The cyclin dependent kinase 16: regulation, interactions, substrates and physiological function
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 145 S. Ill., graph. Darst. 21 cm |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
ABSTRACT 1
ZUSAMMENFASSUNG 2
INTRODUCTION 3
POST-TRANSLATIONAL MODIFICATIONS 4
PROTEIN KINASES 6
THE CDK PROTEIN KINASE FAMILY 10
CDK ACTIVATING CO-FACTORS - CYCLINS 13
THE CELL CYCLE - A TIGHTLY REGULATED PROCESS 14
PCTAIRE PROTEIN KINASES 17
CDK
16 AND CYCLIN Y 20
THE CYCLIN-DEPENDENT KINASE INHIBITOR P27
KLPL
32
INSULIN SIGNALING 39
AIMS OF THIS STUDY 44
RESULTS & DISCUSSION 45
INTERACTIONS OF CDK 16, CCN Y AND P27 46
SUBCELLULAR LOCALIZATION 51
CATALYTIC ACTIVITY AND PHYSIOLOGICAL FUNCTION 57
REGULATION OF CDK16 S CATALYTIC ACTIVITY 57
COLONY FORMATION ASSAYS 62
IDENTIFICATION OF NOVEL CDK 16 INTERACTION PARTNERS 67
IDENTIFICATION OF NOVEL CDK16 SUBSTRATES 71
PROTOARRAY HUMAN PROTEIN MICROARRAY 71
IDENTIFICATION OF P27 AS A NOVEL CDK 16 SUBSTRATE.. 77
MAPPING OF PHOSPHORYLATION SITES - PEPTIDE ARRAY 80
CDK 16 PHOSPHORY LAICS TUG ON S500 84
CDK16 AND TUG IN INSULIN SIGNALING 87
CONCLUSIONS & PERSPECTIVES 97
EXPERIMENTAL PROCEDURES 101
WORK WITH NUCLEIC ACIDS 102
CONVENTIONAL CLONING 102
GATEWAY CLONING 103
SITC-DIRCCTCD MUTAGENESIS 103
PSUPCR SYSTEM 103
WORK WITH PROKARYOTIC CELLS 104
BACTERIAL TRANSFORMATION 104
BACTERIAL CELL CULTIVATION 104
PLASMID PREPARATION 104
PURIFICATION OF GST-FUSION PROTEINS. . . 105
PURIFICATION OF HIS-TAGGCD PROTEINS.. 106
WORK WITH EUKARYOTIC CELLS 107
TISSUE CULTURE 107
TRANSIENT TRANSFCCLION 107
GENERATION OF STABLE CELL LINES 108
PREPARATION OF CELL LYSATES 108
HTTP://D-NB.INFO/1055055711
WORK WITH PROTEINS 110
SDS-PAGE 110
RAPID COOMASSIC STAINING 110
WESTERN BLOT 110
IMMUNODETECTION OF PROTEINS 110
CO-IMMUNOPRCCIPITATION 111
TANDEM AFFINITY PURIFICATION 111
SUBCELLULAR FRACTIONATION.... 112
IN VITRO ASSAYS 113
KINASE ASSAY 113
IN VITRO TRANSCRIPTION/TRANSLATION 113
GST PULL-DOWN ASSAY 113
HIS PULL-DOWN ASSAY 114
PROTOARRAYOP 114
PEPTIDE ARRAY 115
CELL-BASED ASSAYS 116
COLONY FORMATION ASSAY 116
IMMUNOFLUORCSCCNT STAINING OF ADHERENT CELLS 117
FLUORESCENCE ACTIVATED CELL SORTING (FACS) 117
REFERENCES 119
APPENDIX 132
ABBREVIATIONS 132
MATERIALS 135
OLIGONUCLEOTIDES 135
PLASMIDS 136
ANTIBODIES 138
BACTERIAL STRAINS 138
EUKARYOTIC CELL LINES 139
EIDCSSTATTLICHE ERKLSRUNG 143
CURRICULUM VITAE 144
DANKSAGUNG 145
|
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| dewey-full | 571.843742364 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.843742364 |
| dewey-search | 571.843742364 |
| dewey-sort | 3571.843742364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| language | English |
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| spelling | Agalaridis, Georgios 1982- Verfasser (DE-588)1051602130 aut The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function vorgelegt von Georgios Agalaridis 2014 IV, 145 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Aachen, Techn. Hochsch., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729711&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Agalaridis, Georgios 1982- The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function |
| subject_GND | (DE-588)4113937-9 |
| title | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function |
| title_auth | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function |
| title_exact_search | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function |
| title_full | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function vorgelegt von Georgios Agalaridis |
| title_fullStr | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function vorgelegt von Georgios Agalaridis |
| title_full_unstemmed | The cyclin dependent kinase 16 regulation, interactions, substrates and physiological function vorgelegt von Georgios Agalaridis |
| title_short | The cyclin dependent kinase 16 |
| title_sort | the cyclin dependent kinase 16 regulation interactions substrates and physiological function |
| title_sub | regulation, interactions, substrates and physiological function |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729711&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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