 SEQUENCING 48
3.2.8 PHYLOGENETIC ANALYSIS 49
3.2.9 PROTEIN PURIFICATION 49
3.2.10 WORKING WITH CELL CULTURE 51
3.2.11 INFECTION OFA549 CELL LINE 51
4RESULTS 53
4.1 HEV IN DOMESTIC SWINE AND WILD BOAR 53
4.1.1 DETECTION OF HEV IN DOMESTIC SWINE 53
4.1.2 DETECTION OF HEV IN WILD BOAR 53
4.1.3 PHYLOGENETIC ANALYSIS 55
4.1.4 SEQUENCING OF THE COMPLETE CAPSID GENE AND PHYLOGENETIC ANALYSIS
OF HEV FROM
DOMESTIC SWINE AND WILD BOAR SAMPLES 58
4.1.5 SEARCH FOR RECOMBINANTS 61
4.1.6 COMPLETE SEQUENCE OF HEV ISOLATE FROM DOMESTIC SWINE 64
4.2 GENETIC VARIABILITY IN HEV ISOLATES 69
4.2.1 EVALUATION OF THE CURRENT SYSTEM 69
4.2.2 GROUPING OF HEV BASED ON COMPLETE GENOMIC SEQUENCES 72
4.2.3 HEV-LIKE VIRUSES 75
4.2.4 SUBTYPING OF GENOTYPES 3 AND 4 77
4.2.5 GEOGRAPHIC DISTRIBUTION 79
4.3 EXPRESSION OF HEV CAPSID PROTEIN 80
4.3.1 CONSTRUCTION OF RECOMBINANT CAPSID PROTEIN (ORF 2) IN PET VECTOR
81
4.3.2 EXPRESSION OF THE TWO CAPSID PROTEIN REGIONS 82
4.3.3 REACTIVITY OF FUSION PROTEINS 83
4.4 CULTIVATION OF HEV 84
4.4.1 REALTIME RT-PCR FOR HEV DETECTION 85
4.4.2 INFECTION EXPERIMENTS 85
5DISCUSSION 87
5.1. PREVALENCE OF HEPATITIS E VIRUS (HEV) IN SWINE AND WILD BOAR 87
5.1.1. PHYLOGENETIC ANALYSES
88
5.1.2. RECOMBINATION OF HEV 89
5.2. CLASSIFICATION OF HEV 90
5.3. DEVELOPMENT OF DIAGNOSTIC TOOLS 93
5.4. CULTIVATION OF HEV 93
5.4.1. INFECTION OF PRIMARY HEPATOCYTES 95
6SUMMARY 96
7ZUSAMMENFASSUNG 97
VI
8 REFERENCES 98
9 ACKNOWLEDGEMENTS 113
10CURRICULUM VITAE 114
VII
|
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| building | Verbundindex |
| bvnumber | BV042374770 |
| ctrlnum | (OCoLC)867766844 (DE-599)HEB335166598 |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV042374770 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:19:51Z |
| institution | BVB |
| isbn | 9783835960978 |
| language | English |
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| physical | IX, 114 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| publisher | Laufersweiler |
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| series2 | Edition scientifique |
| spelling | Filho, Edmilson Ferreira de Oliveira Verfasser aut Molecular studies on hepatitis E viruses by Edmilson Ferreira de Oliveira Filho 1. Aufl. Giessen Laufersweiler 2013 IX, 114 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Giessen, Univ., Diss., 2013 hepatitis E cabt animals cabt boar cabt zoonotic disease cabt cervine cabt rabbit cabt Zoonose (DE-588)4121949-1 gnd rswk-swf Hepatitis-E-Virus (DE-588)106909210X gnd rswk-swf Tiere (DE-588)4060087-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tiere (DE-588)4060087-7 s Hepatitis-E-Virus (DE-588)106909210X s Zoonose (DE-588)4121949-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027810964&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Filho, Edmilson Ferreira de Oliveira Molecular studies on hepatitis E viruses hepatitis E cabt animals cabt boar cabt zoonotic disease cabt cervine cabt rabbit cabt Zoonose (DE-588)4121949-1 gnd Hepatitis-E-Virus (DE-588)106909210X gnd Tiere (DE-588)4060087-7 gnd |
| subject_GND | (DE-588)4121949-1 (DE-588)106909210X (DE-588)4060087-7 (DE-588)4113937-9 |
| title | Molecular studies on hepatitis E viruses |
| title_auth | Molecular studies on hepatitis E viruses |
| title_exact_search | Molecular studies on hepatitis E viruses |
| title_full | Molecular studies on hepatitis E viruses by Edmilson Ferreira de Oliveira Filho |
| title_fullStr | Molecular studies on hepatitis E viruses by Edmilson Ferreira de Oliveira Filho |
| title_full_unstemmed | Molecular studies on hepatitis E viruses by Edmilson Ferreira de Oliveira Filho |
| title_short | Molecular studies on hepatitis E viruses |
| title_sort | molecular studies on hepatitis e viruses |
| topic | hepatitis E cabt animals cabt boar cabt zoonotic disease cabt cervine cabt rabbit cabt Zoonose (DE-588)4121949-1 gnd Hepatitis-E-Virus (DE-588)106909210X gnd Tiere (DE-588)4060087-7 gnd |
| topic_facet | hepatitis E animals boar zoonotic disease cervine rabbit Zoonose Hepatitis-E-Virus Tiere Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027810964&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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