Cytotoxic T-cells: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press [u.a.]
2014
|
| Schriftenreihe: | Methods in molecular biology
1186 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Ranieri, Elena: Cytotoxic T-Cells |
| Beschreibung: | XII, 301 S. Ill., graph. Darst. |
| ISBN: | 9781493911578 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804152553210904577 |
|---|---|
| adam_text | Titel: Cytotoxic T-cells
Autor: Ranieri, Elena
Jahr: 2014
Contents
Preface.......................................................... v
Acknowledgement.................................................. vii
Contributors.......................................................... xi
1 Negative and Positive Separation Techniques for the Isolation
of Antigen-Specific CD8+ T Cells from Blood and Tumor Tissue........... 1
Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri
2 In Vitro Ex Vivo Generation of Cytotoxic T Lymphocytes............... 13
Margherita Gigante and Elena Ranieri
3 Phenotypic and Functional Characterization of Cytotoxic
T Lymphocytes by Flow Cytometry................................ 21
Iulia Popescu, Matthew Pipeling, Jason Akulian, and John McDyer
4 Intracellular Signaling of CTLs.................................... 49
Sharon Natasha Cox
5 Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR....... 65
Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus
6 CTLELISPOT Assay........................................... 75
Elena Ranieri, Iulia Popescu, and Margherita Gigante
7 Methods of Purification of CTL-Derived Exosomes.................... 87
Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
8 Characterization of CTL by Microscopy............................. 103
Giuseppe Stefano Netti
9 Intravital Imaging of Cytotoxic T Lymphocytes....................... 121
Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi
10 The Microarray-Based Approach for the Analysis of the Transcriptome...... 131
Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo
11 miRNome Analysis Using Real-Time PCR........................... 201
Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo
12 Proteomic Approaches by SELDI and MALDI-TOF/MS
for CTL Analysis............................................... 233
Massimo Papale and Maria Teresa Rocchetti
13 Two-Dimensional Gel Electrophoresis Approach for CTL
Phosphoproteome Analysis....................................... 243
Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo
14 Targeting the JAK/STAT Pathway in Cytotoxic
T lymphocytes (CTL) by Next Generation Sequencing (NGS)............ 253
Maddalena Gigante, Sterpeta Diella, and Elena Ranieri
ix
X
Contents
15 Experimental Model for Studying the Involvement of Regulatory
Cytotoxic T Cells in Bone Resorption............................... 269
Giacomina Brunetti, Angela Oranger, Silvia Colucci,
and Maria Grano
16 CTL and Transplantation: Tissue In Vivo Characterization............... 283
Federica Rascio, Chiara Divella, and Giuseppe Grandaliano
Index............................................................... 295
|
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| discipline | Biologie |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T01:12:32Z |
| institution | BVB |
| isbn | 9781493911578 |
| language | English |
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| physical | XII, 301 S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Humana Press [u.a.] |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Cytotoxic T-cells methods and protocols ed. by Elena Ranieri New York Humana Press [u.a.] 2014 XII, 301 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1186 Springer protocols Ranieri, Elena: Cytotoxic T-Cells Killerzelle (DE-588)4163776-8 gnd rswk-swf Killerzelle (DE-588)4163776-8 s DE-604 Ranieri, Elena (DE-588)106086472X edt Methods in molecular biology 1186 (DE-604)BV035362695 1186 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027534749&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytotoxic T-cells methods and protocols Methods in molecular biology Killerzelle (DE-588)4163776-8 gnd |
| subject_GND | (DE-588)4163776-8 |
| title | Cytotoxic T-cells methods and protocols |
| title_auth | Cytotoxic T-cells methods and protocols |
| title_exact_search | Cytotoxic T-cells methods and protocols |
| title_full | Cytotoxic T-cells methods and protocols ed. by Elena Ranieri |
| title_fullStr | Cytotoxic T-cells methods and protocols ed. by Elena Ranieri |
| title_full_unstemmed | Cytotoxic T-cells methods and protocols ed. by Elena Ranieri |
| title_short | Cytotoxic T-cells |
| title_sort | cytotoxic t cells methods and protocols |
| title_sub | methods and protocols |
| topic | Killerzelle (DE-588)4163776-8 gnd |
| topic_facet | Killerzelle |
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