Opioid receptors: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1230 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XV, 326 p. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Opioid receptors |b methods and protocols |c ed. by Santi M. Spampinato |
| 264 | 1 | |a New York |b Humana Press |c 2015 | |
| 300 | |a XV, 326 p. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 1230 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical references and index | ||
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| 650 | 2 | |a Récepteurs aux opioïdes | |
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Datensatz im Suchindex
| _version_ | 1804152753547640832 |
|---|---|
| adam_text | Titel: Opioid receptors
Autor: Spampinato, Santi M
Jahr: 2015
Contents
Dedication....................................................... v
Preface.......................................................... vii
Contributors.......................................................... xiii
Part I Genetic, Structural Biology, Transcriptional
AND POST-TRANSCRIPTIONAL ANALYSIS OF OPIOID RECEPTORS
1 Overview of Genetic Analysis of Human Opioid Receptors............... 3
Santi M. Spampinato
2 Computational Structural Biology of Opioid Receptors.................. 13
Davide Provasi
3 Analysis of Epigenetic Mechanisms Regulating Opioid Receptor
Gene Transcription............................................. 39
Cheol Kyu Hwang, Tadav Wagley, Ping-Tee Law, Li-Na Wei,
and Horace H. Loh
4 Renilla Luciferase Reporter Assay to Study 3 UTR-Driven
Posttranscriptional Regulations of OPRM1........................... 53
Gabriele Vincelli and Andrea Bedini
5 High-Throughput Gene Expression Profiling
of Opioid-Induced Alterations in Discrete Brain Areas................... 65
Michal Korostynski, Marcin Piechota, Slawomir Golda,
and Ryszard Przewlocki
Part II Cellular Detection and Analysis
of Opioid Receptors
6 Real-Time Imaging of Mu Opioid Receptors
by Total Internal Reflection Fluorescence Microscopy................... 79
Cristina Roman-Vendrell and Guillermo Ariel Tudowski
7 In Vivo Techniques .to Investigate the Internalization Profile
of Opioid Receptors............................................ 87
Amynah A. Pradhan, Vivianne L. Tawfik, Alycia P. Tipton,
and Gregory Scherrer
8 Monitoring Opioid Receptor Dimerization in Living Cells
by Bioluminescence Resonance Energy Transfer (BRET)................. 105
Monica Baiula
9 Bioluminescence Resonance Energy Transfer (BRET)
to Detect the Interactions Between Kappa Opioid Receptor
and Non-visual Arrestins......................................... 115
Andrea Bedini
ix
X
Contents
10 Identification and Verification of Proteins Interacting
with the Kappa Opioid Receptor (KOPR)............................ 129
Chongguang Chen, Peng Huang, and Lee-Yuan Liu-Chen
11 Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation
in Bat and Mouse Brains by Western Blot with Anti-pC,
an Affinity-Purified Polyclonal Anti-MOPR Antibody................... 141
Peng Huang, Chongguang Chen, and Lee-Yuan Liu-Chen
12 Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues .... 155
Yvonne Schmidt and Halina Machelska
Part III Analysis of Signaling Events Modulated
by Opioid Receptors
13 [35S]GTPyS Autoradiography for Studies
of Opioid Receptor Functionality.................................. 169
Alfljild Grönbladh and Mathias Hallberg
14 Fluorescence-Based, High-Throughput Assays
for p-Opioid Receptor Activation Using a Membrane
Potential-Sensitive Dye.......................................... 177
Alisa Knapman and Mark Connor
15 Analysis of Potassium and Calcium Imaging to Assay the Function
of Opioid Receptors............................................ 187
Viola Spahn, Dinah Nockemann, and Halina Machelska
16 Electrophysiological Patch Clamp Assay to Monitor the Action
of Opioid Receptors............................................ 197
Viola Spahn, Dinah Nockemann, and Halina Machelska
Part IV Model Systems to Studying Opioid
Receptor-Mediated Functions
17 Skin-Nerve Preparation to Assay the Function of Opioid Receptors
in Peripheral Endings of Sensory Neurons............................ 215
Rabih Moshourab, Yvonne Schmidt, and Halina Machelska
18 Mechanical Nociception Measurement in Mice and Rats
with Automated Von Frey Equipment............................... 229
Gabriele Campana and Roberto Rimondini
19 Detecting Zinc Release Induced by Mu-Opioid Receptor
Agonists in Brain Slices.......................................... 233
Maria Rodriguez-Munoz, Pilar Sdnchez-Bldzquez, Concha Bailôn,
and Javier Garzôn
20 Opioid Receptors: Methods for Detection and Their Modes
of Actions in the Eye............................................ 243
Shahid Husain
21 Evaluation of Murine Macrophage Cytokine Production
After In Vivo Morphine Treatment................................. 253
Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai,
and Paola Sacerdote
Contents xi
22 Measurement of Macrophage Toll-Like Receptor 4 Expression
After Morphine Treatment....................................... 263
Mara Castelli, Alberto Panerai, Paola Sacerdote, and Silvia Franchi
23 The Role of Opioid Receptors in Migration and Wound Recovery
In Vitro in Cultured Human Keratinocytes and Fibroblasts............... 273
Mei Bigliardi-Qi and Paul L. Bißliardi
Part V Behavioral Effects Mediated by Opioid Receptors
24 Role of Opioid Receptors in the Reinstatement
of Opioid-Seeking Behavior: An Overview........................... 281
Liana Fattore, Paola Fadda, Silvia Antinori, and Walter Fratta
25 Analysis of Opioid-Seeking Reinstatement in the Rat.................... 295
Liana Fattore, Paola Fadda, Mary Tresa Zanda, and Walter Fratta
26 Induction of a High Alcohol Consumption in Rats and Mice:
Role of Opioid Receptors........................................ 309
Roberto Rimondini and Gabriele Campana
27 Evaluation of Social and Nonsocial Behaviors Mediated
by Opioids in Mouse Pups....................................... 313
Francesca R. D Amato
Index............................................................... 323
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| indexdate | 2024-07-10T01:15:43Z |
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| language | English |
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| physical | XV, 326 p. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Opioid receptors methods and protocols ed. by Santi M. Spampinato New York Humana Press 2015 XV, 326 p. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1230 Springer protocols Includes bibliographical references and index Receptors, Opioid Récepteurs aux opioïdes Spampinato, Santi M. Sonstige (DE-588)1063952883 oth Methods in molecular biology 1230 (DE-604)BV035362695 1230 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027662287&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Opioid receptors methods and protocols Methods in molecular biology Receptors, Opioid Récepteurs aux opioïdes |
| title | Opioid receptors methods and protocols |
| title_auth | Opioid receptors methods and protocols |
| title_exact_search | Opioid receptors methods and protocols |
| title_full | Opioid receptors methods and protocols ed. by Santi M. Spampinato |
| title_fullStr | Opioid receptors methods and protocols ed. by Santi M. Spampinato |
| title_full_unstemmed | Opioid receptors methods and protocols ed. by Santi M. Spampinato |
| title_short | Opioid receptors |
| title_sort | opioid receptors methods and protocols |
| title_sub | methods and protocols |
| topic | Receptors, Opioid Récepteurs aux opioïdes |
| topic_facet | Receptors, Opioid Récepteurs aux opioïdes |
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