Functional analysis of the N-terminal domain of SPA1:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Ausgabe: | [Mikrofiche-Ausg.] |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 100 S. Ill., graph. Darst. 21 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | 3. TABLE OF CONTENTS
1. ABSTRACT 7
2. ZUSAMMENFASSUNG 9
3. TABLE OF CONTENTS 11
4. INTRODUCTION 13
4.1. LIGHT RESPONSES IN ARABIDOPSIS 13
4.2. PHOTORECEPTORS AND LIGHT PERCEPTION 14
4.3. LIGHT SIGNALING DOWNSTREAM OF PHOTORECEPTORS 15
4.4. REGULATION OF FLOWERING TIME BY PHOTOPERIOD 18
4.5. STRUCTURAL FEATURES OF PROTEIN KINASES 20
4.6. THE SPA1 KINASE-LIKE DOMAIN 29
4.7. AIMS OF THE PRESENT WORK 30
5. RESULTS 1 30
5.1. IN SILICO ANALYSIS OF THE SPA1 N-TERMINAL DOMAIN 30
5.2. ROLE OF THE SPA1 N-TERMINAL DOMAIN IN THE CONTROL OF FLOWERING TIME
41
5.3. ROLE OF THE SPA1 N-TERMINAL DOMAIN IN SPA1-COP1 AND SPA1-CO
INTERACTIONS. 46
5.4. IN PLANTA INTERACTION OF THE SPA1 N-TERMINAL DOMAIN WITH CO 47
5.5. CO PROTEIN LEVELS 48
5.6. ROLE OF THE SPA1 N-TERMINAL DOMAIN IN LIGHT-GROWN SEEDLINGS 49
5.7. EFFECTS OF AMINO ACID EXCHANGES ON THE SPA1 KINASE-LIKE DOMAIN
STRUCTURE 55
5.8. ANALYSIS OF SPA1 PHOSPHORYLATION 57
6. DISCUSSION 62
6.1. THE SPA1 KINASE-LIKE DOMAIN IS A PSEUDO-KINASE OF GROUP G 62
6.2. THE N-TERMINAL DOMAIN OF SPA1 IS ESSENTIAL FOR THE CONTROL OF
FLOWERING TIME AND
SPA1-CO INTERACTION 63
6.3. THE SPA1 N-TERMINAL DOMAIN IS INVOLVED IN R LIGHT-SPECIFIC SEEDLING
PHOTOMORPHOGENESIS 65
6.4. THE SPA1 KINASE-LIKE DOMAIN STRUCTURE RATHER THAN ITS KINASE
ACTIVITY IS
IMPORTANT FOR ITS FUNCTIONS 65
7. MATERIALS AND METHODS 67
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7.1. IN SILICO ANALYSES 67
7.2. PLANT MATERIAL AND GROWTH CONDITIONS 68
7.3. CLONING 69
7.1. LUMIER 69
7.2. FLUORESCENCE MICROSCOPY 69
7.3. PROTEIN ISOLATION AND WESTERN BLOTTING 69
7.4. MASS SPECTROMETRY 70
7.4.1 IN GEL DIGESTION 70
7.4.2 LC-MS/MS OF IN GEL DIGESTED PROTEINS 70
7.4.3 DATABASE SEARCHING 71
7.5. PRIMERS 71
7.6. SEQUENCE ACCESSIONS 73
8. ACKNOWLEDGMENTS 73
9. REFERENCES 74
APPENDIX I. SPA FAMILY ALIGNMENT 85
APPENDIX II. LUMIER 94
APPENDIX III. MASS SPECTROMETRY ANALYSES SUMMARIES 95
10. ERKLARUNG 100
11. CURRICULUM VITAE 101
12
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| author | Dieterle, Stefan 1974- |
| author_GND | (DE-588)1046873822 |
| author_facet | Dieterle, Stefan 1974- |
| author_role | aut |
| author_sort | Dieterle, Stefan 1974- |
| author_variant | s d sd |
| building | Verbundindex |
| bvnumber | BV041887608 |
| ctrlnum | (OCoLC)881632868 (DE-599)DNB1047715791 |
| discipline | Biologie |
| edition | [Mikrofiche-Ausg.] |
| format | Thesis Microfilm Book |
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| indexdate | 2024-07-10T01:07:29Z |
| institution | BVB |
| language | English |
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| physical | 100 S. Ill., graph. Darst. 21 cm |
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| spelling | Dieterle, Stefan 1974- Verfasser (DE-588)1046873822 aut Functional analysis of the N-terminal domain of SPA1 vorgelegt von Stefan Dieterle [Mikrofiche-Ausg.] 2013 100 S. Ill., graph. Darst. 21 cm txt rdacontent h rdamedia he rdacarrier Köln, Univ., Diss., 2013 Mikroform-Ausgabe Mikrofiche-Ausg.: 1 Mikrofiche : 24x (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Dieterle, Stefan, 1974- Functional analysis of the N-terminal domain of SPA1 2013 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027331593&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dieterle, Stefan 1974- Functional analysis of the N-terminal domain of SPA1 |
| subject_GND | (DE-588)4113937-9 |
| title | Functional analysis of the N-terminal domain of SPA1 |
| title_auth | Functional analysis of the N-terminal domain of SPA1 |
| title_exact_search | Functional analysis of the N-terminal domain of SPA1 |
| title_full | Functional analysis of the N-terminal domain of SPA1 vorgelegt von Stefan Dieterle |
| title_fullStr | Functional analysis of the N-terminal domain of SPA1 vorgelegt von Stefan Dieterle |
| title_full_unstemmed | Functional analysis of the N-terminal domain of SPA1 vorgelegt von Stefan Dieterle |
| title_short | Functional analysis of the N-terminal domain of SPA1 |
| title_sort | functional analysis of the n terminal domain of spa1 |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027331593&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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