Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache |
| Beschreibung: | 112 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |c von Ying Wang |
| 246 | 1 | 3 | |a Immunologische und periphere endogene Opioidmechanismen bei Analgesie durch Elektroakupunktur |
| 264 | 1 | |c 2014 | |
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Datensatz im Suchindex
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| adam_text | IMMUNE AND PERIPHERAL ENDOGENOUS OPIOID MECHANISMS OF
ELECTROACUPUNCTURE ANALGESIA
IMMUNOLOGISCHE UND PERIPHERE ENDOGENE OPIOIDMECHANISMEN BEI
ANALGESIE DURCH ELEKTROAKUPUNKTUR
DOCTORAL THESIS FOR A DOCTORAL DEGREE
AT THE GRADUATE SCHOOL OF LIFE SCIENCES,
JULIUS-MAXIMILIANS-UNIVERSITAT WIIRZBURG,
SECTION.
..
N EUR
OSCIENCE
SUBMITTED BY
VING WANG
FROM
XI AN, CHINA
WIIRZBURG
2014
HTTP://D-NB.INFO/1054939128
CONTENTS
ABBREVIATIONS 1
SUMMARY 5
ZUSAMMENFASSUNG 8
1. INTRODUCTION 11
1.1 OPIOID-DEPENDENT ACUPUNCTURE ANALGESIA (AA) 11
1.2 COMPARISON OF DIFFERENT MODES OF ELECTROACUPUNCTURE (EA) TREATMENT
12
1.3 TONIC
ANALGESIA MEDIATED BY PERIPHERAL OPIOID PEPTIDES CONTAINING-IMMUNE CELLS
14
1.4 CYTOKINE-MEDIATED HYPERALGESIC AND ANTI-HYPERALGESIC RESPONSES IN
PAIN 17
1.5 AIMS 18
2. MATERIALS AND METHODS 19
2.1 MATERIALS 19
2.1.1 CHEMICALS 19
2.1.2 ANTIBODIES (ABS) 21
2.1.3 ADDITIONAL
DRUGS FOR PERIPHERAL ADMINISTRATION 23
2.1.4 KITS 23
2.1.5 EXPERIMENTAL SOFTWARE
AND HARDWARE 24
2.2 EXPERIMENTAL METHODS 26
2.2.1 PERIPHERAL INFLAMMATORY PAIN MODEL ON ANIMALS 26
2.2.2 ESTABLISHMENT OF EA TREATMENT 26
2.2.3 INSTITUTION OF COMPUTER-BASED THREE-DIMENSIONAL (3D) RAT MODEL 29
2.2.4 DRUG DELIVERY 30
2.2.5 MEASUREMENT OF NOCICEPTIVE THRESHOLDS (BEHAVIORAL ASSAYS) 30
2.2.6 MEASUREMENT OF PAW TEMPERATURE AND VOLUME 34
2.2.7 CYTOKINE ARRAY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) 34
2.2.8 RNA
EXTRACTION, CDNA TRANSCRIPTION AND REVERSE TRANSCRIPTION-POLYMERASE
CHAIN
REACTION (RT-PCR) 36
2.2.9 IMMUNOHISTOCHEMISTRY STAINING (IHC) 38
2.2.10 FLUORESCENCE-ACTIVATED CELL SORTING (FACS) 40
2.2.11 WESTERN BLOT (WB) 41
2.2.12 RAT MACROPHAGES HARVEST AND STIMULATION BY CXCL10 (RELEASE) 44
2.3 EXPERIMENTAL DESIGN 45
2.4 STATISTICAL ANALYSIS 46
3. RESULTS 47
3.1 ACUPOINT POSITIONING AND NEEDLING WERE MODELED BY COMPUTER-BASED 3D
RAT MODEL ...47
3.2 OPTIMAL PARAMETER SELECTION FOR EA BY COMPARISON OF DIFFERENT
FREQUENCIES 48
3.3 EA PRODUCED SUSTAINED ANTINOCICEPTION AND ANTI-INFLAMMATION 50
3.4 PERIPHERAL ENDOGENOUS OPIOIDS
CONTRIBUTED TO TONIC ANTINOCICEPTION OF EA 52
3.5 EA SELECTIVELY REGULATED EXPRESSIONS OF CERTAIN CYTOKINES/CHEMOKINES
56
3.6 UPREGULATION OF CXCL10 AND CXCR3-EXPRESSING MACROPHAGES WERE
ASSOCIATED WITH
EA 58
3.7 EA INCREASED OPIOID PEPTIDES EXPRESSION ON RECRUITED
MONOCYTES/MACROPHAGES 63
3.8 CXCL10 REVERSED CFA-INDUCED MECHANICAL HYPERALGESIA VIA PERIPHERAL
OPIOID
PEPTIDES 68
3.9 CXCL10 ENHANCED OPIOID PEPTIDES EXPRESSION ON ACCUMULATED
MONOCYTES/MACROPHAGES IN VIVO INSTEAD OF TRIGGERING OPIOID PEPTIDE
RELEASE IN VITRO 70
3.10 BLOCKAGE OF CXCL10 IMPAIRED EA-INDUCE ANTINOCICEPTION AND
DOWNREGULATED OPIOID
PEPTIDE EXPRESSION ON MONOCYTES/MACROPHAGES 74
3.11 EXPRESSION OF OPIOID RECEPTORS WAS NOT ALTERED BY EA 80
4. DISCUSSION 82
4.1 3D MODELED ACUPUNCTURE NEEDLING AND EA TREATMENT ON CONSCIOUS FULLY
FREE-MOVING
RATS 82
4.2 CHARACTERISTICS OF ACUPUNCTURE-INDUCED ANALGESIC AND
ANTI-INFLAMMATORY MECHANISMS
84
4.2.1 CHARACTERISTICS OF ENDOGENOUS OPIOIDS ASSOCIATED ANALGESIC
MECHANISMS IN AA... 84
4.2.2 ENDOGENOUS CYTOKINES-RELATED ANTI-INFLAMMATORY PROPERTIES OF
ACUPUNCTURE 86
4.2.3 POTENTIAL PLACEBO EFFECT UNDERLYING VERUM ACUPUNCTURE REQUIRES
SHAM ACUPUNCTURE
CONTROL 87
4.3 TANGLED INTERACTION BETWEEN IFN-GAMMA AND CXCL10 IN IMMUNE RESPONSES
AND
POTENTIAL ASSOCIATION IN ATTENUATING INFLAMMATORY PAIN BY EA 87
4.4 BILATERAL ROLE OF CHEMOKINES IN REGULATING PAIN AND NOVEL
ANTINOCICEPTIVE PROPERTY OF
CXCL10 IN RELIEVING INFLAMMATORY PAIN BY EA 90
5. REFERENCES 95
CURRICULUM VITAE 106
PUBLICATIONS 109
APPENDIX ILL
UNITS ILL
COPYRIGHT LICENSES 112
|
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| spelling | Wang, Ying Verfasser (DE-588)1053809719 aut Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia von Ying Wang Immunologische und periphere endogene Opioidmechanismen bei Analgesie durch Elektroakupunktur 2014 112 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2014 Chemokin CXCL10 (DE-588)7758346-2 gnd rswk-swf Endorphine (DE-588)4152186-9 gnd rswk-swf Cytokine (DE-588)4205701-2 gnd rswk-swf Ratte (DE-588)4177011-0 gnd rswk-swf Elektroakupunktur (DE-588)4014232-2 gnd rswk-swf Analgesie (DE-588)4001847-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Elektroakupunktur (DE-588)4014232-2 s Chemokin CXCL10 (DE-588)7758346-2 s Endorphine (DE-588)4152186-9 s Cytokine (DE-588)4205701-2 s Analgesie (DE-588)4001847-7 s Ratte (DE-588)4177011-0 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-98326 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-98326 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027422741&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Ying Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia Chemokin CXCL10 (DE-588)7758346-2 gnd Endorphine (DE-588)4152186-9 gnd Cytokine (DE-588)4205701-2 gnd Ratte (DE-588)4177011-0 gnd Elektroakupunktur (DE-588)4014232-2 gnd Analgesie (DE-588)4001847-7 gnd |
| subject_GND | (DE-588)7758346-2 (DE-588)4152186-9 (DE-588)4205701-2 (DE-588)4177011-0 (DE-588)4014232-2 (DE-588)4001847-7 (DE-588)4113937-9 |
| title | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |
| title_alt | Immunologische und periphere endogene Opioidmechanismen bei Analgesie durch Elektroakupunktur |
| title_auth | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |
| title_exact_search | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |
| title_full | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia von Ying Wang |
| title_fullStr | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia von Ying Wang |
| title_full_unstemmed | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia von Ying Wang |
| title_short | Immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |
| title_sort | immune and peripheral endogenous opioid mechanisms of electroacupuncture analgesia |
| topic | Chemokin CXCL10 (DE-588)7758346-2 gnd Endorphine (DE-588)4152186-9 gnd Cytokine (DE-588)4205701-2 gnd Ratte (DE-588)4177011-0 gnd Elektroakupunktur (DE-588)4014232-2 gnd Analgesie (DE-588)4001847-7 gnd |
| topic_facet | Chemokin CXCL10 Endorphine Cytokine Ratte Elektroakupunktur Analgesie Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-98326 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027422741&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wangying immuneandperipheralendogenousopioidmechanismsofelectroacupunctureanalgesia AT wangying immunologischeundperiphereendogeneopioidmechanismenbeianalgesiedurchelektroakupunktur |