Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity:
Gespeichert in:
1. Verfasser: | |
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
2014
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Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | 119 S. graph. Darst. |
Internformat
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Datensatz im Suchindex
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adam_text | TABLE OF CONTENTS
1 INTRODUCTION 1
1.1 INFLAMMATION 4
1.2 NLR FAMILY AND INFLAMMASOMES 2
1.2.1 THE NLRP3 INFLAMMASOME: STRUCTURE, FUNCTION AND ACTIVATION 3
1.3 NLRP3 INFLAMMASOME AND METABOLIC DISORDERS 8
1.3.1 NLRP3 INFLAMMASOME AND ATHEROSCLEROSIS IO
1.3.2 NLRP3 INFLAMMASOME AND TYPE 2 DIABETES (T2D) IO
1.3.3 NLRP3 INFLAMMASOME AND ALZHEIMER S DISEASE (AD) 13
1.4 EFFECTS OF AMYLOID POLYPEPTIDES ON NLRP3 INFLAMMASOME ACTIVATION 14
1.4.1 AMYLOID POLYPEPTIDES, PROPERTIES AND FIBRILOGENESIS 14
1.4.2 BIOLOGY OF (5-AMYLOID-PEPTIDE PRODUCTION AND ITS ROLE IN
PATHOGENESIS OF ALZHEIMER S
DISEASE (AD) IS
1.4.3 HUMAN ISLET AMYLOID POLYPEPTIDE (HIAPP) AND PATHOGENESIS OF TYPE 2
DIABETES MELLITUS
(T2DM) __ IS
1.5 INHIBITORY EFFECTS OF IAPP MIMIC, IAPP-GI, ON FIBRILOGENESIS 19
1.6 THE AMYLIN RECEPTORS
21
1.7 AIM OF THE STUDY 23
2 MATERIALS AND METHODS 24
2.1 MATERIALS 24
2.1.1 CHEMICALS 24
2.1.2 ASSAY KITS 26
2.1.3 MATERIALS N
2.1.4 EQUIPMENT 29
2.1.5 CELLS USED 30
2.1.6 CELL CULTURE MEDIA 31
HTTP://D-NB.INFO/1052076521
2.1.7 ANTIBODIES
2.1.8 PEPTIDES
2.1.9 BUFFERS AND SOLUTIONS
2.2 METHODS.
2.2.1 CELL CULTURE AND HANDLING
2.2.2 CELL CULTURE FLASK COATING
2.2.3 CELL COUNTING
2.2.4 FREEZING AND THAWING OF MAMMALIAN CELLS
2.2.5 BONE MARROW ISOLATION AND BMDMS (BONE MARROW-DERIVED MACROPHAGES)
DIFFERENTIATION
2.2.6 FACS ANALYSIS OF MACROPHAGE DIFFERENTIATION
2.2.7 PEPTIDE FIBRIL FORMATION
2.2.8 THIOFLAVIN T (THT)-BINDING ASSAY
2.2.9 CAPASE-1 ASSAY WITH THE MICROGLIAL BV2 CELL LINE (WITH OR WITHOUT
LPS STIMULATION). _
2.2.10 CASPASE-1 ASSAY WITH IMMORTALIZED PERITONEAL MOUSE MACROPHAGES
FROM WILD-TYPE
AND NLRP3
+
MICE
2.2.11 CASPASE-1 ASSAY WITH BONE MARROW-DERIVED MACROPHAGES (BMDMS)
2.2.12 CATHEPSIN B ASSAY
2.2.13 IL-1|3 ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY)
2.2.14 CELL VIABILITY MEASUREMENT WITH TRYPAN BLUE STAINING
2.2.15 CONFOCAL MICROSCOPY
2.2.16 ISOLATION OF LYSOSOMES FROM MOUSE MICROGLIAL CELLS
2.2.17 PEPTIDE KINETIC ASSAY
2.2.18 PEPTIDE DEGRADATION ASSAYS
2.2.19 CLEAVAGE ASSAY WITH LYSOSOMAL EXTRACTS
2.2.20 CLEAVAGE ASSAY WITH CATHEPSIN B
2.2.21 BICINCHONINIC ACID (BCA) PROTEIN ASSAY
2.2.22 RECEPTOR BINDING ASSAY WITH
125
L-LABELLED RIAPP
2.2.23 IMMUNOBLOT ANALYSIS WITH CELL SUPERNATANTS
2.2.24 IMMUNOBLOT ANALYSIS OF AMYLIRI RECEPTOR COMPLEX (AMY3) 50
2.2.25 ENDOCYTOSIS INHIBITION ASSAY SO
2.2.26 STATISTICAL ANALYSIS SI
3 RESULTS 52
3.1 IAPP MIMIC REDUCES AP40-INDUCED NLRP3 INFLAMMASOME ACTIVITY 52
3.2 FIBRILLAR LAPP-INDUCED CASPASE-1 ACTIVITY CAN BE PREVENTED BY IAPP
MIMIC, IAPP-
GL, IN BOTH MICROGLIAL CELLS AND BMDMS 56
3.3 AMYLOID PEPTIDE-INDUCED CASPASE-1 ACTIVATION AND ITS INHIBITION BY
IAPP MIMIC,
IAPP-GI, IS INFLAMMASOME-DEPENDENT 60
3.4 IAPP MIMIC, IAPP-GI, INHIBITS AP-INDUCED CATHEPSIN B ACTIVATION AND
LYSOSOMAL
RUPTURE IN MICROGLIAL CELLS 61
3.5 A04O, IAPP-GI AND THEIR HETERO-COMPLEXES ARE TARGETED TO LYSOSOMAL
COMPARTMENTS 63
3.6 PREFIBRILLAR HUMAN IAPP AND THE NON-AMYLOIDOGENIC RAT IAPP
CONTRIBUTE TO THE
INHIBITION OF AMYLOID-P-INDUCED CASPASE-1 ACTIVATION AND ENHANCEMENT OF
LYSOSOMAL DEGRADATION
69
3.7 NOT ANY ARBITRARY PEPTIDE CONTRIBUTES TO THE LYSOSOMAL DEGRADATION
OF AF340 73
3.8 ACCELERATED UPTAKE OF AP40/IAPP-GI HETERO-COMPLEX BY MOUSE
MICROGLIAL BV2
CELLS 76
3.9 A RECEPTOR-MEDIATED, CLATHRIN- AND DYNAMIN-DEPENDENT MECHANISM IS
INVOLVED IN
AP40+IAPP-GI HETEROCOMPLEX UPTAKE 78
4 DISCUSSION 86
5 SUMMARY AND CONCLUSION
6 ABBREVIATIONS
94
96
7 REFERENCES
8 ACKNOWLEDGEMENTS
9 DECLARATION
10 CURRICULUM VITAE
|
any_adam_object | 1 |
author | Aftabizadeh, Maryam 1974- |
author_GND | (DE-588)1047330415 |
author_facet | Aftabizadeh, Maryam 1974- |
author_role | aut |
author_sort | Aftabizadeh, Maryam 1974- |
author_variant | m a ma |
building | Verbundindex |
bvnumber | BV042275767 |
ctrlnum | (OCoLC)884287051 (DE-599)DNB1052076521 |
dewey-full | 570 610 |
dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
dewey-ones | 570 - Biology 610 - Medicine and health |
dewey-raw | 570 610 |
dewey-search | 570 610 |
dewey-sort | 3570 |
dewey-tens | 570 - Biology 610 - Medicine and health |
discipline | Biologie Medizin |
format | Thesis Book |
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spelling | Aftabizadeh, Maryam 1974- Verfasser (DE-588)1047330415 aut Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity vorgelegt von Maryam Aftabizadeh 2014 119 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Aachen, Techn. Hochsch., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713241&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Aftabizadeh, Maryam 1974- Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity |
subject_GND | (DE-588)4113937-9 |
title | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity |
title_auth | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity |
title_exact_search | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity |
title_full | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity vorgelegt von Maryam Aftabizadeh |
title_fullStr | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity vorgelegt von Maryam Aftabizadeh |
title_full_unstemmed | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity vorgelegt von Maryam Aftabizadeh |
title_short | Effects of amyloid polypeptides and their inhibitors on NLRP3 inflammasome activity |
title_sort | effects of amyloid polypeptides and their inhibitors on nlrp3 inflammasome activity |
topic_facet | Hochschulschrift |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027713241&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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