Membrane protein purification and crystallization: a practical guide
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Academic Press
c2003
|
| Ausgabe: | 2nd ed |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Beschreibung: | This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins Includes bibliographical references and index |
| Beschreibung: | 1 Online-Ressource (xviii, 316 p.) |
| ISBN: | 9780123617767 0123617766 9780080536170 0080536174 |
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Datensatz im Suchindex
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| id | DE-604.BV042305390 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T01:17:52Z |
| institution | BVB |
| isbn | 9780123617767 0123617766 9780080536170 0080536174 |
| language | English |
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| physical | 1 Online-Ressource (xviii, 316 p.) |
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| publisher | Academic Press |
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| spelling | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger 2nd ed Amsterdam Academic Press c2003 1 Online-Ressource (xviii, 316 p.) txt rdacontent c rdamedia cr rdacarrier This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins Includes bibliographical references and index SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Proteínas larpcal Membranas (biologia) larpcal Bioquímica larpcal Membrane proteins Purification Membranproteine (DE-588)4130026-9 gnd rswk-swf Kristallisation (DE-588)4033215-9 gnd rswk-swf Reinigungsverfahren (DE-588)4391913-3 gnd rswk-swf Electronic books Membranproteine (DE-588)4130026-9 s Reinigungsverfahren (DE-588)4391913-3 s 1\p DE-604 Kristallisation (DE-588)4033215-9 s 2\p DE-604 Jagow, Gebhard von Sonstige oth Schägger, Hermann Sonstige oth http://www.sciencedirect.com/science/book/9780123617767 Verlag Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Membrane protein purification and crystallization a practical guide SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Proteínas larpcal Membranas (biologia) larpcal Bioquímica larpcal Membrane proteins Purification Membranproteine (DE-588)4130026-9 gnd Kristallisation (DE-588)4033215-9 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| subject_GND | (DE-588)4130026-9 (DE-588)4033215-9 (DE-588)4391913-3 |
| title | Membrane protein purification and crystallization a practical guide |
| title_auth | Membrane protein purification and crystallization a practical guide |
| title_exact_search | Membrane protein purification and crystallization a practical guide |
| title_full | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_fullStr | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_full_unstemmed | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_short | Membrane protein purification and crystallization |
| title_sort | membrane protein purification and crystallization a practical guide |
| title_sub | a practical guide |
| topic | SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Proteínas larpcal Membranas (biologia) larpcal Bioquímica larpcal Membrane proteins Purification Membranproteine (DE-588)4130026-9 gnd Kristallisation (DE-588)4033215-9 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| topic_facet | SCIENCE / Life Sciences / Anatomy & Physiology SCIENCE / Life Sciences / Biochemistry Proteínas Membranas (biologia) Bioquímica Membrane proteins Purification Membranproteine Kristallisation Reinigungsverfahren |
| url | http://www.sciencedirect.com/science/book/9780123617767 |
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