Establishment of in vitro cultures of porcine reproductive epithelia: oviduct and cervix uteri
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2014
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals |
| Beschreibung: | IV, 73 S. Ill., graph. Darst. |
| ISBN: | 9783863875091 |
Internformat
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| 245 | 1 | 0 | |a Establishment of in vitro cultures of porcine reproductive epithelia |b oviduct and cervix uteri |c vorgelegt von Katrin Mießen |
| 246 | 1 | 3 | |a Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2014 | |
| 300 | |a IV, 73 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals | ||
| 502 | |a Berlin, Freie Univ., Diss., 2014 | ||
| 650 | 7 | |a pigs |2 cabt | |
| 650 | 7 | |a oviducts |2 cabt | |
| 650 | 7 | |a cervix |2 cabt | |
| 650 | 7 | |a epithelium |2 cabt | |
| 650 | 7 | |a cell culture |2 cabt | |
| 650 | 7 | |a differentiation |2 cabt | |
| 650 | 7 | |a polarization |2 cabt | |
| 650 | 7 | |a long term experiments |2 cabt | |
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| 650 | 0 | 7 | |a Schwein |0 (DE-588)4053848-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Epithelzelle |0 (DE-588)4259648-8 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
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| adam_text | CONTENTS
LIST OF TABLES IV
LIST OF FIGURES IV
1 INTRODUCTION 1
1.1 THE PORCINE FEMALE REPRODUCTIVE TRACT 1
1.1.1 EPITHELIAL CELLS 2
1.1.2 THE PORCINE OESTRUS CYCLE 3
1.1.3 EPITHELIA OF THE PORCINE REPRODUCTIVE TRACT 5
1.2 CELL CULTURE 6
1.2.1 HISTORY AND TERMINOLOGY 6
PRIMARY CULTURE 7
CELL LINE 7
PASSAGING 7
1.2.2 EPITHELIAL CELL CULTURE SYSTEMS IN REPRODUCTIVE RESEARCH .... 7
OVIDUCTAL EPITHELIUM 8
CERVICAL EPITHELIUM 11
1.2.3 DEMANDS ON THE EPITHELIAL IN VITRO MODEL SYSTEMS OF OVIDUCT
AND CERVIX UTERI 12
MAINTENANCE OF THE IN VIVO PHENOTYPE 12
OPTIMIZED CULTURE CONDITIONS 13
AVAILABILITY OF THE NATIVE TISSUE 13
1.3 SCIENTIFIC QUESTION 14
I
HTTP://D-NB.INFO/1054308446
CONTENTS
2 PAPER I: 16
2.1 MIESSEN. K.. SHARBATI. S.. EINSPANIER, R. AND SCHOEN, J. (2011).
MODELLING THE PORCINE OVIDUCT EPITHELIUM: A POLARIZED IN VITRO SYSTEM
SUITABLE FOR LONG-TERM CULTIVATION.
THERIOGENOLOGY, 76(5):900-910 16
3 PAPER II: 28
3.1 MIESSEN. K.. EINSPANIER, R. AND SCHOEN, J. (2012).
ESTABLISHMENT AND CHARACTERIZATION OF A DIFFERENTIATED EPITHELIAL CELL
CULTURE MODEL DERIVED FROM THE PORCINE CERVIX UTERI.
BMC VETERINAR RESEARCH. 8:31 28
4 DISCUSSION 39
OVIDUCTAL CELL CULTURE 39
CERVICAL CELL CULTURE 41
4.1 DISCUSSION OF METHODS 45
4.1.1 MODEL SYSTEMS 45
4.1.2 CELL CULTURE 45
4.1.3 CELL LINES VERSUS PRIMARY CELLS 47
4.1.4 CELL ISOLATION OF CERVICAL EPITHELIAL CELLS 49
4.1.5 EVALUATION OF GROWTH MEDIA 49
4.1.6 SPECIFIC GROWTH CONDITIONS 51
4.1.7 THE PIG AS A MODEL IN RESEARCH FOR HUMAN REPRODUCTION ... 51
5 OUTLOOK 54
6 SUMMARY 56
7 ZUSAMMENFASSUNG 58
BIBLIOGRAPHY 60
A APPENDIX 71
II
CONTENTS
A.L NACHDRUCKGENEHMIGUNGEN 71
A.2 DANKSAGUNG 72
A.3 SELBSTSTANDIGKEITSERKLARUNG 73
III
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| id | DE-604.BV042001863 |
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| indexdate | 2024-07-10T01:10:18Z |
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| isbn | 9783863875091 |
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| spelling | Mießen, Katrin Verfasser (DE-588)1054406820 aut Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri vorgelegt von Katrin Mießen Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals Berlin Mensch und Buch Verl. 2014 IV, 73 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals Berlin, Freie Univ., Diss., 2014 pigs cabt oviducts cabt cervix cabt epithelium cabt cell culture cabt differentiation cabt polarization cabt long term experiments cabt Eileiter (DE-588)4151214-5 gnd rswk-swf Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf In-vitro-Kultur (DE-588)4332883-0 gnd rswk-swf Gebärmutterhals (DE-588)4156124-7 gnd rswk-swf Schwein (DE-588)4053848-5 gnd rswk-swf Epithelzelle (DE-588)4259648-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Schwein (DE-588)4053848-5 s Gebärmutterhals (DE-588)4156124-7 s Eileiter (DE-588)4151214-5 s Epithelzelle (DE-588)4259648-8 s Zelldifferenzierung (DE-588)4067536-1 s In-vitro-Kultur (DE-588)4332883-0 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000097164-4 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000097164 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027443881&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mießen, Katrin Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri pigs cabt oviducts cabt cervix cabt epithelium cabt cell culture cabt differentiation cabt polarization cabt long term experiments cabt Eileiter (DE-588)4151214-5 gnd Zelldifferenzierung (DE-588)4067536-1 gnd In-vitro-Kultur (DE-588)4332883-0 gnd Gebärmutterhals (DE-588)4156124-7 gnd Schwein (DE-588)4053848-5 gnd Epithelzelle (DE-588)4259648-8 gnd |
| subject_GND | (DE-588)4151214-5 (DE-588)4067536-1 (DE-588)4332883-0 (DE-588)4156124-7 (DE-588)4053848-5 (DE-588)4259648-8 (DE-588)4113937-9 |
| title | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri |
| title_alt | Etablierung porciner in vitro-Zellkulturen aus Eileiter und Gebärmutterhals |
| title_auth | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri |
| title_exact_search | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri |
| title_full | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri vorgelegt von Katrin Mießen |
| title_fullStr | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri vorgelegt von Katrin Mießen |
| title_full_unstemmed | Establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri vorgelegt von Katrin Mießen |
| title_short | Establishment of in vitro cultures of porcine reproductive epithelia |
| title_sort | establishment of in vitro cultures of porcine reproductive epithelia oviduct and cervix uteri |
| title_sub | oviduct and cervix uteri |
| topic | pigs cabt oviducts cabt cervix cabt epithelium cabt cell culture cabt differentiation cabt polarization cabt long term experiments cabt Eileiter (DE-588)4151214-5 gnd Zelldifferenzierung (DE-588)4067536-1 gnd In-vitro-Kultur (DE-588)4332883-0 gnd Gebärmutterhals (DE-588)4156124-7 gnd Schwein (DE-588)4053848-5 gnd Epithelzelle (DE-588)4259648-8 gnd |
| topic_facet | pigs oviducts cervix epithelium cell culture differentiation polarization long term experiments Eileiter Zelldifferenzierung In-vitro-Kultur Gebärmutterhals Schwein Epithelzelle Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000097164 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027443881&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mießenkatrin establishmentofinvitroculturesofporcinereproductiveepitheliaoviductandcervixuteri AT mießenkatrin etablierungporcinerinvitrozellkulturenauseileiterundgebarmutterhals |