Involvement of phagocytic cells in acute graft-versus-host disease:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 88 Bl. Ill., graph. Darst. 30 cm |
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Datensatz im Suchindex
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| adam_text | AUS DEM DEPARTMENT INNERE MEDIZIN
UNIVERSITATSKLINIKUM DER ALBERT-LUDWIGS-UNIVERSITAT FREIBURG I. BR.
KLINIKFUR INNERE MEDIZIN I
(SCHWERPUNKT: HAMATOLOGIE, ONKOLOGIE UND STAMMZELLTRANSPLANTATION)
INVOLVEMENT OF PHAGOCYTIC CELLS
IN ACUTE GRAFT-VERSUS-HOST DISEASE
INAUGURAL-DISSERTATION
ZUR ERLANGUNG DES MEDIZINISCHEN DOKTORGRADES
DER MEDIZINISCHEN FAKULTAT DER ALBERT-LUDWIGS-UNIVERSITAT FREIBURG I.
BR.
VORGELEGT 2012
VON LUKAS MARTIN SCHWAB
GEBOREN IN OSNABRIICK
HTTP://D-NB.INFO/1065827555
TABLE OF CONTENTS
1 INTRODUCTION 7
1.1 ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION 7
1.2 GVHD 8
1.3 MUCOSAL IMMUNITY IN GVHD 11
1.4 MURINE INFLAMMATORY MONOCYTES 14
1.5 AIMS OF THIS STUDY 15
2 MATERIALS 17
2.1 MOUSE STRAINS 17
2.2 MEDIA FOR CELL CULTURE AND SERA 20
2.3 CHEMICALS 20
2.4 ANTIBODIES 21
2.5 INSTRUMENTS 23
2.6 CONSUMABLES 24
2.7 REACTION KITS 25
2.8 PREPARATION OF READY-FOR-USE SOLUTIONS 25
2.9 SOFTWARE PROGRAMS 27
3 METHODS 28
3.1 ALLOGENEIC BONE MARROW TRANSPLANTATION (ALLO-BMT) AND GRAFT-VERSUS-
HOST DISEASE
MODEL 28
3.2 CREATION OF CCR2
/_
/ WT BM CHIMERIC MICE AND SUBSEQUENT ALLO-BMT 32
3.3 BIOLUMINESCENCE IMAGING 33
3.4 ANIMAL TREATMENT 36
3.5 COLLECTION OF PERIPHERAL VEIN BLOOD FOR FACS ANALYSIS
OF BLOOD LEUKOCYTE SUBSETS 37
3.6 ENZYMATIC DIGESTION OF THE SMALL INTESTINE FOR FACS ANALYSIS OF
INTESTINAL LEUKOCYTE
SUBSETS 37
3.7 FLOW CYTOMETRY 39
3.8 MEASUREMENT OF RNA EXPRESSION LEVELS IN THE TERMINAL ILEUM 40
3.9 HISTOLOGY PREPARATIONS 42
3.10 STATISTICAL ANALYSIS 44
4 RESULTS 45
4.1 IN VIVO MPO IMAGING REVEALS RISEN MPO ACTIVITY PROJECTING OVER THE
ABDOMINAL
AREA EARLY AFTER ALLO-BMT 45
4.2 THE SMALL INTESTINE AND THE COLON ARE THE SITES OF RISEN MPO
ACTIVITY OBSERVED
EARLY AFTER ALLO-BMT 47
4.3 GR-1
+
CELLS ACCUMULATE IN THE TERMINAL ILEUM EARLY AFTER ALLO-BMT 48
4.4 RAG-2 COMMON Y CHAIN
7
MICE SHOW INCREASED MPO ACTIVITY IN THE ABDOMINAL
REGION AFTER ALLO-BMT ONLY WHEN PREVIOUSLY IRRADIATED 49
4.5 IRRADIATION ALONE IS SUFFICIENT FOR INDUCTION OF INCREASED ABDOMINAL
MPO ACTIVITY IN
BALB/C WT MICE 51
4.6 MYELOABLATIVE IRRADIATION INDUCES A RELATIVE INCREASE OF PHAGOCYTIC
CELLS IN THE
SMALL INTESTINE 52
4.7 EARLY DEPLETION OF NEUTROPHILS IMPROVES THE SURVIVAL AFTER ALLO-BMT
53
4.8 EARLY DEPLETION OF CCR2
+
,
INFLAMMATORY MONOCYTES IN BALB/C WT RECIPIENTS
CORRELATES WITH A BETTER SURVIVAL AFTER ALLO-BMT 55
4.9 EARLY DEPLETION OF INFLAMMATORY MONOCYTES IN RAG-2 , COMMON Y
CHAIN
7
MICE
CORRELATES WITH A BETTER SURVIVAL AFTER ALLO-BMT 56
4.10 CCR2 DEFICIENT MICE HAVE LESS GVHD 59
4.11 CCR2
+
HEMATOPOIETIC CELLS NEGATIVELY AFFECT THE OUTCOME OF GVHD 60
4.12 GPGIPHOX
7
MICE SHOW A BETTER SURVIVAL THAN WT RECIPIENTS AFTER ALIO- BMT 63
4.13 MICROARRAY-BASED ANALYSES REVEAL AN UPREGULATION OF THE G-CSFR GENE
AND SEVERAL
CHEMOKINE GENES IN THE TERMINAL ILEUM AFTER IRRADIATION 65
5 DISCUSSION 68
6 SUMMARY 75
7 ZUSAMMENFASSUNG 76
8 ABBREVIATIONS 77
9 REFERENCES 81
10 ACKNOWLEDGEMENT / DANKSAGUNG
88
|
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| spelling | Schwab, Lukas Martin Verfasser (DE-588)1064718779 aut Involvement of phagocytic cells in acute graft-versus-host disease vorgelegt von Lukas Martin Schwab 2012 88 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027799929&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schwab, Lukas Martin Involvement of phagocytic cells in acute graft-versus-host disease |
| subject_GND | (DE-588)4113937-9 |
| title | Involvement of phagocytic cells in acute graft-versus-host disease |
| title_auth | Involvement of phagocytic cells in acute graft-versus-host disease |
| title_exact_search | Involvement of phagocytic cells in acute graft-versus-host disease |
| title_full | Involvement of phagocytic cells in acute graft-versus-host disease vorgelegt von Lukas Martin Schwab |
| title_fullStr | Involvement of phagocytic cells in acute graft-versus-host disease vorgelegt von Lukas Martin Schwab |
| title_full_unstemmed | Involvement of phagocytic cells in acute graft-versus-host disease vorgelegt von Lukas Martin Schwab |
| title_short | Involvement of phagocytic cells in acute graft-versus-host disease |
| title_sort | involvement of phagocytic cells in acute graft versus host disease |
| topic_facet | Hochschulschrift |
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