Protein affinity tags: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1177 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 213 S. Ill., graph. Darst. |
| ISBN: | 9781493910335 1493910337 |
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Datensatz im Suchindex
| _version_ | 1804152459517493248 |
|---|---|
| adam_text | Titel: Protein affinity tags
Autor: Giannone, Richard
Jahr: 2014
Contents
Preface........................................................... v
Contributors........................................................... ix
1 1D4: A Versatile Epitope Tag for the Purification and Characterization
of Expressed Membrane and Soluble Proteins.......................... 1
Laurie L. Molday and Robert S. Molday
2 Affinity Purification of Heme-Tagged Proteins......................... 17
Wesley B. Asher and Kara L. Bren
3 Purification of a Recombinant Protein with Cellulose-Binding
Module 3 as the Affinity Tag....................................... 35
Dongmei Wang and Jiong Hong
4 Purification of E. coli Proteins Using a Self-Cleaving
Chidn-Binding Affinity Tag....................................... 47
Michael J. Coolbaugh and David W. Wood
5 Simplified Protein Purification Using an Autoprocessing,
Inducible Enzyme Tag........................................... 59
Aimee Shen
6 SUMO as a Solubility Tag and In Vivo Cleavage
of SUMO Fusion Proteins with Ulpl................................ 71
Dennis Kuo, Minghua Nie, and Albert J. Courey
7 Rescuing Aggregation-Prone Proteins in Escherichia coli
with a Dual His6-MBP Tag........................................ 81
Danielle Needle and David S. Waugh
8 Expression, Purification, and Immobilization of Recombinant
Tamavidin 2 Fusion Proteins....................................... 95
Toshimitsu Takakura, Naomi Oka, and Masako Tsunashima
9 Use of Tandem Affinity Chromatography for Purification
of Cannabinoid Receptor CB2...................................... 107
Silvia C. Locatelli-Hoops and Alexei A. Teliseev
10 Detection of Protein-Protein Interactions Using Tandem
Affinity Purification.............................................. 121
Ian Goodfellow and Dalan Bailey
11 An Improved In Vivo Biotinylation Strategy Combined
with FLAG and Antibody Based Approaches for Affinity Purification
of Protein Complexes in Mouse Embryonic Stem Cells................... 135
Francesco Faiola, Arven Saunders, Baoyen Dang,
and Jianlong Wang
12 Purification of Recombinant Proteins with a Multifunctional GFP Tag....... 151
Takashi Murayama and Takuya Kobayashi
vii
viii Contents
13 Targeted Purification of SnAvi-Tagged Proteins........................ 163
Ursula Schaffer, Ralf Baumeister, and Ekkehard Schulze
14 An Efficient Fluorescent Protein-Based Multifunctional
Affinity Purification Approach in Mammalian Cells...................... 175
Hanhui Ma, Janel R. McLean, Kathleen L. Gould,
and Dannel McCollum
15 Bimolecular Affinity Purification: A Variation of TAP
with Multiple Applications........................................ 193
Retro Starokadomskyy and Ezra Burstein
Index................................................................ 211
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| isbn | 9781493910335 1493910337 |
| language | English |
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| spelling | Protein affinity tags methods and protocols ed. by Richard J. Giannone ... New York, NY [u.a.] Humana Press 2014 X, 213 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1177 Springer protocols Giannone, Richard edt Methods in molecular biology 1177 (DE-604)BV035362695 1177 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027477180&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein affinity tags methods and protocols Methods in molecular biology |
| title | Protein affinity tags methods and protocols |
| title_auth | Protein affinity tags methods and protocols |
| title_exact_search | Protein affinity tags methods and protocols |
| title_full | Protein affinity tags methods and protocols ed. by Richard J. Giannone ... |
| title_fullStr | Protein affinity tags methods and protocols ed. by Richard J. Giannone ... |
| title_full_unstemmed | Protein affinity tags methods and protocols ed. by Richard J. Giannone ... |
| title_short | Protein affinity tags |
| title_sort | protein affinity tags methods and protocols |
| title_sub | methods and protocols |
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