Innate DNA and RNA recognition: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1169 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 195 S. Ill., graph. Darst. |
| ISBN: | 9781493908813 |
Internformat
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| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a RNA | |
| 650 | 4 | |a DNA | |
| 650 | 4 | |a Immune recognition / Laboratory manuals | |
| 650 | 4 | |a Nucleic acids / Laboratory manuals | |
| 650 | 4 | |a Cell receptors / Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804152457223208960 |
|---|---|
| adam_text | Titel: Innate DNA and RNA recognition
Autor: Anders, Hans-Joachim
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... ix
Part I Analysis of Viral Nucleic Acid Sensing
In-Silico and In-Vitro
1 Detection of RNA Modifications by HPLC Analysis
and Competitive ELISA......................................... 3
Gemot Nees, Andreas Kaufmann, and Stefan Bauer
2 Enzymatic Synthesis and Purification of a Defined RIG-I Ligand........... 15
Marion Goldeck, Martin Schlee, Gunther Hartmann,
and Veit Hornung
3 Crystallization of Mouse RIG-I ATPase Domain: In Situ Proteolysis........ 27
Filiz Civril and Karl-Peter Hopf ner
4 Isolation of RIG-I-Associated RNAs from Virus-Infected Cells............ 37
Andreas Schmidt, Andreas Linder, Nicolas Linder,
and Simon Kothenfusser
5 Structure Modeling of Toll-Like Receptors........................... 45
fing Gong and Tiandi Wei
6 Nucleic Acid Recognition in Dendritic Cells.......................... 55
Alexander Heiseke, Katharina Eisendcher, and Anne Krug
7 Viral Nucleic Acid Recognition in Human Nonimmune Cells:
In Vitro Systems............................................... 67
Andrea Ribeiro and Markus W örnle
8 Analysis of Nucleic Acid-Induced Nonimmune Cell Death In Vitro......... 77
Simone Romoli and Adriana Migliorini
9 In Vitro Analysis of Nucleic Acid Recognition in B Lymphocytes........... 87
Saskia Ziegler and Isabelle Bekeredjian-Ding
10 Mapping of Optimal CD8 T Cell Epitopes........................... 97
Julia Roider, Thomas Vollbrecht, and Rika Draenert
11 A Modular Approach to Suppression Assays: TLR Ligands,
Conditioned Medium, and Viral Infection............................ 107
Viktor H. Koelzer and David Anz
12 MicroRNA Methodology: Advances in miRNA Technologies............. 121
Theresa Kaeuferle, Sabine Bartel, Stefan Dehmel,
and Susanne Krauss-Etschmann
vii
viii Contents
Part II Analysis of Nucleic Acid Sensing In-Vivo
13 Expression Profiling by Real-Time Quantitative Polymerase
Chain Reaction (RT-qPCR)....................................... 133
Maciej Lech and Hans-Joachim Anders
14 Evaluating the Role of Nucleic Acid Antigens in Murine Models
of Systemic Lupus Erythematosus.................................. 143
Amanda A. Watkins, Ramon G.B. Bonegio, and Ian R. Rifkin
15 Induction and Analysis of Nephrotoxic Serum Nephritis in Mice........... 159
John M. Hoppe and Volker Vielhauer
16 Isolation of Intratumoral Leukocytes of TLR-Stimulated
Tumor-Bearing Mice............................................ 175
Moritz Rapp, David Anz, and Max Schnurr
17 Bifunctional siRNAs for Tumor Therapy............................. 181
Fanny Matheis and Robert Besch
Index................................................................ 193
|
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| id | DE-604.BV042034479 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:11:01Z |
| institution | BVB |
| isbn | 9781493908813 |
| language | English |
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| physical | XI, 195 S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Innate DNA and RNA recognition methods and protocols ed. by Hans-Joachim Anders ... New York, NY [u.a.] Humana Press 2014 XI, 195 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1169 Includes bibliographical references and index RNA DNA Immune recognition / Laboratory manuals Nucleic acids / Laboratory manuals Cell receptors / Laboratory manuals Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf Antigen (DE-588)4002272-9 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 s Antigen (DE-588)4002272-9 s Labortechnik (DE-588)4123602-6 s b DE-604 Anders, Hans-Joachim Sonstige oth Methods in molecular biology 1169 (DE-604)BV035362695 1169 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027475889&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Innate DNA and RNA recognition methods and protocols Methods in molecular biology RNA DNA Immune recognition / Laboratory manuals Nucleic acids / Laboratory manuals Cell receptors / Laboratory manuals Nucleinsäuren (DE-588)4172117-2 gnd Antigen (DE-588)4002272-9 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4172117-2 (DE-588)4002272-9 (DE-588)4123602-6 |
| title | Innate DNA and RNA recognition methods and protocols |
| title_auth | Innate DNA and RNA recognition methods and protocols |
| title_exact_search | Innate DNA and RNA recognition methods and protocols |
| title_full | Innate DNA and RNA recognition methods and protocols ed. by Hans-Joachim Anders ... |
| title_fullStr | Innate DNA and RNA recognition methods and protocols ed. by Hans-Joachim Anders ... |
| title_full_unstemmed | Innate DNA and RNA recognition methods and protocols ed. by Hans-Joachim Anders ... |
| title_short | Innate DNA and RNA recognition |
| title_sort | innate dna and rna recognition methods and protocols |
| title_sub | methods and protocols |
| topic | RNA DNA Immune recognition / Laboratory manuals Nucleic acids / Laboratory manuals Cell receptors / Laboratory manuals Nucleinsäuren (DE-588)4172117-2 gnd Antigen (DE-588)4002272-9 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | RNA DNA Immune recognition / Laboratory manuals Nucleic acids / Laboratory manuals Cell receptors / Laboratory manuals Nucleinsäuren Antigen Labortechnik |
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