Molecular protocols in transfusion medicine:
Gespeichert in:
1. Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
San Diego, CA
Academic Press
2000
|
Schlagworte: | |
Online-Zugang: | Volltext |
Beschreibung: | This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests) Includes bibliographical references and index |
Beschreibung: | 1 Online-Ressource (x, 186 p.) |
ISBN: | 9780122093708 0122093704 9781435608092 1435608097 0080536905 9780080536903 128103276X 9781281032768 |
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500 | |a This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests) | ||
500 | |a Includes bibliographical references and index | ||
650 | 7 | |a Handbooks, manuals, etc |2 fast | |
650 | 4 | |a Blood Grouping and Crossmatching / Laboratory Manuals | |
650 | 4 | |a Blood Transfusion / Laboratory Manuals | |
650 | 4 | |a Polymorphism, Genetic / Laboratory Manuals | |
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650 | 7 | |a Genetic polymorphisms |2 fast | |
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650 | 4 | |a Genetic polymorphisms |v Handbooks, manuals, etc | |
650 | 4 | |a Blood |x Transfusion |v Handbooks, manuals, etc | |
700 | 1 | |a Rios, Maria |e Sonstige |4 oth | |
700 | 1 | |a Reid, Marion E. |e Sonstige |4 oth | |
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Datensatz im Suchindex
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any_adam_object | |
author | Denomme, Gregory A. |
author_facet | Denomme, Gregory A. |
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author_sort | Denomme, Gregory A. |
author_variant | g a d ga gad |
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dewey-full | 612.1/1825 |
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dewey-ones | 612 - Human physiology |
dewey-raw | 612.1/1825 |
dewey-search | 612.1/1825 |
dewey-sort | 3612.1 41825 |
dewey-tens | 610 - Medicine and health |
discipline | Medizin |
format | Electronic eBook |
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spelling | Denomme, Gregory A. Verfasser aut Molecular protocols in transfusion medicine Gregory A. Denomme, Maria Rios, Marion E. Reid San Diego, CA Academic Press 2000 1 Online-Ressource (x, 186 p.) txt rdacontent c rdamedia cr rdacarrier This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests) Includes bibliographical references and index Handbooks, manuals, etc fast Blood Grouping and Crossmatching / Laboratory Manuals Blood Transfusion / Laboratory Manuals Polymorphism, Genetic / Laboratory Manuals Hematology MEDICAL / Physiology bisacsh SCIENCE / Life Sciences / Human Anatomy & Physiology bisacsh Blood groups fast Blood / Transfusion fast Genetic polymorphisms fast Medizin Blood groups Handbooks, manuals, etc Genetic polymorphisms Handbooks, manuals, etc Blood Transfusion Handbooks, manuals, etc Rios, Maria Sonstige oth Reid, Marion E. Sonstige oth http://www.sciencedirect.com/science/book/9780122093708 Verlag Volltext |
spellingShingle | Denomme, Gregory A. Molecular protocols in transfusion medicine Handbooks, manuals, etc fast Blood Grouping and Crossmatching / Laboratory Manuals Blood Transfusion / Laboratory Manuals Polymorphism, Genetic / Laboratory Manuals Hematology MEDICAL / Physiology bisacsh SCIENCE / Life Sciences / Human Anatomy & Physiology bisacsh Blood groups fast Blood / Transfusion fast Genetic polymorphisms fast Medizin Blood groups Handbooks, manuals, etc Genetic polymorphisms Handbooks, manuals, etc Blood Transfusion Handbooks, manuals, etc |
title | Molecular protocols in transfusion medicine |
title_auth | Molecular protocols in transfusion medicine |
title_exact_search | Molecular protocols in transfusion medicine |
title_full | Molecular protocols in transfusion medicine Gregory A. Denomme, Maria Rios, Marion E. Reid |
title_fullStr | Molecular protocols in transfusion medicine Gregory A. Denomme, Maria Rios, Marion E. Reid |
title_full_unstemmed | Molecular protocols in transfusion medicine Gregory A. Denomme, Maria Rios, Marion E. Reid |
title_short | Molecular protocols in transfusion medicine |
title_sort | molecular protocols in transfusion medicine |
topic | Handbooks, manuals, etc fast Blood Grouping and Crossmatching / Laboratory Manuals Blood Transfusion / Laboratory Manuals Polymorphism, Genetic / Laboratory Manuals Hematology MEDICAL / Physiology bisacsh SCIENCE / Life Sciences / Human Anatomy & Physiology bisacsh Blood groups fast Blood / Transfusion fast Genetic polymorphisms fast Medizin Blood groups Handbooks, manuals, etc Genetic polymorphisms Handbooks, manuals, etc Blood Transfusion Handbooks, manuals, etc |
topic_facet | Handbooks, manuals, etc Blood Grouping and Crossmatching / Laboratory Manuals Blood Transfusion / Laboratory Manuals Polymorphism, Genetic / Laboratory Manuals Hematology MEDICAL / Physiology SCIENCE / Life Sciences / Human Anatomy & Physiology Blood groups Blood / Transfusion Genetic polymorphisms Medizin Blood groups Handbooks, manuals, etc Genetic polymorphisms Handbooks, manuals, etc Blood Transfusion Handbooks, manuals, etc |
url | http://www.sciencedirect.com/science/book/9780122093708 |
work_keys_str_mv | AT denommegregorya molecularprotocolsintransfusionmedicine AT riosmaria molecularprotocolsintransfusionmedicine AT reidmarione molecularprotocolsintransfusionmedicine |