Yeast protocols:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
New York [u.a.]
Humana Press
2014
|
Ausgabe: | 3. ed. |
Schriftenreihe: | Methods in molecular biology
1163 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | Originally published in 2006 |
Beschreibung: | X, 313 S. Ill., graph. Darst. |
ISBN: | 9781493907984 |
Internformat
MARC
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490 | 1 | |a Methods in molecular biology |v 1163 | |
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Datensatz im Suchindex
_version_ | 1804152157640851456 |
---|---|
adam_text | Titel: Yeast protocols
Autor: Xiao, Wei
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Basic Investigations in Saccharomyces cerevisiae........................ 1
Brendan P.G. Curran and Virginia Bugeja
2 Isolation of Yeast Nucleic Acids.................................... 15
Michael Biss, Michelle D. Hanna, and Wei Xiao
3 Quantification of GPP Signals by Fluorescent Microscopy and Flow Cytometry 23
Ting Wei and HepingDai
4 Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method......... 33
A. Daniel Gietz
5 Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
45
Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Marcus M. Langen,
and Ursula Fleig
6 See Catch Method for Studying Protein Complexes in Yeast Cells: A Technique
Unifying Fluorescence Microscopy and Mass Spectrometry............... 75
Changhui Deng and Andrew N. Krutchinsky
7 Yeast Two-Hybrid Liquid Screening................................ 97
Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz
8 Targeted Mutagenesis of a Specific Gene in Yeast...................... 109
Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz
9 Fluorescence Staining of Mitochondria for Morphology Analysis in Saccharomyces
cerevisiae..................................................... 131
Kai Stefan Dimmer
10 Assays for Autophagy I: The Cvt Pathway and Nonselective Autophagy...... 153
Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie
11 Assays for Autophagy II: Mitochondrial Autophagy..................... 165
Tomotake Kanki and Koji Okamoto
12 Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by
Fractionation, Protease Protection, and Carbonate Extraction............. 175
Danielle Hagstrom and Changle Ma
13 Yeast Survival and Growth Assays.................................. 183
Xin Xu, Amanda D. Lambrecht, and Wei Xiao
14 Spontaneous Mutagenesis Assay................................... 193
Susan Blackwell, Michelle D. Hanna, and Wei Xiao
15 Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene 201
Bernard A. Kunz
vii
viii Contents
16 pißßyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast
Schizosaccharomyces pombe........................................ 213
Jun Li and Li-Lin Du
17 Replicative and Chronological Life-Span Assays........................ 223
Spike D.L. Postnikoff and Troy A.A. Harkness
18 Detection of Protein Arginine Methylation in Saccharomyces cerevisiae....... 229
Christopher A. Jackson and Michael C. Tu
19 Detection of Protein Posttranslational Modifications from Whole-Cell Extracts
in Saccharomyces cerevisiae........................................ 249
Lindsay G. Ball and Wei Xiao
20 Ligand Engineering Using Yeast Surface Display....................... 257
Benjamin J. Hackel
21 Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid
Assay........................................................ 273
Kris Barreto and C. Ronald Geyer
Index............................................................... 311
|
any_adam_object | 1 |
author_GND | (DE-588)137120672 |
building | Verbundindex |
bvnumber | BV041825048 |
classification_rvk | WC 5700 |
classification_tum | BIO 260f BIO 325f |
ctrlnum | (OCoLC)891651769 (DE-599)BVBBV041825048 |
dewey-full | 579 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 579 - Microorganisms, fungi & algae |
dewey-raw | 579 |
dewey-search | 579 |
dewey-sort | 3579 |
dewey-tens | 570 - Biology |
discipline | Biologie |
edition | 3. ed. |
format | Book |
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id | DE-604.BV041825048 |
illustrated | Illustrated |
indexdate | 2024-07-10T01:06:15Z |
institution | BVB |
isbn | 9781493907984 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027270039 |
oclc_num | 891651769 |
open_access_boolean | |
owner | DE-11 DE-M49 DE-BY-TUM DE-703 |
owner_facet | DE-11 DE-M49 DE-BY-TUM DE-703 |
physical | X, 313 S. Ill., graph. Darst. |
publishDate | 2014 |
publishDateSearch | 2014 |
publishDateSort | 2014 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology |
spelling | Yeast protocols Ed. by Wei Xiao 3. ed. New York [u.a.] Humana Press 2014 X, 313 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1163 Originally published in 2006 Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Biotechnologie (DE-588)4069491-4 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 s Biotechnologie (DE-588)4069491-4 s Methode (DE-588)4038971-6 s DE-604 Xiao, Wei 1957- Sonstige (DE-588)137120672 oth Erscheint auch als Online-Ausgabe 978-1-4939-0799-1 Methods in molecular biology 1163 (DE-604)BV035362695 1163 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027270039&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Yeast protocols Methods in molecular biology Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Biotechnologie (DE-588)4069491-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Methode (DE-588)4038971-6 gnd |
subject_GND | (DE-588)4069491-4 (DE-588)4230509-3 (DE-588)4038971-6 |
title | Yeast protocols |
title_auth | Yeast protocols |
title_exact_search | Yeast protocols |
title_full | Yeast protocols Ed. by Wei Xiao |
title_fullStr | Yeast protocols Ed. by Wei Xiao |
title_full_unstemmed | Yeast protocols Ed. by Wei Xiao |
title_short | Yeast protocols |
title_sort | yeast protocols |
topic | Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Biotechnologie (DE-588)4069491-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Methode (DE-588)4038971-6 gnd |
topic_facet | Yeast fungi / Biotechnology / Laboratory manuals Yeast fungi / Laboratory manuals Biotechnologie Hefeartige Pilze Methode |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027270039&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT xiaowei yeastprotocols |