Polyadenylation: methods and protocols
In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2014
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| Schriftenreihe: | Methods in molecular biology
1125 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls |
| Beschreibung: | Bibliogr. en fin de chapitres. Index |
| Beschreibung: | XIV, 377 S. Ill. 26 cm |
| ISBN: | 9781627039703 |
Internformat
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| 520 | |a In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: Polyadenylation
Autor: Rorbach, Joanna
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... xi
Part I 3 -End Processing in Eukaryotes
1 3 End Formation and Regulation of Eukaryotic mRNAs................ 3
Thomas J. Sweet and Donny D. Lieatalosi
2 Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay.............. 13
Deepak P. Patil, Baskar Bakthavachalu, and Daniel R. Schoenberg
3 Using Klenow-Mediated Extension to Measure Poly (A)-Tail Length
and Position in the Transcriptome.................................. 25
Man Chun Lee, Amrei Jänicke, and Traude Helene Beilharz
4 Detection of Uridylated mRNAs................................... 43
François M. Sement and Dominique Gagliardi
5 Cytoplasmic Polyadenylation Assays................................ 53
Olga Coll, Ana Villalba, and Fdtima Gebauer
6 In Vitro Analysis of Transcriptional Activators and Polyadenylation......... 65
Takashi Nagaike and James L. Manley
7 Fluorescence Detection of Polyadenylation Reaction
Through the Coordination of Adenosine2-Coralyne-Adenosine2........... 75
Jia-Hui Lin and Wei-Lung Tseng
8 Small Molecule Recognition of Poly(A).............................. 81
özgül Persil Çetinkol
9 The Stem-Loop Luciferase Assay for Polyadenylation (SLAP)
Method for Determining CstF- 64- Dependent Polyadenylation Activity...... 109
J. Andrew Hockert and Clinton C. MacDonald
10 Accurate Mapping of Cleavage and Polyadenylation
Sites by 3 Region Extraction and Deep Sequencing.................... 119
Mainul Hoque, Wencheng Li, and Bin Tian
11 Polyadenylation Site Prediction Using PolyA-iEP Method................ 131
Ioannis Kavakiotis, George Tzanis, and Ioannis Vlahavas
12 Detecting Alternative Polyadenylation from Microarray Data.............. 141
Antonio Lembo and Paolo Provero
13 Quantitative Polyadenylation Site Mapping
with Single-Molecule Direct RNA Sequencing........................ 145
Fatih Ozsolak
vii
viii
Contents
14 RHAPA: A New Method to Quantify Alternative Polyadenylation.......... 157
Ashley L. Cornett and Carol S. Lutz
15 Multiplex Analysis of PolyA- Linked Sequences ( MAPS ) :
An RNA-Seq Strategy to Profile Poly(A+) RNA....................... 169
Tu Zhou, Hai-Ri Li, Jie Huang, Ge Jin, and Xiang-Dong Fu
16 Global and Quantitative Profiling of Polyadenylated RNAs
Using PAS-seq................................................ 179
Chengguo Yao and Tongsheng Shi
17 High-Throughput Sequencing of RNA Isolated
by Cross-Linking and Immunoprecipitation (HITS-CLIP)
to Determine Sites of Binding of CstF-64 on Nascent RNAs.............. 187
Petar N. Grozdanov and Clinton C. MacDonald
Part II Bacteria and Organelles
18 Polyadenylation in Bacteria and Organelles........................... 211
Joanna Rorbach, Agnieszka Bobrowicz, Sarah Pearce,
and Michal Minczuk
19 In Vivo Analysis of Polyadenylation in Prokaryotes..................... 229
Bijoy K. Mohanty and Sidney R. Kushner
20 Polyadenylation of RNA in E. coli: RNA
Polymerase-Associated (rA)n-Synthetic Activities....................... 251
Grant A. Encalade and Maxim V. Sukhodolets
21 Analysis of the Human Mitochondrial Transcriptome
Using Directional Deep Sequencing and Parallel Analysis of RNA Ends...... 263
Oliver Rackham and Aleksandra Filipovska
22 Measurement of Mitochondrial RNA Stability by Metabolic
Labeling of Transcripts with 4-Thiouridine........................... 277
Lukasz S. Borowski and Roman J. Szczesny
Part III Deadenylation and Decay
23 Deadenylation and Its Regulation in Eukaryotic Cells................... 289
Xiaokan Zhang, Frida E. Kleiman, and Emral Devany
24 Assaying mRNA Deadenylation In Vitro............................. 297
Mandy Jeske, Claudia Temme, and Elmar Wahle
25 Assaying mRNA Deadenylation In Vivo............................. 313
Claudia Temme and Elmar Wahle
26 In Vitro Analysis of RNA Degradation Catalyzed
by Deadenylase Enzymes......................................... 325
Joel Hrit, Nathan Raynard, Jamie Van Etten, Kamya Sankar,
Adam Petterson, and Aaron C. Goldstrohm
Contents ix
27 MicroRNA-Mediated Deadenylation in a Mammalian Cell-Free System............341
Motoaki Wakiyama and Shigeyuki Tokoyama
28 Quantitative Analysis of Deadenylation-Independent
mRNA Decay by a Modified MBRACE Assay..................................................353
Julie A. Dougherty, Roshan Mascarenhas, and Daniel R. Schoenberg
Erratum To............................................................................................................El
Erratum..................................................................................................................E3
Index..............................................................................................................................373
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Rorbach, Joanna Verfasser (DE-588)1059446987 aut Polyadenylation methods and protocols ed. by Joanna Rorbach and Agnieszka Bobrowicz New York [u.a.] Humana Press 2014 XIV, 377 S. Ill. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1125 Springer protocols Bibliogr. en fin de chapitres. Index In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls Cytologie ram ARN messagers ram Biologie cellulaire ARN messager Gene expression Mammals / Genetics Erscheint auch als Online-Ausgabe 978-1-62703-971-0 Methods in molecular biology 1125 (DE-604)BV035362695 1125 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027259219&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rorbach, Joanna Polyadenylation methods and protocols Methods in molecular biology Cytologie ram ARN messagers ram Biologie cellulaire ARN messager Gene expression Mammals / Genetics |
| title | Polyadenylation methods and protocols |
| title_auth | Polyadenylation methods and protocols |
| title_exact_search | Polyadenylation methods and protocols |
| title_full | Polyadenylation methods and protocols ed. by Joanna Rorbach and Agnieszka Bobrowicz |
| title_fullStr | Polyadenylation methods and protocols ed. by Joanna Rorbach and Agnieszka Bobrowicz |
| title_full_unstemmed | Polyadenylation methods and protocols ed. by Joanna Rorbach and Agnieszka Bobrowicz |
| title_short | Polyadenylation |
| title_sort | polyadenylation methods and protocols |
| title_sub | methods and protocols |
| topic | Cytologie ram ARN messagers ram Biologie cellulaire ARN messager Gene expression Mammals / Genetics |
| topic_facet | Cytologie ARN messagers Biologie cellulaire ARN messager Gene expression Mammals / Genetics |
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