Stable isotope labeling by amino acids in cell culture (SILAC): methods and protocols
Gespeichert in:
Weitere Verfasser: | |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
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Schriftenreihe: | Methods in molecular biology
1188 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | Includes bibliographical references |
Beschreibung: | XIV, 372 S. Ill., graph. Darst. 26 cm |
ISBN: | 9781493911417 |
Internformat
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Datensatz im Suchindex
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adam_text | Titel: Stable isotope labeling by amino acids in cell culture (SILAC)
Autor: Warscheid, Bettina
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... xi
1 Fifteen Years of Stable Isotope Labeling by Amino Acids
in Cell Culture (SILAC)......................................... 1
Matthias Mann
2 Stable Isotope Labeling by Amino Acids Applied
to Bacterial Cell Culture......................................... 9
Boumediene Soufi and Boris Macek
3 SILAC Labeling of Yeast for the Study of Membrane Protein Complexes .... 23
Silke Oeljeklaus, Andreas Schummer, Ida Suppanz,
and Bettina Warscheid
4 Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei
Using Stable Isotope Labeling by Amino Acids in Cell Culture
and Mass Spectrometry.......................................... 47
Olivera Cirovic and Torsten Ochsenreiter
5 Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons...... 57
Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert
6 SILAC and Alternatives in Studying Cellular Proteomes of Plants.......... 65
Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze
7 In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster . . 85
Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach
8 Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse........ 95
Soraya Hölper, Aaron Ruhs, and Marcus Krüger
9 Identification of Novel Protein Functions and Signaling
Mechanisms by Genetics and Quantitative Phosphoproteomics
in Caenorhabditis elegans......................................... 107
Julius Fredens, Kasper Engholm-Keller, Jakob Moller-Jensen,
Martin Rossel Larsen, and Nils J. Fargeman
10 SILAC-Based Temporal Phosphoproteomics.......................... 125
Chiara Francavilla, Omid Hekmat, Blagoy Blagoev,
and Jesper V. Olsen
11 Global Ubiquitination Analysis by SILAC in Mammahan Cells............ 149
Zhiping Wu, Chan Hyun Na, Haiyan Tan, and Junmin Peng
12 Quantifying In Vivo, Site-Specific Changes in Protein
Methylation with SILAC......................................... 161
Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong
¡X
X
Contents
13 Applying SILAC for the Differential Analysis of Protein Complexes......... 177
Karsten Boldt, Christian J. Gloeckner, Yves Texier,
Felix von Zweydorf and Marius Uejfing
14 Defining Dynamic Protein Interactions Using SILAC-Based
Quantitative Mass Spectrometry................................... 191
Xiaorong Wang and Lan Huang
15 Identifying Nuclear Protein-Protein Interactions Using GFP
Affinity Purification and SILAC-Based Quantitative Mass Spectrometry..... 207
H. Irem Baymaz, Cornelia G. Spruip, and Michiel Vermeulen
16 Analyzing the Protein Assembly and Dynamics of the Human
Spliceosome with SILAC......................................... 227
Carla Schmidt, Monika Raabe, Reinhard Ltthrmann,
and Henning Urlaub
17 Identification and Validation of Protein-Protein Interactions
by Combining Co-immunoprecipitation, Antigen Competition,
and Stable Isotope Labeling...................................... 245
Frederik Sommer, Timo Mühlhaus, Dorothea Hemme,
Daniel Veyel, and Michael Schroda
18 Protein Correlation Profiling-SILAC to Study
Protein-Protein Interactions...................................... 263
Anders R. Kristensen and Leonard J. Foster
19 Autophagosomal Proteome Analysis by Protein Correlation
Profiling-SILAC............................................... 271
Andrea C. Becker and Jörn Dengjel
20 Design and Application of Super-SILAC for Proteome Quantification....... 281
Yair Pozniak and Tamar Geiger
21 Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster
Larvae and Cells for In Vivo Functional Studies....................... 293
Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari,
and Tiziana Bonaldi
22 Analysis of Secreted Proteins Using SILAC........................... 313
Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova
23 Identification of MicroRNA Targets by Pulsed SILAC................... 327
Markus Kaller, Silke Oeljeklaus, Bettina Warscheid,
and Heiko Hermeking
24 MaxQuant for In-Depth Analysis of Large SILAC Datasets............... 351
Stefka Tyanova, Matthias Mann, and Jürgen Cox
Index............................................................... 365
|
any_adam_object | 1 |
author2 | Warscheid, Bettina 1971- |
author2_role | edt |
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bvnumber | BV042058361 |
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ctrlnum | (OCoLC)892502417 (DE-599)HBZHT018374066 |
dewey-full | 571.6/38 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 571 - Physiology & related subjects |
dewey-raw | 571.6/38 |
dewey-search | 571.6/38 |
dewey-sort | 3571.6 238 |
dewey-tens | 570 - Biology |
discipline | Biologie |
format | Book |
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id | DE-604.BV042058361 |
illustrated | Illustrated |
indexdate | 2024-07-10T01:11:37Z |
institution | BVB |
isbn | 9781493911417 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027499293 |
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owner | DE-11 DE-12 DE-355 DE-BY-UBR |
owner_facet | DE-11 DE-12 DE-355 DE-BY-UBR |
physical | XIV, 372 S. Ill., graph. Darst. 26 cm |
publishDate | 2014 |
publishDateSearch | 2014 |
publishDateSort | 2014 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols ed. by Bettina Warscheid New York, NY [u.a.] Humana Press 2014 XIV, 372 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1188 Springer protocols Includes bibliographical references Proteomics Tandem mass spectrometry Isotopenmarkierung (DE-588)4176811-5 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 s Isotopenmarkierung (DE-588)4176811-5 s Zellkultur (DE-588)4067547-6 s b DE-604 Warscheid, Bettina 1971- (DE-588)1031390898 edt Methods in molecular biology 1188 (DE-604)BV035362695 1188 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027499293&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols Methods in molecular biology Proteomics Tandem mass spectrometry Isotopenmarkierung (DE-588)4176811-5 gnd Zellkultur (DE-588)4067547-6 gnd Proteomanalyse (DE-588)4596545-6 gnd |
subject_GND | (DE-588)4176811-5 (DE-588)4067547-6 (DE-588)4596545-6 |
title | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols |
title_auth | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols |
title_exact_search | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols |
title_full | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols ed. by Bettina Warscheid |
title_fullStr | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols ed. by Bettina Warscheid |
title_full_unstemmed | Stable isotope labeling by amino acids in cell culture (SILAC) methods and protocols ed. by Bettina Warscheid |
title_short | Stable isotope labeling by amino acids in cell culture (SILAC) |
title_sort | stable isotope labeling by amino acids in cell culture silac methods and protocols |
title_sub | methods and protocols |
topic | Proteomics Tandem mass spectrometry Isotopenmarkierung (DE-588)4176811-5 gnd Zellkultur (DE-588)4067547-6 gnd Proteomanalyse (DE-588)4596545-6 gnd |
topic_facet | Proteomics Tandem mass spectrometry Isotopenmarkierung Zellkultur Proteomanalyse |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027499293&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT warscheidbettina stableisotopelabelingbyaminoacidsincellculturesilacmethodsandprotocols |