Fox transcription factors in adult neurogenesis:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20140505-1185841-0-4 Inhaltsverzeichnis |
| Beschreibung: | XVI, 134 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | TECHNISCHE UNIVERSITAT MUNCHEN
FAKULTAT WISSENSCHAFTSZENTRUM WEIHENSTEPHAN FUR ERNAHRUNG,
LANDNUTZUNG UND UMWELT
LEHRSTUHL FUR ENTWICKLUNGSGENETIK
FOX TRANSCRIPTION FACTORS IN ADULT NEUROGENESIS
IRIS SCHAFFNER
VOLLSTANDIGER
ABDRUCK DER VON DER FAKULTAT WISSENSCHAFTSZENTRUM
WEIHENSTEPHAN
FUR ERNAHRUNG, LANDNUTZUNG
UND UMWELT DER TECHNISCHEN
UNIVERSITAT MUNCHEN
ZUR
ERLANGUNG DES AKADEMISCHEN
GRADES EINES
DOKTORS DER NATURWISSENSCHAFTEN
GENEHMIGTEN DISSERTATION.
VORSITZENDER: UNIV.-PROF. DR.
S. SCHERER
PRUFER DER DISSERTATION: 1. UNIV.-PROF.
DR. W. WURST
2. UNIV.-PROF. DR.
D. C. LIE,
FRIEDRICH-ALEXANDER-UNIVERSITAT ERLANGEN-NOMBERG
DIE DISSERTATION WURDE AM 08.01.2014 BEI DER TECHNISCHEN UNIVERSITAT
MUNCHEN
EINGEREICHT UND DURCH DIE FAKULTAT WISSENSCHAFTSZENTRUM WEIHENSTEPHAN
FUR
ERNAHRUNG, LANDNUTZUNG UND
UMWELT AM 19.02.2014 ANGENOMMEN.
HTTP://D-NB.INFO/1053778805
XIII
V TABLE OF CONTENTS
I ABSTRACT V
II ZUSAMMENFASSUNG VII
III ABBREVIATIONS IX
IV LIST OF FIGURES XII
V TABLE OF CONTENTS XIII
1 INTRODUCTION 1
1.1 NEUROGENIC NICHES IN THE ADULT BRAIN 1
1.1.1 ADULT HIPPOCAMPAL NEUROGENESIS 2
1.1.2 ADULT SUBVENTRICULAR ZONE NEUROGENESIS 5
1.1.3 FUNCTIONAL RELEVANCE AND SIGNIFICANCE OF ADULT NEUROGENESIS IN
HEALTH AND DISEASE... 6
1.1.4 REGULATION OF ADULT NEURAL STEM CELL HOMEOSTASIS 8
1.2 FOX TRANSCRIPTION FACTORS 12
1.2.1 FOXO TRANSCRIPTION FACTORS 13
1.2.2 FOXG1 TRANSCRIPTION FACTOR 15
1.3 AIMS OF THE STUDY 18
2 RESULTS 19
2.1 FOXO TRANSCRIPTION FACTORS IN ADULT NEUROGENESIS 19
2.1.1 INCREASE IN PROLIFERATION AND NEUROGENESIS IN FOX01/3/4 DEFICIENT
ADULT NEURAL STEM CELLS IN VITRO 20
2.1.2 CHANGED SURVIVAL, PROLIFERATION AND NEUROGENESIS OF FOX01/3/4
KO CELLS IN VIVO 24
2.1.2.1 VALIDATION OF THE FOXOI/3/4 KO MOUSE MODEL 24
2.1.2.2 LOSS OF (3-GAL
POSITIVE ADULT RECOMBINED CELLS OVER TIME
IN THE DENTATE GYRUS OF FOXOI/3/4 KO ANIMALS 26
2.1.2.3 IMMEDIATE INCREASE IN PROLIFERATION AND NEUROGENESIS IN
FOX01/3/4 DEFICIENT ANSCS IN THE DENTATE GYRUS IN VIVO...27
2.1.2.4 SLIGHTLY ONGOING INCREASE IN PROLIFERATION AND
NEUROGENESIS 28 AFTER FOX01/3/4 KO INDUCTION 30
XIV
2.1.3 SMAD PATHWAYS AS POSSIBLE SIGNALING PATHWAYS CONTROLLING
FOXOS 33
2.1.3.1 ACTIVE SMAD SIGNALING IN FOX01/3/4 DEFICIENT CELLS
IN VIVO 33
2.1.3.-2 SMAD SIGNALING IS ABLE TO CONTROL FOX01/3/4 KO CELLS
IN VITRO 35
2.1.4 POSSIBLE DOWNSTREAM TARGETS OF FOXOI/3/4 TRANSCRIPTION FACTORS...
38
2.1.5 IMPAIRED DENDRITIC, SYNAPTIC AND MITOCHONDRIAL MORPHOLOGY OF
FOX01/3/4 DEPRIVED CELLS IN VIVO 42
2.1.5.1 MORPHOLOGY CHANGES IN 21-DAY-OLD NEWBORN NEURONS
DEFICIENT FOR FOX01/3/4 IN THE ADULT DENTATE GYRUS 43
2.1.5.2 MORPHOLOGY CHANGES IN 42-DAY-OLD NEWBORN NEURONS
DEFICIENT FOR FOX01/3/4 IN THE ADULT DENTATE GYRUS 47
2.2 TRANSCRIPTION FACTOR FOXG1 IN ADULT NEUROGENESIS 51
2.2.1 EXPRESSION OF FOXG1 IN THE ADULT DENTATE GYRUS 51
2.2.2 IMPAIRED SURVIVAL, CHANGES IN DIFFERENTIATION AND ABERRANT
MORPHOLOGY OF NEWBORN CELLS IN THE ADULT DG DUE TO OVER-
EXPRESSION OF AN ENDOGENOUS OR A DOMINANT-ACTIVE MUTANT
FORM OF FOXG1 IN VIVO 54
2.2.2.1 DECREASED SURVIVAL DUE TO FOXG1 GAIN OF FUNCTION 56
2.2.2.2 FOXG1 OVEREXPRESSION REDUCES NEUROGENESIS AND
INCREASES OLIGODENDROGENESIS 58
2.2.2.3 FOXG1 OVER-ACTIVITY RESULTS IN ABERRANT MORPHOLOGY 60
2.2.3 NO PHENOTYPIC CHANGES IN NEWBORN CELLS IN THE ADULT SVZ AFTER
OVER-EXPRESSION OF AN ENDOGENOUS OR A DOMINANT-ACTIVE
MUTATED FORM OF FOXG1 IN VIVO 62
3 DISCUSSION 66
3.1 FOXO TRANSCRIPTION FACTORS IN STEM CELL MAINTENANCE AND NEUROGENESIS
IN THE ADULT BRAIN 66
3.2 FOXO TRANSCRIPTION FACTORS AND THEIR ROLE IN MORPHOLOGICAL
MATURATION
OF NEWBORN CELLS IN THE ADULT DENTATE GYRUS 72
3.3 FOXO DEFICIENCY AFFECTS THE MITOCHONDRIAL COMPARTMENT IN NEWBORN
NEURONS
- HINTS TOWARDS A NEW MECHANISM UNDERLYING FOXO DEPEN
DENT NEURONAL DEVELOPMENT 74
3.4 TRANSCRIPTION FACTOR FOXG1 - DOSAGE AND NICHE DEPENDENT REGULATOR
OF ADULT NEUROGENESIS? 76
3.5 FOXG1 AND THE CONGENITAL FORM OF RETT SYNDROME 78
3.6 CONCLUSIONS 80
XV
4 MATERIAL AND METHODS 81
4.1 MATERIAL 81
4.1.1 CHEMICALS AND REAGENTS 81
4.1.2 _ ENZYMES AND
ENZYME BUFFERS 82
4.1.3 ANTIBODIES 83
4.1.4 KITS 84
4.1.5 OLIGODEOXYNUCLEOTIDES 84
4.1.6 ORGANISMS 86
4.1.7 PLASMIDS 86
4.1.8 BUFFERS AND SOLUTIONS 87
4.1.9 OTHER MATERIAL 90
4.1.10
EQUIPMENT 91
4.2 METHODS 92
4.2.1 PLASMID PRODUCTION 92
4.2.2 MOLECULAR CLONING 92
4.2.3 RNA METHODS 94
4.2.4 CELL CULTURE 95
4.2.5 FACSORTING 99
4.2.6 RETROVIRUS PREPARATION 100
4.2.7 ANIMALS AND STEREOTACTIC INJECTIONS 101
4.2.8 TISSUE PROCEDURES 105
4.2.9 HISTOLOGY PROCEDURES 105
4.2.10
PHENOTYPING OF CELLS 106
4.2.11
MORPHOLOGY ANALYSIS 106
4.2.12 STATISTICS 107
5 REFERENCES 108
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| spelling | Schäffner, Iris Verfasser aut Fox transcription factors in adult neurogenesis Iris Schäffner 2014 XVI, 134 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2014 Mit einer Zsfassung in dt. Sprache (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20140505-1185841-0-4 http://mediatum.ub.tum.de/node?id=1185841 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20140505-1185841-0-4 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027331911&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Fox transcription factors in adult neurogenesis |
| title_auth | Fox transcription factors in adult neurogenesis |
| title_exact_search | Fox transcription factors in adult neurogenesis |
| title_full | Fox transcription factors in adult neurogenesis Iris Schäffner |
| title_fullStr | Fox transcription factors in adult neurogenesis Iris Schäffner |
| title_full_unstemmed | Fox transcription factors in adult neurogenesis Iris Schäffner |
| title_short | Fox transcription factors in adult neurogenesis |
| title_sort | fox transcription factors in adult neurogenesis |
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