Verfügbarkeit: Effect of IL-15 on T-cell differentiation

Effect of IL-15 on T-cell differentiation:

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Bibliographische Detailangaben
1. Verfasser:	Koch, Flávia (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	2014
Schlagworte:	T-Lymphozyt Apoptosis Interleukin 15 Immunmodulation Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	165 S. Ill., graph. Darst.

Internformat

MARC


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adam_text	EFFECT OF IL-15 ON T-CELL DIFFERENTIATION DISSERTATION DER MATHEMATISCH-NATURWISSENSCHAFTLICHEN FAKULTAT DER EBERHARD KARLS UNIVERSITAT TUBINGEN ZUR ERLANGUNG DES GRADES EINES DOKTORS DER NATURWISSENSCHAFTEN (DR. RER. NAT.) VORGELEGT VON FLDVIA KOCH (GEB. RIBEIRO DA SILVA) AUS PORTO ALEGRE, BRASILIEN TUBINGEN 2014 HTTP://D-NB.INFO/1053384521 1 ZUSAMMENFASSUNG 8 2 SUMMARY 10 3 LIST OF ABBREVIATIONS 12 4 INTRODUCTION 15 4.1 IMMUNE SYSTEM: GENERAL ASPECTS 15 4.2 T-CELLS 16 4.2.1 V(D)J RECOMBINATION 16 4.2.2 THYMIC SELECTION 18 4.2.3 NAIVE T-CELL 19 4.2.4 T-CELL LINEAGES 19 4.2.5 ANTIGEN PRESENTATION 25 4.2.6 T-CELL ACTIVATION 27 4.2.7 T-CELL MEMORY 29 4.3 THE CRUCIAL ROLE OF YC CYTOKINES IN T-CELL BIOLOGY 34 4.4 LNTERLEUKIN-15 36 4.4.1 IL-15 RECEPTOR 39 4.4.2 T RANS-PRESENTATION 40 4.5 CYTOMEGALOVIRUS 41 4.5.1 VIRUS PROTEINS 45 4.6 IMMUNOSENESCENCE 47 4.7 TELOMERE SHORTENING AND REPLICATIVE SENESCENCE 49 5 AIM OF THE STUDY 52 6 MATERIALS 53 4 6.1 SUBJECTS 53 6.2 CELL CULTURE REAGENTS 55 6.2.1 MEDIA 55 6.2.2 CYTOKINES 55 6.2.3 PEPMIXES 56 6.3 CLONING 56 6.3.1 CLONES 56 6.3.2 TELOMERE LENGTH DETERMINATION 56 6.4 ELISA 57 6.5 IMMUNE FLUORESCENCE 58 6.5.1 ANTIBODIES 58 6.5.2 INTRACELLULAR STAINING 60 6.5.3 BUFFERS AND REAGENTS 60 6.6 OTHERS REAGENTS AND SOLUTIONS 61 6.7 EQUIPMENT 63 6.8 PLASTICWARE AND GENERAL MATERIALS 64 6.9 SOFTWARE 65 7 METHODS 66 7.1 CELL CULTURES 66 7.1.1 QUANTIFICATION OF CELL NUMBER AND VIABILITY 66 7.1.2 CRYOPRESERVATION OF CELLS 66 7.1.3 THAWING OF CRYOPRESERVED CELLS 67 7.1.4 CFSE LABELING 67 7.1.5 IL-15 TREATMENT 68 5 7.1.6 CMV LYSATE 68 7.1.7 PEPMIX 69 7.2 CLONES 69 7.2.1 PRIMING 69 7.2.2 CLONING 69 7.2.3 IRRADIATION OF CELLS 70 7.3 TELOMERE LENGTH 70 7.4 EUSA 72 7.5 FLOW CYTOMETRY AND IMMUNOFLUORESCENCE STAINING 73 7.5.1 OVERVIEW 73 7.5.2 LIGHT SCATTER 74 7.5.3 FLUORESCENCE AND COMPENSATION 75 7.5.4 STAINING 76 7.6 STATISTICAL ANALYSIS 79 8 RESULTS 80 8.1 IL-15 RECEPTOR 80 8.2 PROTECTIVE EFFECT OF IL-15 IN FAS-INDUCED APOPTOSIS 81 8.3 IFN-Y PRODUCTION 82 8.4 EFFECT OF IL-15 ON PBMCS IN CULTURE 85 8.5 IL-15 AND HCMV ANTIGENS (PP65 AND IE1) 86 8.5.1 MIDDLE-AGED DONORS: PHENOTYPE DISTRIBUTION 88 8.5.2 ELDERLY INDIVIDUALS: PHENOTYPE DISTRIBUTION 96 8.5.3 MIDDLE-AGED (55 YRS) INDIVIDUALS VERSUS ELDERLY (92 YRS) INDIVIDUALS FROM THE NETHERLANDS 99 8.5.4 PREVALENT PHENOTYPE IN EACH CELL DIVISION: 103 6 8.5.5 PROLIFERATIVE CAPACITY OF EACH T-CELL DIFFERENTIATION STAGE: MIDDLE- AGED DONORS..109 8.5.6 THE PROLIFERATIVE CAPACITY OF T-CELLS AT EACH DIFFERENTIATION STAGE: ELDERLY INDIVIDUALS (92 YRS) 112 8.5.7 GERMAN MIDDLE-AGED (55 YRS) DONORS VERSUS ELDERLY (92 YRS) INDIVIDUALS 114 8.6 ANTIGEN-SPECIFIC CELLS IN ELDERLY INDIVIDUALS FROM CANADA 118 8.7 T-CELL CLONES 122 8.7.1 PEPMIXES AND CLONES 122 8.7.2 PROLIFERATION 123 8.7.3 TELOMERE LENGTH 125 8.7.4 LONG-TERM EXPANSION OF THE CLONES 126 8.7.1 EXPANSION OF T-CELL CLONES WITH PEPMIX STIMULATION 129 9 DISCUSSION 130 9.1 EFFECT OF IL-15 ON PBMCS IN CULTURE 131 9.1.1 IL-15 RECEPTOR 131 9.1.2 IL-15 AND APOPTOSIS 133 9.1.3 IFN-Y PRODUCTION 134 9.1.4 IL-15 AND THE PROLIFERATIVE CAPACITY OF T-CELLS 134 9.1.5 ANTIGEN-SPECIFIC CELLS IN ELDERLY INDIVIDUALS FROM CANADA 140 9.2 CLONES 141 10 CONCLUSIONS AND FUTURE DIRECTIONS 144 11 ACKNOWLEDGMENTS 146 12 REFERENCE LIST 147 7
any_adam_object	1
author	Koch, Flávia
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spelling	Koch, Flávia Verfasser (DE-588)1052100368 aut Effect of IL-15 on T-cell differentiation vorgelegt von Flávia Koch (geb. Ribeiro da Silva) 2014 165 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2014 T-Lymphozyt (DE-588)4127387-4 gnd rswk-swf Apoptosis (DE-588)4291096-1 gnd rswk-swf Interleukin 15 (DE-588)4606774-7 gnd rswk-swf Immunmodulation (DE-588)4315157-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Interleukin 15 (DE-588)4606774-7 s T-Lymphozyt (DE-588)4127387-4 s Immunmodulation (DE-588)4315157-7 s Apoptosis (DE-588)4291096-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027476411&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Koch, Flávia Effect of IL-15 on T-cell differentiation T-Lymphozyt (DE-588)4127387-4 gnd Apoptosis (DE-588)4291096-1 gnd Interleukin 15 (DE-588)4606774-7 gnd Immunmodulation (DE-588)4315157-7 gnd
subject_GND	(DE-588)4127387-4 (DE-588)4291096-1 (DE-588)4606774-7 (DE-588)4315157-7 (DE-588)4113937-9
title	Effect of IL-15 on T-cell differentiation
title_auth	Effect of IL-15 on T-cell differentiation
title_exact_search	Effect of IL-15 on T-cell differentiation
title_full	Effect of IL-15 on T-cell differentiation vorgelegt von Flávia Koch (geb. Ribeiro da Silva)
title_fullStr	Effect of IL-15 on T-cell differentiation vorgelegt von Flávia Koch (geb. Ribeiro da Silva)
title_full_unstemmed	Effect of IL-15 on T-cell differentiation vorgelegt von Flávia Koch (geb. Ribeiro da Silva)
title_short	Effect of IL-15 on T-cell differentiation
title_sort	effect of il 15 on t cell differentiation
topic	T-Lymphozyt (DE-588)4127387-4 gnd Apoptosis (DE-588)4291096-1 gnd Interleukin 15 (DE-588)4606774-7 gnd Immunmodulation (DE-588)4315157-7 gnd
topic_facet	T-Lymphozyt Apoptosis Interleukin 15 Immunmodulation Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027476411&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT kochflavia effectofil15ontcelldifferentiation

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