The role of endothelial cell laminins in mechanotransduction processes of small arteries:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 96 S. Ill., graph. Darst. 21 cm |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
ACKNOWLEDGMENTS I
LIST OF FIGURES V
LIST OF TABLES VII
ABSTRACT VLLL
ABBREVIATIONS X
INTRODUCTION 1
1 RESISTANCE ARTERIES: IMPORTANT REGULATORS OF SYSTEMIC BLOOD PRESSURE 1
2 CELLULAR AND EXTRACELLULAR COMPONENTS OF RESISTANCE ARTERIES 2
3 BASEMENT MEMBRANES 5
3.1 GENERAL FUNCTIONS OF LAMININS 8
3.2 LAMININ RECEPTORS 9
3.3 LAMININS IN ENDOTHELIAL CELL BASEMENT MEMBRANES: DISTRIBUTION AND
FUNCTIONS 11
4 ENDOTHELIAL MECHANOTRANSDUCTION 13
4.1 CYTOSKELETON AND NUCLEUS 14
4.2 LUMINAL MEMBRANE STRUCTURES 15
4.3 CELL JUNCTIONS AND ADHESION STRUCTURES 17
AIMS 19
MATERIALS AND METHODS 20
1 LABORATORY EQUIPMENT 20
2 CHEMICALS AND SOLUTIONS 21
3 MOUSE STRAINS 24
4 IMMUNOFLUORESCENCE STAINING 25
4.1 CRYOSECTIONS 25
4.1 WHOLE MOUNT IMMUNOFLUORESCENCE STAINING OF ISOLATED VESSELS 25
5 PRESSURE MYOGRAPH ANALYSES 29
5.1 FLOW-INDUCED DILATION 29
5.2 MYOGENIC RESPONSE 30
5.3 PASSIVE PRESSURE-DIAMETER RELATIONSHIP 31
6
WIRE MYOGRAPH ANALYSES 31
111
HTTP://D-NB.INFO/1059820676
CONTENTS
7 ATOMIC FORCE MICROSCOPY 32
7.1 ENDOTHELIAL CELL CULTURES 32
7.2 EX-VIVO ARTERIES 33
7.3 AFM FORCE-DISTANCE CURVE ANALYSIS 34
8 ELECTRON MICROSCOPY 34
8.1 TRANSMISSION ELECTRON MICROSCOPY 34
8.2 SCANNING ELECTRON MICROSCOPY OF ENDOTHELIUM-DENUDED ARTERIES 35
9 CELL CULTURE 36
9.1 CELL LINES 36
9.2 GROWTH CONDITION AND GROWTH MEASUREMENT 36
10 STATISTIC 37
11 IMAGE ANALYSES 37
RESULTS 38
1 MORPHOLOGICAL CHARACTERIZATION OF THE ENDOTHELIAL CELL SPECIFIC
LAMININ A5
KNOCK OUT MOUSE 38
2 FUNCTIONAL ANALYSES OFTIE2CRELAMA5-/- AND LAMA4-/- RESISTANCE ARTERIES
44
2.1 FLOW-INDUCED DILATION OF MESENTERIC ARTERIES 44
2.2 RESPONSE TO VASODILATORY OR VASOCONSTRICTORY DRUGS 46
2.3 MYOGENIC RESPONSE: SMOOTH MUSCLE RESPONSE TO VESSEL WALL STRETCH 47
3 HOW DOES LAMININ A5 IMPACT ON MECHANOTRANSDUCTION? 50
3.1 FOCAL ADHESIONS 50
3.2 INTEGRIN A5(31, A LAMININ A5 RECEPTOR, AND MAIN COMPONENT OF FOCAL
ADHESIONS IN ENDOTHELIAL CELLS OF MESENTERIC ARTERIES 50
4 LAMININ A5 IMPACTS ON THE TENSIONAL INTEGRITY OF ENDOTHELIAL CELLS 55
5 VE-CADHERIN PHOSPHORYLATION IN ENDOTHELIUM OF LAMA4MESENTERIC ARTERIES
58
6 ADDITIONAL OBSERVATIONS: THE LACK OF LAMININ A5 COULD IMPACT ON TISSUE
MICRO
ELASTICITY LEADING TO DIFFERENTIAL NUCLEAR STIFFNESS 61
DISCUSSION 63
1 FOCAL ADHESIONS MORPHOLOGY AND INTEGRIN COMPOSITION 65
2 BM IMPACT ON REGULATION OF ADHERENCE JUNCTION 67
3 FUTURE PROSPECTS 70
4 CONCLUSION 72
REFERENCE 74
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| language | English |
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| spelling | Di Russo, Jacopo 1984- Verfasser (DE-588)105982065X aut The role of endothelial cell laminins in mechanotransduction processes of small arteries vorgelegt von Jacopo Di Russo 2014 96 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Münster (Westfalen), Univ., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027626077&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Di Russo, Jacopo 1984- The role of endothelial cell laminins in mechanotransduction processes of small arteries |
| subject_GND | (DE-588)4113937-9 |
| title | The role of endothelial cell laminins in mechanotransduction processes of small arteries |
| title_auth | The role of endothelial cell laminins in mechanotransduction processes of small arteries |
| title_exact_search | The role of endothelial cell laminins in mechanotransduction processes of small arteries |
| title_full | The role of endothelial cell laminins in mechanotransduction processes of small arteries vorgelegt von Jacopo Di Russo |
| title_fullStr | The role of endothelial cell laminins in mechanotransduction processes of small arteries vorgelegt von Jacopo Di Russo |
| title_full_unstemmed | The role of endothelial cell laminins in mechanotransduction processes of small arteries vorgelegt von Jacopo Di Russo |
| title_short | The role of endothelial cell laminins in mechanotransduction processes of small arteries |
| title_sort | the role of endothelial cell laminins in mechanotransduction processes of small arteries |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027626077&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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