RNA mapping: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1182 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references |
| Beschreibung: | XI, 375 S. Ill., graph. Darst. 26 cm |
| ISBN: | 9781493910618 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804152496629743616 |
|---|---|
| adam_text | Titel: RNA mapping
Autor: Alvarez, M. Lucrecia
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... ix
Part I Structural RNA Mapping
1 Full-Length Characterization of Transcribed Genomic Regions............ 3
Marc R. Reboll, M. Lucrecia Alvarez, and Mahtab Nourbakhsh
2 Rapid Mapping of RNA 3 and 5 Ends.............................. 19
Victoria Zismann and Mahtab Nourbakhsh
3 Single Nucleotide Mapping of RNA 5 and 3 Ends..................... 27
Mahtab Nourbakhsh
4 Analysis of RNA Secondary Structure............................... 35
Mahtab Nourbakhsh
5 Tertiary Structure Mapping of the Pri-miRNA miR-17-92............... 43
Steven G. Chaulk and Richard P. Fahlman
6 In Situ Hybridization Detection of miRNA Using
LNA™ Oligonucleotides......................................... 57
Stefania Cotta Doné and Olga Beltcheva
7 Quantification of miRNAs by a Simple and Specific qPCR Method......... 73
Susanna Cirera and Peter K. Busk
8 RNA Isolation for Small RNA Next-Generation Sequencing
from Acellular Biofluids.......................................... 83
Kasandra L. Burgos and Kendall Van Keuren-Jensen
9 Sequencing Small RNA: Introduction and Data Analysis Fundamentals...... 93
Jai Prakash Mehta
10 Measuring Expression Levels of Small Regulatory RNA Molecules
from Body Fluids and Formalin-Fixed, Paraffin-Embedded Samples........ 105
Adrienn Gyongyosi, Otto Docs, Zsolt Czimmerer,
Laszlo Orosz, Attila Horvath, Olga Török, Gabor Mehes,
Laszlo Nagy, and BalintL. Balint
11 MicroRNA Profiling in Plasma or Serum Using Quantitative RT-PCR...... 121
Marina C. Costa, Ana Lucia Leitäo, and Francisco J. Enguita
12 MicroRNA Profiling of Exosomes Isolated from Biofluids
and Conditioned Media......................................... 131
Sweta Rani
13 Isolation of Urinary Exosomes for RNA Biomarker Discovery
Using a Simple, Fast, and Highly Scalable Method..................... 145
M. Lucrecia Alvarez
vii
viii Contents
Part II Functional RNA Mapping
14 Identification of Actively Translated mRNAs.......................... 173
Marc R. Reboll and Mahtab Nourbakhsh
15 Mapping of Internal Ribosome Entry Sites (1RES)..................... 179
Sarah Mehrtens and Marc R. Reboll
16 Mapping of Protein Binding RNA Elements.......................... 187
Marc R. Reboll
17 Purification of RNA-Binding Proteins............................... 195
Birgit Ritter and Marc R. Reboll
18 De Novo Approach to Classify Protein-Coding and Noncoding
Transcripts Based on Sequence Composition.......................... 203
Haitao Luo, Dechao Bu, Liang Sun, Runsheng Chen,
and Ti Zhao
19 Computational Methods to Predict Long Noncoding RNA Functions
Based on Co-expression Network.................................. 209
Ti Zhao, Haitao Luo, Xiaowei Chen, Ti Xiao, and Runsheng Chen
20 MicroRNA Biogenesis: Dicing Assay................................ 219
Carlos A. Melo and Sonia A. Melo
21 Faster Experimental Validation of microRNA Targets Using Cold
Fusion Cloning and a Dual Firefly-Renilla Luciferase Reporter Assay....... 227
M. Lucrecia Alvarez
22 Experimental Validation of Predicted Mammalian MicroRNAs
of Mirtron Origin.............................................. 245
Anita Schamberger and Tamds I. Orbdn
23 A Guide for miRNA Target Prediction and Analysis
Using Web-Based Applications.................................... 265
Ana Lucia Leitäo, Marina C. Costa, and Francisco J. Enguita
24 Tapping MicroRNA Regulation Networks Through Integrated
Analysis of MicroRNA-mRNA High-Throughput Profiles............... 279
Anthony D. Saleh and Hui Cheng
25 miRWalk Database for miRNA-Target Interactions..................... 289
Harsh Dweep, Norbert Greiz, and Carsten Sticht
26 A Schematic Workflow for Collecting Information About
the Interaction Between Copy Number Variants and MicroRNAs
Using Existing Resources........................................ 307
Harsh Dweep, Norbert Gretz, and Kyriakos Felekkis
27 SYBR® Green and TaqMan® Quantitative PCR Arrays: Expression
Profile of Genes Relevant to a Pathway or a Disease State................ 321
M. Lucrecia Alvarez and Stefania Cotta Doné
28 Comprehensive Meta-analysis of MicroRNA Expression
Using a Robust Rank Aggregation Approach.......................... 361
Urmo Vosa, Raivo Kolde, Jaak Vilo, Andres Metspalu,
and Tarmo Annilo
Index............................................................... 375
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| isbn | 9781493910618 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | RNA mapping methods and protocols ed. by M. Lucrecia Alvarez ; Mahtab Nourbakhsh New York, NY [u.a.] Humana Press 2014 XI, 375 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1182 Springer protocols Includes bibliographical references Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Alvarez, M. Lucrecia Sonstige (DE-588)1058984411 oth Nourbakhsh, Mahtab 1962- Sonstige (DE-588)113542984 oth Methods in molecular biology 1182 (DE-604)BV035362695 1182 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027499975&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | RNA mapping methods and protocols |
| title_auth | RNA mapping methods and protocols |
| title_exact_search | RNA mapping methods and protocols |
| title_full | RNA mapping methods and protocols ed. by M. Lucrecia Alvarez ; Mahtab Nourbakhsh |
| title_fullStr | RNA mapping methods and protocols ed. by M. Lucrecia Alvarez ; Mahtab Nourbakhsh |
| title_full_unstemmed | RNA mapping methods and protocols ed. by M. Lucrecia Alvarez ; Mahtab Nourbakhsh |
| title_short | RNA mapping |
| title_sort | rna mapping methods and protocols |
| title_sub | methods and protocols |
| topic | Molekularbiologische Methode (DE-588)7650063-9 gnd RNS (DE-588)4076759-0 gnd |
| topic_facet | Molekularbiologische Methode RNS Aufsatzsammlung |
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