Nitric oxide synthase: characterization and functional analysis
Gespeichert in:
Bibliographische Detailangaben
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: San Diego Academic Press 1996
Schriftenreihe:Methods in neurosciences v. 31
Schlagworte:
Online-Zugang:Volltext
Beschreibung:Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field. Key Features * Comprehensive protocols included for * Detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques * Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression * Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone * Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution
Includes bibliographical references and index
Beschreibung:1 Online-Ressource (xviii, 354 p.)
ISBN:9780121853013
0121853012
9780080536378
0080536379

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