Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache. - Online erschienen 2014 |
| Beschreibung: | XIII, 115 S. graph. Darst. |
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Datensatz im Suchindex
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|---|---|
| adam_text | STRUCTURAL DYNAMICS OF OLIGOPEPTIDES DETERMINED
BY FLUORESCENCE QUENCHING OF ORGANIC DYES
DISSERTATION ZUR ERLANGUNG DES
NATURWISSENSCHAFTLICHEN DOKTORGRADES
DER JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG
VORGELEGT VON
STEFAN BOILMANN
AUS
OELDE.
WURZBURG. 2013
HTTP://D-NB.INFO/1066698260
CONTENTS
1 INTRODUCTION 1
2
THEORY
7
2.1 ELECTRODYNAMICS 8
2.1.1 POLARIZABILITY 9
2.1.2 ABSORPTION AND
REFRACTION 9
2.2 DIFFUSIONAL PROPERTIES OF
PARTICLES 12
2.3 FLUORESCENT MOLECULES
13
2.3.1 QUANTUM MECHANICAL CONSIDERATIONS 13
2.3.2 FRANCK-CONDON PRINCIPLE
14
2.4 QUENCHING
MECHANISMS
OF FLUORESCENT ORGANIC
DYES 19
2.4.1 KINETIC ASPECTS
OF BIMOLECULAR
PROCESSES 19
2.4.2 ENERGY- AND ELECTRON TRANSFER 21
2.5 THE
SECOND ORDER
CORRELATION FUNCTION
OF FCS 28
2.6 UNSTRUCTURED POLYPEPTIDES
30
2.6.1 RATES OF POLYMER
DYNAMICS 31
2.6.2 MODIFICATION OF
BIOMOLECULES 32
2.6.3 GLYCINE-SERINE PEPTIDES 33
2.6.4 RATES OF CHEMICAL REACTIONS 33
3
MATERIALS AND METHODS
35
3.1 ENSEMBLE SPECTROMETER
35
3.2 FLUORESCENCE CORRELATION
SPECTROSCOPY SETUP 37
3.3 SAMPLE PREPARATION 40
4
REVEALING KINETICS OF OLIGOPEPTIDES USING
FCS
45
5
DYNAMICS OF GLYCOPEPTIDES
49
5.1 SAMPLE CHARACTERIZATION
49
5.2 DISCUSSION 55
6 PEPTIDE DYNAMICS MONITORED BY DIMERIZA-
TION
59
6.1 RESULTS 59
6.2 DISCUSSION 67
7
HOW FUNCTION OF HETERODIMERS INFLUENCES
FLUORESCENCE
73
7.1 HOMODIMERIZATION 74
7.1.1 MEASUREMENTS
AND RESULTS 74
7.1.2 DISCUSSION 80
7.2 INTRAMOLECULAR DIMERIZATION BETWEEN DIFFERENT DYES 83
7.2.1 MEASUREMENTS AND
RESULTS 83
7.2.2 DISCUSSION 90
8
CONCLUSION AND OUTLOOK
93
9
APPENDIX
99
9.1 MULTIPOLE
EXPANSION 99
IX
X
CONTENTS
9.2 POLARIZABILITY OF SINGLY DIPOLES 99
9.3 POLARIZATION OF AN ENSEMBLE OF FREE DIPOLES 100
9.4 CORRELATION FUNCTION FOR TWO-DIMENSIONAL DIFFUSION WITH
ISOMERIZATION 100
9.5 QUANTUM YIELDS OF ORGANIC DYES 104
|
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| spelling | Bollmann, Stefan Verfasser (DE-588)1067413375 aut Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes von Stefan Bollmann Bestimmung struktureller Dynamiken von Oligopeptiden mittels Fluoreszenzlöschung von organischen Fluorophoren 2013 XIII, 115 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache. - Online erschienen 2014 Würzburg, Univ., Diss., 2013 Fluorophore (DE-588)4334948-1 gnd rswk-swf Fluoreszenzlöschung (DE-588)4154827-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fluorophore (DE-588)4334948-1 s Fluoreszenzlöschung (DE-588)4154827-9 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-92191 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-92191 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027809901&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bollmann, Stefan Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes Fluorophore (DE-588)4334948-1 gnd Fluoreszenzlöschung (DE-588)4154827-9 gnd |
| subject_GND | (DE-588)4334948-1 (DE-588)4154827-9 (DE-588)4113937-9 |
| title | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes |
| title_alt | Bestimmung struktureller Dynamiken von Oligopeptiden mittels Fluoreszenzlöschung von organischen Fluorophoren |
| title_auth | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes |
| title_exact_search | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes |
| title_full | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes von Stefan Bollmann |
| title_fullStr | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes von Stefan Bollmann |
| title_full_unstemmed | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes von Stefan Bollmann |
| title_short | Structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes |
| title_sort | structural dynamics of oligopeptides determined by fluorescence quenching of organic dyes |
| topic | Fluorophore (DE-588)4334948-1 gnd Fluoreszenzlöschung (DE-588)4154827-9 gnd |
| topic_facet | Fluorophore Fluoreszenzlöschung Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-92191 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027809901&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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