Assessing particle deposition in a representative in vitro model of the rat respiratory tract:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache |
| Beschreibung: | 300 Bl. graph. Darst. |
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| 245 | 1 | 0 | |a Assessing particle deposition in a representative in vitro model of the rat respiratory tract |c von Arabe Ahmed |
| 246 | 1 | 3 | |a Entwicklung eines in vitro Modells (IVR) der Rattenlunge für die Untersuchung der Deposition von Wirkstoffpartikeln in den Atemwegen der Ratte |
| 264 | 1 | |c 2014 | |
| 300 | |a 300 Bl. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Würzburg, Univ., Diss., 2014 | ||
| 650 | 0 | 7 | |a Wirkstoff |0 (DE-588)4066383-8 |2 gnd |9 rswk-swf |
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| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-104912 |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-027756315 | ||
Datensatz im Suchindex
| _version_ | 1804152917188411392 |
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| adam_text | ASSESSING
PARTICLE DEPOSITION IN A REPRESENTATIVE
IN VITRO
MODEL OF THE RAT RESPIRATORY TRACT
JULIUS-MAXIMILIANS-
UNIVERSITAT
WURZBURG
DISSERTATION ZUR ERLANGUNG DES NATURWISSENSCHAFTLICHEN DOKTORGRADES DER
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG
VORGELEGT VON
ARABE AHMED
AUS BAGHDAD, IRAQ
WURZBURG 2014
ZUR ERLANGUNG
DES NATURWISSENSCHAFTLICHEN DOKTORGRADES
DER JULIUS - MAXIMILIANS - UNIVERSITAT WURZBURG
HTTP://D-NB.INFO/1066776164
TABLE OF CONTENTS
A. INTRODUCTION 17
1 GENERAL INTRODUCTION 18
1.1 DEPOSITION OF PARTICLES IN THE LUNG 20
1.1.1 AEROSOL PARTICLE CHARACTERISTICS 20
1.1.2 THE AIRFLOW CONDITIONS 25
1.2 RESPIRATORY TRACT ANATOMY 26
1.2.1 HUMAN RESPIRATORY TRACT 26
1.2.2 RAT RESPIRATORY TRACT 31
1.3 DOSIMETRY IN INHALED DRUG DELIVERY 34
1.4 METHODS TO ASSESS DRUG DEPOSITION IN PRECLINICAL MODELS 36
1.4.1 PHARMACOKINETIC METHODS 36
1.4.2 LUNG HOMOGENATE METHOD 37
1.5 MODELLING OF AEROSOL DEPOSITION IN LUNGS 40
1.5.1 IN VITRO
MODELS 41
1.5.2 JN SILICO
MODELS 44
1.5.3 VALIDATION OF IN SILICO
LUNG DEPOSITION MODELS 49
1.6 IMAGING TECHNIQUES 50
1.7 INHALATION EXPOSURE STUDIES 52
1.7.1 DESIGN, METHOD AND CHARACTERISATION OF AEROSOL EXPOSURE SYSTEMS 53
1.7.2 GENERATION OF AEROSOLS 55
1.8 EXPOSURE ATMOSPHERE CHARACTERISATION 60
2 AIM OF THE THESIS 62
B. RESULTS AND DISCUSSION 63
1 DEVELOPMENT OF AEROSOL DELIVERY METHOD 64
1.1 METHOD USED FOR GENERATION OF AEROSOL POWDER FOR INHALATION 66
1.2 GENERATOR PERFORMANCE EVALUATION 68
1.3 SCREENING STUDY DETAILS 69
1.3.1 SCREENING STUDY RESULTS 71
1.3.2 MASS PARTICLE SIZE CONCENTRATION (MG/M
3
) 72
1.3.3 MASS MEDIAN AERODYNAMIC DIAMETER (NM) 73
1.4 DELIVERY METHOD EVALUATION 76
TABLE OF CONTENTS
1.4.1 EXPERIMENT USING 4 MICRON CONTAINING FMS BLENDS 81
1.5 VALIDATION OF THE USE OF THE APS 3321 FOR USE IN RODENT INHALATION
STUDIES 83
1.5.1 APS VERSUS GRAVIMETRIC CORRELATION 84
1.5.2 APS VERSUS HPLC CORRELATION 87
1.5.3 APS VERSUS MARPLE CASCADE IMPACTOR CORRELATION 89
1.6 CONCLUSION 91
2 VALIDATION OF NOSE-ONLY EXPOSURE SYSTEM FOR RODENTS 92
2.1 METHOD USED TOR CHARACTERISATION OF CHAMBER ATMOSPHERE AND ITS
PARTICLE SIZE
DISTRIBUTION 94
2.2 MODIFICATION OF EXPOSURE CHAMBER 96
2.3 ASSESSING THE VARIABILITY OF TOTAL PARTICULATE MATTER (TPM) IN THE
EXPOSURE CHAMBER 97
2.4 ASSESSING THE VARIABILITY OF ACTIVE TEST MATERIAL IN THE EXPOSURE
CHAMBER 98
2.5 ENVIRONMENTAL CONDITIONS WITHIN EXPOSURE CHAMBER 101
2.6 CHARACTERISATION OF EXPOSURE ATMOSPHERE 103
2.7 CONCLUSION 105
3 DEVELOPMENT OF THE IN VITRO RAT LUNG MODEL 106
3.1 CONDUCT OF WORK 107
3.2 MICRO-CT SCANNING OF THE RAT RESPIRATORY SYSTEM 107
3.3 PHYSICAL MODEL DETAILS 108
3.4 ASSESSING THE NUMBER OF AIRWAYS PER GENERATION FOR THE IVR MODEL 110
3.5 IVR MODEL TESTING SETUP 112
3.6 DEVELOPMENT OF COATING SOLUTION FOR THE IVR MODEL 113
4 ASSESSING THE EFFECT
OF AEROSOL CHARACTERISTICS ON DEPOSITION IN THE RAT MODEL.... 114
4.1 ASSESSING THE INFLUENCE OF INHALED PARTICLE SIZE ON REGIONAL LUNG
DEPOSITION IN THE IN
VITRO RAT MODEL 115
4.2 ASSESSING THE INFLUENCE OF DOSE INCREASE AND FORMULATION CHANGES ON
THE TOTAL AND
REGIONAL DEPOSITION OF FMS PARTICLES ON THE IVR MODEL 120
4.3 CONCLUSION 124
5 ASSESSING THE
EFFECT OF VENTILATION PARAMETERS ON DEPOSITION IN THE RAT MODEL 125
5.1 STUDY DESIGN 126
TABLE OF CONTENTS
5.2 AEROSOL GENERATION AND PARTICLE SIZE ANALYSIS 127
5.3 INFLUENCE OF VENTILATION PARAMETERS ON LUNG DEPOSITION IN THE IVR
MODEL 129
5.4 TOTAL DEPOSITION IN THE IVR MODEL COMPARED TO THE MPPD MODEL
PREDICTIONS 132
5.5 REGIONAL DEPOSITION IN THE IVR MODEL COMPARED TO THE MPPD MODEL
PREDICTIONS 134
5.6 DEPOSITION IN THE IVR MODEL COMPARED TO IN VIVO
DATA FROM LITERATURE 136
5.7 IMPACT OF POLYDISPERSITY OF AEROSOLISED FMS PARTICLE ON THE
VARIABILITY IN
EXPERIMENTAL DATA 138
5.8 LIMITATION OF THE IVR MODEL 139
5.9 CONCLUSIONS 140
6 COMPARATIVE DEPOSITION OF INHALED AEROSOLS IN EXPERIMENTAL RATS AND IN
VITRO
RAT
LUNG MODEL 141
6.1 STUDY DESIGN 143
6.2 TOTAL DEPOSITION IN THE IVR MODEL COMPARED TO ESTIMATES OF TOTAL
DEPOSITION BASED
ON FILTER SAMPLES 144
6.3 LUNG REGION COMPARISON BETWEEN IVR MODEL, LIVE ANIMALS AND IN SILICO
MPPD
MODEL 146
6.3.1 COMPARATIVE LUNG DEPOSITION 148
6.3.1.1 LUNG DEPOSITION IN THE IVR MODEL VERSUS IN VIVO
LUNG HOMOGENATE DATA 148
6.3.1.2 FRACTIONAL LUNG DEPOSITION COMPARISON IN THE IVR MODEL VERSUS IN
VIVO
LUNG
HOMOGENATE DATA VERSUS IN SILICO MPPD ESTIMATES 151
6.4 DEPOSITION MEASUREMENTS IN THE IVR MODEL VERSUS IN SILICO
MPPD MODELLING
ESTIMATES 153
6.5 ASSESSING THE EFFECT OF FORMULATION CHARACTERISTICS ON REGIONAL
DEPOSITION OF INHALED
PARTICLES IN THE IVR MODEL VERSUS IN VIVO LUNG HOMOGENATE DATA 158
6.5.1 COMBINING DEPOSITION DATA FROM ALL STUDIES TO ASSESS THE EFFECT OF
MMAD ON LUNG
DEPOSITION 162
6.6 CONCLUSIONS 165
7 ASSESSING THE RELATIONSHIP BETWEEN EFFICACY OF INHALED FLUTICASONE
PROPIONATE
IN AN
ALLERGEN-INDUCED RODENT MODEL WITH REGIONAL LUNG DEPOSITION 167
7.1 STUDY DESIGN 170
7.2 RESULTS AND DISCUSSION 172
7.3 CONCLUSION 177
C. EXPERIMENTAL SETUP 178
TABLE OF CONTENTS
1 DEVELOPMENT OF AEROSOL DELIVERY METHOD 179
1.1 MATERIALS AND EQUIPMENT 179
1.1.1 MATERIALS 179
1.1.1 180
1.1.2 EQUIPMENT 181
1.1.3 HPLC QUANTIFICATION OF FLUORESCENT MICROSPHERES 186
1.1.4 DETERMINATION OF PARTICLE SIZE DISTRIBUTION USING MARPLE CASCADE
IMPACTOR (MCI) 188
1.1.5 STATISTICAL DATA ANALYSIS 188
1.2 AEROSOL GENERATION METHODS 189
1.2.1 SCREENING STUDY 189
1.2.2 DELIVERY METHOD EVALUATION 190
1.2.3 VALIDATION THE APS 3321 FOR USE IN RODENT INHALATION STUDIES 191
2 VALIDATION OF NOSE-ONLY EXPOSURE SYSTEM FOR RODENTS 193
2.1 MATERIALS AND EQUIPMENTS 193
2.1.1 MATERIALS 193
2.1.2 EQUIPMENTS 193
2.2 METHODS 193
2.2.1 EXPOSURE TECHNIQUE AND GENERATION OF AEROSOL 193
2.2.2 AEROSOL MONITORING AND EVALUATION 195
2.2.3 EXPERIMENTAL PROCEDURE FOR OPERATING THE EXPOSURE SYSTEM 196
3 ASSESSING THE EFFECT OF AEROSOL
CHARACTERISTICS ON DEPOSITION IN THE
RAT MODEL.... 198
3.1 MATERIALS AND EQUIPMENTS 198
3.1.1 MATERIALS 198
3.1.2 EQUIPMENT 199
3.2 AEROSOL GENERATION AND CHARACTERISATION OF CHAMBER ATMOSPHERES 200
3.3 VENTILATION PARAMETERS MONITORING 202
3.4 DOSE ESTIMATION USING MULTIPLE-PATH PARTICLE DOSIMETERY MODEL (MPPD)
204
4 ASSESSING THE EFFECT
OF VENTILATION PARAMETERS ON DEPOSITION IN THE RAT MODEL 205
4.1 MATERIALS AND EQUIPMENTS 205
4.1.1 BLEND MANUFACTURE 205
4.1.2 EQUIPMENT 205
4.2 AEROSOL GENERATION AND CHARACTERISATION OF CHAMBER ATMOSPHERES 205
TABLE OF CONTENTS
4.3 IN VITRO RAT (IVR) MODEL DETAILS 205
4.4 VENTILATION PARAMETERS MONITORING 205
4.5 SAMPLE PREPARATION AND HPLC METHOD DETAILS 205
4.6 DOSE ESTIMATION USING MULTIPLE-PATH PARTICLE DOSIMETERY MODEL (MPPD)
206
4.7 DOSE ESTIMATION BASED ON FILTER SAMPLES 206
4.7.1 STATISTICAL DATA ANALYSIS 206
5 COMPARATIVE DEPOSITION OF INHALED AEROSOLS IN EXPERIMENTAL RATS AND IN
VITRO
RAT
LUNG MODEL 207
5.1 MATERIALS 207
5.2 ANIMAL CARE AND ETHICAL APPROVAL 207
5.3 CONDUCT OF EXPERIMENTAL PROCEDURE 207
5.4 METHOD OF AEROSOL GENERATION AND EXPOSURE SETUP 208
5.4.1 COMPOUND X AND Y 208
5.4.2 FLUTICASONE PROPIONATE (FP) 209
5.5 CHAMBER AEROSOL CHARACTERISATION 211
5.6 DOSE ESTIMATION 213
5.7 LUNG CONCENTRATION DETERMINATION FOR COMPOUND X AND Y 213
5.7.1 SAMPLE PREPARATION 213
5.7.2 SAMPLE EXTRACTION FOR COMPOUND X 213
5.7.3 SAMPLE EXTRACTION FOR COMPOUND Y 214
5.8 LUNG CONCENTRATION DETERMINATION FOR FLUTICASONE PROPIONATE 214
5.9 SAMPLE PREPARATION FOR THE IN VITRO (IVR) RAT MODEL DETAILS 217
5.10 DOSE ESTIMATION USING MULTIPLE-PATH PARTICLE DOSIMETERY MODEL
(MPPD) 217
5.11 STATISTICS 217
6 ASSESSING THE RELATIONSHIP BETWEEN EFFICACY OF INHALED FLUTICASONE
PROPIONATE
IN AN
ALLERGEN-INDUCED RODENT MODEL WITH REGIONAL LUNG DEPOSITION 218
6.1 CONDUCT OF EXPERIMENTAL PROCEDURE 218
6.2 ANIMALS USED IN STUDY 218
6.3 INHALATION METHODOLOGY 218
6.4 IN VIVO LUNG DOSE DETERMINATION 219
TABLE OF CONTENTS
6.4.1.1 SAMPLE PREPARATION 219
6.4.1.2 PREPARATION OF COMPOUND STANDARDS IN LUNG HOMOGENATE 219
6.4.1.3 SAMPLE EXTRACTION 220
6.5 IVR ANALYSIS 220
6.6 MPPD ANALYSIS 221
D. APPENDIX 223
1 DEVELOPMENT OF AEROSOL DELIVERY METHOD 224
1.1 TABLE 33: AEROSOL CONCENTRATION (MG/M
3
) INPUT INTO DOE MODEL (TABLE 5) 224
1.2 TABLE 34: MASS MEDIAN AERODYNAMIC DIAMETER ( XM) INPUT INTO DOE
MODEL (TABLE
5) 227
1.3 STATISTICAL ANALYSIS FOR SCREENING STUDY 230
1.3.1 AEROSOL CONCENTRATION 230
1.3.2 PARTICLE SIZE 230
1.4 TABLE 37: MASS MEDIAN AERODYNAMIC DIAMETER (|IM) DATA FOR FIGURE 14
231
1.5 TABLE 38: AEROSOL CONCENTRATION (MG/M
3
) DATA FOR FIGURE 15 236
1.6 TABLE 39: AEROSOL CONCENTRATION (MG/M
3
) DATA FOR FIGURE 16 238
1.7 TABLE 40: MASS MEDIAN AERODYNAMIC DIAMETER (PIM)
DATA FOR FIGURE 17 238
1.8 TABLE 41: AEROSOL CONCENTRATION (MG/M
3
) DATA FOR FIGURE 18 .239
1.9 TABLE 42: AEROSOL CONCENTRATION (MG/M
3
) DATA FOR FIGURE 19 240
1.10 TABLE 43: GRAVIMETRIC AND APS AEROSOL CONCENTRATION (MG/M
3
) DATA FOR FIGURE 20 242
1.11 TABLE 44: APS EVENT DATA FOR FIGURE 21 243
1.12 TABLE 45: APS VERSUS HPLC CORRELATION DATA FOR FIGURE 22 244
1.13 TABLE 46: MARPLE CASCADE IMPACTOR DATA USED FOR FIGURE 23 AND
FIGURE 24 245
1.14 TABLE 47: APS DATA FOR FIGURE 24 246
2 VALIDATION OF NOSE-ONLY EXPOSURE SYSTEM FOR RODENTS 248
2.1 TABLE 48: COMPARISON OF THE AEROSOL CONCENTRATION OF THE FMS-LACTOSE
BLENDS
BEFORE AND AFTER APPLICATION OF CHARGE NEUTRALISATION (SEE FIGURE 28)
248
2.2 TABLE 49: ASSESSING THE VARIABILITY OF TOTAL PARTICULATE MATTER
(TPM) IN THE EXPOSURE
CHAMBER (SEE FIGURE 29) 249
2.3 TABLE 50: ASSESSING THE VARIABILITY OF ACTIVE TEST MATERIAL IN THE
EXPOSURE CHAMBER
(SEE TABLE 7) 250
TABLE OF CONTENTS
2.4 TABLE 51: ENVIRONMENTAL CONDITIONS WITHIN EXPOSURE CHAMBER 251
2.5 TABLE 52: CHARACTERISATION OF EXPOSURE ATMOSPHERE 252
3 ASSESSING THE
EFFECT OF AEROSOL CHARACTERISTICS ON DEPOSITION IN THE RAT MODEL.... 254
3.1 CHARACTERISATION OF EXPOSURE ATMOSPHERE FOR TABLE 8 AND 9 254
3.2 IVR DATA FOR TABLE 8, FIGURE 42 AND 43 256
3.3 MPPD DATA FOR FIGURE 43 257
3.4 AEROSOL CONCENTRATION DATA FOR TABLE 10 258
3.5 IVR DEPOSITION DATA FOR TABLE 10 AND FIGURE 45 259
3.6 MARPLE PARTICLE SIZE DATA FOR TABLE 10 AND FIGURE 44 260
4 ASSESSING THE EFFECT
OF VENTILATION PARAMETERS ON DEPOSITION IN THE RAT MODEL 263
4.1 IVR DEPOSITION DATA FOR TABLE 11 263
4.2 MPPD DEPOSITION DATA FOR TABLE 12 265
4.3 STATISTICAL ANALYSIS FOR ASSESSING THE EFFECT OF VENTILATION
PARAMETERS ON DEPOSITION
IN THE RAT MODEL 267
4.3.1 HEAD REGION DEPOSITION (% OF TOTAL) 267
4.3.2 TB REGION DEPOSITION (% OF TOTAL) 267
4.3.3 POST-TB REGION DEPOSITION (% OF TOTAL) 268
4.3.4 HEAD REGION DEPOSITION (MEG) 269
5 COMPARATIVE DEPOSITION OF INHALED AEROSOLS IN EXPERIMENTAL RATS AND
IVR RAT
LUNG MODEL 270
5.1 TABLE 67: IN VIVO
LUNG HOMOGENATE DATA OF TOTAL DEPOSITION FOR COMPOUND Y,
COMPOUND X AND FLUTICASONE PROPIONATE, DATA LIST WAS USED TO CONSTRUCT
FIGURE 53
AND FIGURE 54 270
5.2 IVR DATA FOR COMPOUND Y TEST MATERIAL 272
5.3 IVR DATA FOR COMPOUND X TEST MATERIAL 273
5.4 IVR DATA FOR FLUTICASONE PROPIONATE 274
5.5 POOLED LUNG DEPOSITION DATA FOR FIGURE 58 275
6 ASSESSING THE RELATIONSHIP
BETWEEN EFFICACY OF INHALED FLUTICASONE IN AN
ALLERGEN-INDUCED RODENT MODEL WITH REGIONAL LUNG DEPOSITION 277
6.1 SUMMARY TABLE OF DATA USED IN SECTION 7, B- RESULTS AND DISCUSSION
277
E. SUMMARY 278
1. SUMMARY 279
TABLE OF CONTENTS
2. ZUSAMMENFASSUNG 282
F. ABBREVIATIONS 286
G. REFERENCES 288
|
| any_adam_object | 1 |
| author | Ahmed, Arabe |
| author_GND | (DE-588)1065919514 |
| author_facet | Ahmed, Arabe |
| author_role | aut |
| author_sort | Ahmed, Arabe |
| author_variant | a a aa |
| building | Verbundindex |
| bvnumber | BV042319355 |
| classification_rvk | VX 5070 |
| collection | ebook |
| ctrlnum | (OCoLC)902816827 (DE-599)BVBBV042319355 |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV042319355 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:18:19Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027756315 |
| oclc_num | 902816827 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 300 Bl. graph. Darst. |
| psigel | ebook |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| record_format | marc |
| spelling | Ahmed, Arabe Verfasser (DE-588)1065919514 aut Assessing particle deposition in a representative in vitro model of the rat respiratory tract von Arabe Ahmed Entwicklung eines in vitro Modells (IVR) der Rattenlunge für die Untersuchung der Deposition von Wirkstoffpartikeln in den Atemwegen der Ratte 2014 300 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2014 Wirkstoff (DE-588)4066383-8 gnd rswk-swf In vitro (DE-588)4250701-7 gnd rswk-swf Atemwege (DE-588)4068903-7 gnd rswk-swf Ratte (DE-588)4177011-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ratte (DE-588)4177011-0 s Atemwege (DE-588)4068903-7 s In vitro (DE-588)4250701-7 s Wirkstoff (DE-588)4066383-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-104912 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-104912 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027756315&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ahmed, Arabe Assessing particle deposition in a representative in vitro model of the rat respiratory tract Wirkstoff (DE-588)4066383-8 gnd In vitro (DE-588)4250701-7 gnd Atemwege (DE-588)4068903-7 gnd Ratte (DE-588)4177011-0 gnd |
| subject_GND | (DE-588)4066383-8 (DE-588)4250701-7 (DE-588)4068903-7 (DE-588)4177011-0 (DE-588)4113937-9 |
| title | Assessing particle deposition in a representative in vitro model of the rat respiratory tract |
| title_alt | Entwicklung eines in vitro Modells (IVR) der Rattenlunge für die Untersuchung der Deposition von Wirkstoffpartikeln in den Atemwegen der Ratte |
| title_auth | Assessing particle deposition in a representative in vitro model of the rat respiratory tract |
| title_exact_search | Assessing particle deposition in a representative in vitro model of the rat respiratory tract |
| title_full | Assessing particle deposition in a representative in vitro model of the rat respiratory tract von Arabe Ahmed |
| title_fullStr | Assessing particle deposition in a representative in vitro model of the rat respiratory tract von Arabe Ahmed |
| title_full_unstemmed | Assessing particle deposition in a representative in vitro model of the rat respiratory tract von Arabe Ahmed |
| title_short | Assessing particle deposition in a representative in vitro model of the rat respiratory tract |
| title_sort | assessing particle deposition in a representative in vitro model of the rat respiratory tract |
| topic | Wirkstoff (DE-588)4066383-8 gnd In vitro (DE-588)4250701-7 gnd Atemwege (DE-588)4068903-7 gnd Ratte (DE-588)4177011-0 gnd |
| topic_facet | Wirkstoff In vitro Atemwege Ratte Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-104912 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027756315&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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