Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 109 Bl. Ill., graph. Darst. 29,5 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
LIST OF ABBREVIATIONS JJ.I
01 SUMMARY 01
ZUSAMMENFASSUNG 03
02 INTRODUCTION 05
ANDROGEN RECEPTOR 05
ANDROGEN RECEPTOR IN PROSTATE CANCER 09
NOVEL ANDROGEN RECEPTOR ANTAGONISTS 10
HEAT SHOCK PROTEINS IN ANDROGEN RECEPTOR FUNCTION AND PROSTATE CANCER..
13
HISTONE DEACETYLASES IN ANDROGEN RECEPTOR FUNCTION AND IN PROSTATE
CANCER 15
AIM OF THIS THESIS .17
03 METHODS 18
HORMONES, CHEMICALS AND PLASMIDS 18
CELL LINES AND CULTURE 18
PROSTATE TISSUE 19
SENESCENCE-ASSOCIATED P-GALACTOSIDASE (SA P-GAL) STAINING, 19
QUANTITATIVE REVERSE TRANSCRIPTION PCR (QRT-PCR) 20
REPORTER GENE ASSAY 2.1
MAMMALIAN-ONE-HYBRID ASSAY 21
CHROMATIN IMMUNOPRECIPITATION (CHIP) 2.1
TRANSLOCATION STUDIES. 23
CONFOCAL MICROSCOPY. 23
NUCLEAR IMPORT STUDIES 24
ACCEPTOR PHOTOBLEACHING FLUORESCENCE RESONANCE ENERGY TRANSFER
(ABFRET) .24
FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING (FRAP) 24
04 RESULTS 26
THE NATURAL ANTIANDROGEN AA INHIBITS AR TRANSACTIVATION IN DIFFERENT
CELL LINES 26
HTTP://D-NB.INFO/1054102945
TABLE OF CONTENTS
AA INHIBITS WT AND MUTANT AR TRANSCATIVATION 28
AA INFLUENCES AR TARGET GENE EXPRESSION IN HUMAN PCA CELLS 31
AA DECELERATES AR NUCLEAR TRANSLOCATION 33
HDAC INHIBITORS REDUCE AR NUCLEAR TRANSLOCATION AND INDUCE CELLULAR
SENESCENCE IN PCA CELLS 36
HSP90 INHIBITION REVERSES ANTAGONIST- BUT NOT AGONIST-MEDIATED NUCLEAR
TRANSLOCATION OF WT AND MUTANT AR 39
AA PREVENTS AR N/C-TERMINAL INTERACTION AND REDUCES THE AGONIST-INDUCED
INTRANUCLEAR FOCI FORMATION AND IMMOBILIZATION OF THE AR .45
AA REDUCES AR CHROMATIN RECRUITMENT IN HUMAN PCA CELLS 50
AA INDUCES CELLULAR SENESCENCE IN HUMAN PCA TISSUE EX VIVO. 51
NOVEL ANTIANDROGENS INHIBIT AR TRANSCATIVATION .54
NOVEL AR ANTAGONISTS SLIGHTLY AFFECT AR NUCLEAR TRANSLOCATION 56
NOVEL AR ANTAGONISTS REDUCE AR N/C-TERMINAL INTERACTION 58
NOVEL AR ANTAGONISTS INFLUENCE INTRANUCLEAR DISTRIBUTION AND
MOBILITY OF THE AR 60
05 DISCUSSION 65
AR IN THE CYTOPLASM AND THE IMPACT OF NOVEL ANTAGONISTS ON NUCLEAR
TRANSLOCATION 66
AR CONFORMATIONAL CHANGE 70
AR WITHIN THE NUCLEUS 7.3
CELLULAR SENESCENCE AS TUMOR SUPPRESSIVE MECHANISM IN PCA 77
NOVEL AR ANTAGONISTS 78
CONCLUSION & OUTLOOK 81
06 REFERENCES 84
07 APPENDIX 103
SUPPLEMENTARY DATA 1.03
EHRENWORTLICHE ERKLARUNG. 1.05
CURRICULUM VITAE 106
DANKSAGUNG. 109
|
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| spelling | Heßenkemper, Wiebke Verfasser aut Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists von Wiebke Heßenkemper 2014 109 Bl. Ill., graph. Darst. 29,5 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2014 Wirkungsmechanismus (DE-588)4292866-7 gnd rswk-swf Inhibition (DE-588)4273127-6 gnd rswk-swf Androgen-Rezeptor (DE-588)4619850-7 gnd rswk-swf Antiandrogene (DE-588)4137601-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Androgen-Rezeptor (DE-588)4619850-7 s Inhibition (DE-588)4273127-6 s Antiandrogene (DE-588)4137601-8 s Wirkungsmechanismus (DE-588)4292866-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027302284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heßenkemper, Wiebke Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists Wirkungsmechanismus (DE-588)4292866-7 gnd Inhibition (DE-588)4273127-6 gnd Androgen-Rezeptor (DE-588)4619850-7 gnd Antiandrogene (DE-588)4137601-8 gnd |
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| title | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists |
| title_auth | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists |
| title_exact_search | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists |
| title_full | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists von Wiebke Heßenkemper |
| title_fullStr | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists von Wiebke Heßenkemper |
| title_full_unstemmed | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists von Wiebke Heßenkemper |
| title_short | Analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists |
| title_sort | analysis of the molecular mechanisms of androgen receptor inhibition by novel antagonists |
| topic | Wirkungsmechanismus (DE-588)4292866-7 gnd Inhibition (DE-588)4273127-6 gnd Androgen-Rezeptor (DE-588)4619850-7 gnd Antiandrogene (DE-588)4137601-8 gnd |
| topic_facet | Wirkungsmechanismus Inhibition Androgen-Rezeptor Antiandrogene Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027302284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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