A practical guide to protein and peptide purification for microsequencing:
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Bibliographische Detailangaben
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: San Diego Academic Press c1993
Ausgabe:2nd ed
Schlagworte:
Online-Zugang:FAW01
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Beschreibung:Includes bibliographical references (p. 159-184)
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition:. Mass spectrophotometric characterization of proteins and peptides. N-terminal sequencing of proteins with blocked N termini. Internal amino acid sequence analysis after protease digestion in-gel and on-blot. Improved microscale peptide purification methods. Computer analysis of protein sequences. New protocols tested and refined through everyday use in authors' laboratories. Updated reference chapter covering all aspects of protein microsequencing
Beschreibung:1 Online-Ressource (xx, 184 p.)
ISBN:0080924611
0124802826
9780080924618
9780124802827

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