Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
VVB Laufersweiler Verlag
2013
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 141 S. Ill., graph. Darst. |
| ISBN: | 9783835960541 |
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| 245 | 1 | 0 | |a Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis |c eingereicht von Nisha Balsara |
| 264 | 1 | |a Gießen |b VVB Laufersweiler Verlag |c 2013 | |
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Datensatz im Suchindex
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| adam_text | I TABLE OF CONTENTS
I TABLE OF CONTENTS 5
II LIST OF FIGURES 8
III LIST OF TABLES 10
IV LIST OF ABBREVIATIONS 11
V SUMMARY 19
VI ZUSAMMENFAS5UNG 21
1 INTRODUCTION 23
1.1 DIFFUSE PARENCHYMA) LUNG DISEASES 23
1.2 IDIOPATHIC PULMONARY FIBROSIS 24
1.2.1 DEFINITION AND EPIDEMIOLOGY 24
1.2.2 CLINICAL FEATURES, RADIOLOGICAL CHARACTERISTICS AND THERAPY
OPTIONS 26
1.2.3 HISTOPATHOLOGICAL CHARACTERISTICS 27
1.2.4 PATHOMCCHANISM OF IDIOPATHIC PULMONARY FIBROSIS 27
1.3 WNT SIGNALLING 31
1.3.1 THE CANONICAL WNT/P-CATENIN SIGNALLING PATHWAY 31
1.4 WNT SIGNALLING IN THE LUNG 34
1.4.1 LUNG DEVELOPMENT AND HOMEOSTASIS 34
1.4.2 LUNG CANCER 36
1.5 WNT SIGNALLING IN FIBROTIC DISORDERS 37
2 AIMS OF THE STUDY 38
3 MATERIALS AND METHODS 39
3.1 MATERIALS 39
3.1.1 EQUIPMENT 39
3.1.2 CHEMICAL REAGENTS 40
3.1.3 ANTIBODIES 42
3.1.4 RECOMBINANT PROTEINS 43
3.1.5 HUMAN TISSUES 43
3.1.6 ANIMAL TISSUES 44
3.1.7 PRIMARY CELLS 44
3.2 METHODS 44
3.2.1 ANIMAL MODEL OF BLCOMYCIN-INDUCCD PULMONARY FIBROSIS IN MICE 44
3.2.2 WNT REPORTER MICE (TOPGAL) 45
3.2.3 ISOLATION OF PRIMARY MOUSE ALVEOLAR EPITHELIAL TYPE II (ATII)
CELLS 45
3.2.4 SUPEMATANTS 48
5
HTTP://D-NB.INFO/1037039610
3.2.5 RNA ISOLATION AND MEASUREMENT 48
3.2.5.1 RNA ISOLATION FROM LUNG HOMOGENATES 48
3.2.5.2 RNA ISOLATION FROM CULTURED CELLS 48
3.2.6 CDNA SYNTHESIS 49
3.2.7 QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION (QRT-PCR) 50
3.2.8 DNA AGAROSE GEL ELECTROPHORESIS 52
3.2.9 IMMUNOHISTOCHEMISTRY 52
3.2.10 IMMUNOCYTOCHEMISTRY 54
3.2.11 WESTERN BLOT ANALYSIS 55
3.2.12 [
1
H]-DIYMIDINE PROLIFERATION ASSAY 57
3.2.13 WHOLE GENOME MICROARRAY 58
3.2.14 ENZYME LINKED-IMMUNO-SORBENT ASSAY (EL1SA) 58
3.2.15 STATISTICAL ANALYSIS 59
4 RESULTS 60
4.1 ANALYSIS OF THE CANONICAL WNT/0-CATENIN SIGNALLING PATHWAY IN
IDIOPATHIC PULMONARY
FIBROSIS 60
4.1.1 EXPRESSION OF THE CANONICAL WNT/JJ-CATENIN SIGNALLING PATHWAY IN
IDIOPATHIC
PULMONARY FIBROSIS 60
4.1.2 LOCALISATION OF THE CANONICAL WNT/{3-CATCNIN SIGNALLING PATHWAY IN
IDIOPATHIC
PULMONARY FIBROSIS 63
4.1.3 ACTIVITY OF THE CANONICAL WNT/FJ-CATENIN SIGNALLING PATHWAY IN
IDIOPATHIC PULMONARY
FIBROSIS 72
4.2 ANALYSIS OF THE CANONICAL WNT/F)-CATCNIN SIGNALLING PATHWAY IN
EXPERIMENTAL PULMONARY
FIBROSIS 74
4.2.1 EXPRESSION OF THE CANONICAL WNT/FI-CATENIN SIGNALLING PATHWAY IN
EXPERIMENTAL
PULMONARY FIBROSIS 74
4.2.2 LOCALISATION OF THE WNT/P-CATENIN SIGNALLING PATHWAY IN
EXPERIMENTAL PULMONARY
FIBROSIS 77
4.3 FUNCTIONAL ANALYSIS OF THE WNT/P ATENIN SIGNALLING PATHWAY 87
4.3.1 PURITY AND PHENOTYPE OF PRIMARY MOUSE ALVEOLAR EPITHELIAL TYPE II
(ATII) CELLS 88
4.3.2 EXPRESSION OF WNT3A-INDUCED TARGET GENES IN PRIMARY MOUSE ALVEOLAR
EPITHELIAL TYPE
11 (AT11) CELLS 91
4.3.3 PROLIFERATIVE EFFECTS OF WNT3A STIMULATION ON PRIMARY MOUSE
ALVEOLAR EPITHELIAL TYPE
11 (ATII) CELLS 92
4.3.4 WHOLE GENOME MICROARRAY ANALYSIS ON WNI3A-INDUCED PRIMARY MOUSE
ALVEOLAR
EPITHELIAL TYPE II (ATII) CELLS 94
4.4 ANALYSIS OF THE NOVEL WNT TARGET GENES ILIP AND IL6 IN VITRO AND IN
VIVO 98
4.4.1 ELISA ANALYSIS FOR QUANTIFICATION OF! LI (5 AND IL6 98
5 DISCUSSION 103
6
5. I CONCLUSIONS AND FUTURE PERSPECTIVES 116
6 APPENDIX 118
6.1 LIST OF HUMAN (Q)RT-PCR PRIMERS 118
6.2 LIST OF MOUSE (Q)RT-PCR PRIMERS 121
6.3 CHARACTERISTICS OF IPF PATIENTS 124
6.4 LIST OF ANTIBODIES 125
6.4.1 PRIMARY ANTIBODIES 125
6.4.2 SECONDARY ANTIBODIES 126
6.5 MICROARRAY GENE LIST 127
7 REFERENCES 129
8 DECLARATION 139
9 ACKNOWLEDGEMENTS 140
7
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| any_adam_object | 1 |
| author | Balsara, Nisha |
| author_GND | (DE-588)1049481615 |
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| bvnumber | BV041933403 |
| ctrlnum | (OCoLC)915488082 (DE-599)DNB1037039610 |
| dewey-full | 616.24 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.24 |
| dewey-search | 616.24 |
| dewey-sort | 3616.24 |
| dewey-tens | 610 - Medicine and health |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T01:08:40Z |
| institution | BVB |
| isbn | 9783835960541 |
| language | English |
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| physical | 141 S. Ill., graph. Darst. |
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| spelling | Balsara, Nisha Verfasser (DE-588)1049481615 aut Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis eingereicht von Nisha Balsara Gießen VVB Laufersweiler Verlag 2013 141 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Gießen, Univ., Diss., 2013 lungs cabt fibrosis cabt experiments cabt signals cabt Lungenfibrose (DE-588)4168315-8 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Wnt-Proteine (DE-588)7515809-7 gnd rswk-swf Pathogenese (DE-588)4115512-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Lungenfibrose (DE-588)4168315-8 s Pathogenese (DE-588)4115512-9 s Wnt-Proteine (DE-588)7515809-7 s Signaltransduktion (DE-588)4318717-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027376637&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Balsara, Nisha Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis lungs cabt fibrosis cabt experiments cabt signals cabt Lungenfibrose (DE-588)4168315-8 gnd Signaltransduktion (DE-588)4318717-1 gnd Wnt-Proteine (DE-588)7515809-7 gnd Pathogenese (DE-588)4115512-9 gnd |
| subject_GND | (DE-588)4168315-8 (DE-588)4318717-1 (DE-588)7515809-7 (DE-588)4115512-9 (DE-588)4113937-9 |
| title | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis |
| title_auth | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis |
| title_exact_search | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis |
| title_full | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis eingereicht von Nisha Balsara |
| title_fullStr | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis eingereicht von Nisha Balsara |
| title_full_unstemmed | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis eingereicht von Nisha Balsara |
| title_short | Role of the WNT/b-catenin signal pathway in idiopathic and experimental pulmonary fibrosis |
| title_sort | role of the wnt b catenin signal pathway in idiopathic and experimental pulmonary fibrosis |
| topic | lungs cabt fibrosis cabt experiments cabt signals cabt Lungenfibrose (DE-588)4168315-8 gnd Signaltransduktion (DE-588)4318717-1 gnd Wnt-Proteine (DE-588)7515809-7 gnd Pathogenese (DE-588)4115512-9 gnd |
| topic_facet | lungs fibrosis experiments signals Lungenfibrose Signaltransduktion Wnt-Proteine Pathogenese Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027376637&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT balsaranisha roleofthewntbcateninsignalpathwayinidiopathicandexperimentalpulmonaryfibrosis |