Aqueous size-exclusion chromatography:
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier
1988
|
| Schriftenreihe: | Journal of chromatography library
v. 40 |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Beschreibung: | The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole. This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating Includes bibliographical references and index |
| Beschreibung: | 1 Online-Ressource (xviii, 453 p.) |
| ISBN: | 9780444429575 0444429573 |
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| 500 | |a The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole. This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating | ||
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| discipline | Chemie / Pharmazie |
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| isbn | 9780444429575 0444429573 |
| language | English |
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| spelling | Aqueous size-exclusion chromatography edited by P.L. Dubin Amsterdam Elsevier 1988 1 Online-Ressource (xviii, 453 p.) txt rdacontent c rdamedia cr rdacarrier Journal of chromatography library v. 40 The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole. This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating Includes bibliographical references and index Chromatographie sur gel Hydrofobe interactie chromatografie gtt Biopolymeren gtt Poreuze media gtt Silica gtt Gel permeation chromatography fast Gel permeation chromatography Gelchromatographie (DE-588)4156414-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Gelchromatographie (DE-588)4156414-5 s 2\p DE-604 Dubin, Paul Sonstige oth http://www.sciencedirect.com/science/book/9780444429575 Verlag Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Aqueous size-exclusion chromatography Chromatographie sur gel Hydrofobe interactie chromatografie gtt Biopolymeren gtt Poreuze media gtt Silica gtt Gel permeation chromatography fast Gel permeation chromatography Gelchromatographie (DE-588)4156414-5 gnd |
| subject_GND | (DE-588)4156414-5 (DE-588)4143413-4 |
| title | Aqueous size-exclusion chromatography |
| title_auth | Aqueous size-exclusion chromatography |
| title_exact_search | Aqueous size-exclusion chromatography |
| title_full | Aqueous size-exclusion chromatography edited by P.L. Dubin |
| title_fullStr | Aqueous size-exclusion chromatography edited by P.L. Dubin |
| title_full_unstemmed | Aqueous size-exclusion chromatography edited by P.L. Dubin |
| title_short | Aqueous size-exclusion chromatography |
| title_sort | aqueous size exclusion chromatography |
| topic | Chromatographie sur gel Hydrofobe interactie chromatografie gtt Biopolymeren gtt Poreuze media gtt Silica gtt Gel permeation chromatography fast Gel permeation chromatography Gelchromatographie (DE-588)4156414-5 gnd |
| topic_facet | Chromatographie sur gel Hydrofobe interactie chromatografie Biopolymeren Poreuze media Silica Gel permeation chromatography Gelchromatographie Aufsatzsammlung |
| url | http://www.sciencedirect.com/science/book/9780444429575 |
| work_keys_str_mv | AT dubinpaul aqueoussizeexclusionchromatography |