Membrane trafficking:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2015
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
1270 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 457 S. Ill., graph. Darst. |
| ISBN: | 9781493923083 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804152836837081088 |
|---|---|
| adam_text | Titel: Membrane trafficking
Autor: Tang, Bor Luen
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xi
1 Intracellular Parcel Service: Current Issues in Intracellular
Membrane Trafficking........................................... 1
Johannes M. Herrmann and Anne Span,g
Part I Biochemical and Molecular Genetics Approaches
2 In Vitro Analysis of the Mitochondrial Preprotein Import Machinery
Using Recombinant Precursor Polypeptides.......................... 15
Dorothea Becker and Wolfyang Voos
3 Import of Proteins into Isolated Yeast Mitochondria.................... 37
Valentina Peleh, Ajay Ramesh, and Johannes M. Herrmann
4 Evaluation of Unconventional Protein Secretion
in Saccharomyces cerevisiae........................................ 51
Natsuko Miura and Mitsuyoshi Ueda
5 Fractionation of Plasmodium-Infected Human Red Blood Cells
to Study Protein Trafficking...................................... 71
Simone Külzer, Verena Bittl, and Jude M. Przyborski
6 Investigating Signaling Processes in Membrane Trafficking............... 81
Laura J. Sharpe and Andrew J. Brown
7 Recruitment of Coat Proteins to Liposomes and Peptidoliposomes......... 91
Sonja Huser, Gregor Suri, Pascal Crottet, and Martin Spiess
8 A ß-Lactamase Based Assay to Measure Surface Expression
of Membrane Proteins........................................... 107
Pieter Beerepoot, Vincent M. Lam, and Ali Salahpour
9 Cell-Free Reconstitution of Multivesicular Body (MVB) Cargo Sorting...... 115
Monica Gireud, Natalie Sirisaengtaksin, Susan Tsunoda,
and Andrew J. Bean
10 Analysis of Biogenesis of Lipid Droplets by Examining Rab40c
Associating with Lipid Droplets.................................... 125
Ran Tan, Xiaohui Xu, Wanjin Hong, and Tuanlao Wang
11 Analysis of Conventional and Unconventional Trafficking
of CFTR and Other Membrane Proteins............................. 137
Heon Tung Gee, Joo Toung Kim, and Min Goo Lee
12 Assessing Mammalian Autophagy.................................. 155
Sharon A. Tooze, Hannah C. Dooley, Harold BJ. Jefferies,
Justin Joachim, Delphine Judith, Christopher A Lamb, Minoo Razi,
and Martina Wirth
vii
viii
Contents
13 Expression of Functional Myc-Tagged Conserved Oligomeric
Golgi (COG) Subcomplexes in Mammalian Cells...................... 167
Rose A. Willett, Tetyana A. Kudlyk, and Vladimir V. Lupashin
14 Molecular and Cellular Characterization of GCC185:
A Tethering Protein of the Tra»f-Golgi Network...................... 179
Pak-yan Patricia Cheung and Suzanne R. Pfeffer
15 Visualizing Toll-Like Receptor-Dependent Phagosomal Dynamics
in Murine Dendritic Cells Using Live Cell Microscopy.................. 191
Adriana R Mantegazza and Michael S. Marks
16 Understanding of Complex Protein Interactions with Respect
to Anchorage Independence...................................... 205
Jill M. Tham, Sofie C. Van Huffel, and Wanjin Hong
17 Application of Flow Cytometry to Analyze Intracellular Location
and Trafficking of Cargo in Cell Populations.......................... 227
Wei Hong Toh, Fiona J. Houghton, Pei Zhi Cheryl Chia,
Yasmin M. Ramdzan, Danny M. Hatters, and Paul A. Gleeson
18 Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking
of Epidermal Growth Factor Receptor (EGFR)........................ 239
Christelle En Lin Chua, Angeline Tim Kiat Lim, and Bor Luen Tang
Part II Microscopy Methods and Imaging Approaches
19 Does Super-Resolution Fluorescence Microscopy Obsolete
Previous Microscopic Approaches to Protein Co-localization?............. 255
Laura MacDonald, Giulia Baldini, and Brian Storrie
20 Bimolecular Fluorescence Complementation (BiFC) Technique
in Yeast Saccharomyces cerevisiae and Mammalian Cells.................. 277
Marion Weber-Boyvat, Shiqian Li, Kari-Pekka Skarp, Vesa M. Olkkonen,
Daoguang Tan, and Jussi Jdntti
21 Microscopic and Spectroscopic Techniques to Investigate Lipid
Droplet Formation and Turnover in Yeast............................ 289
Heimo Wolinski and Sepp D. Kohlwein
22 Image-Based Identification of Nuclear Export Inhibitors
from Natural Products.......................................... 307
Bastien Cautain, Nuria de Pedro, Fernando Reyes, and Wolfgang Link
23 Correlative Video-Light-Electron Microscopy of Mobile Organelles........ 321
Galina V. Beznoussenko and Alexander A Mironov
24 Live Cell Imaging of Endosomal Trafficking in Fungi................... 347
Sebastian Baumann, Norio Takeshita, N. Grün, Reinhard Fischer,
and Michael Feldbrügge
25 Quantitative Analysis of Transferrin Cycling
by Automated Fluorescence Microscopy............................. 365
David T. Hirschmann, Christoph A Kasper, and Martin Spiess
Contents ix
Part III Omics Approaches
26 Identification of Factors Regulating MET Receptor Endocytosis
by High-Throughput siRNA Screening.............................. 381
Ivana Gaziova, Robert A. Davey, and Lisa A. Elferink
27 Large-Scale Analysis of Membrane Transport in Yeast
Using Invertase Reporters........................................ 395
Lauren Dalton, Michael Davey, and Elizabeth Conibear
28 RNAi Screens for Genes Involved in Golgi Glycosylation................. 411
Germaine T. Goh and Frederic A. Bard
29 Proteomic Analyses of a Bi-Lobed Structure in Trypanosoma brucei......... 427
Ladan Gheiratmand and Cynthia T. He
30 Application of the Proximity-Dependent Assay
and Fluorescence Imaging Approaches to Study Viral Entry Pathways....... 437
Alex Lipovsky, Wei Zhang, Akiko Iwasaki, and Daniel DiMaio
Index............................................................... 453
|
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| language | English |
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| spelling | Membrane trafficking ed. by Bor Luen Tang 2. ed. New York Humana Press 2015 XV, 457 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1270 Springer protocols Tang, Bor Luen edt Methods in molecular biology 1270 (DE-604)BV035362695 1270 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027712794&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Membrane trafficking Methods in molecular biology |
| title | Membrane trafficking |
| title_auth | Membrane trafficking |
| title_exact_search | Membrane trafficking |
| title_full | Membrane trafficking ed. by Bor Luen Tang |
| title_fullStr | Membrane trafficking ed. by Bor Luen Tang |
| title_full_unstemmed | Membrane trafficking ed. by Bor Luen Tang |
| title_short | Membrane trafficking |
| title_sort | membrane trafficking |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027712794&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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