Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2013 [erschienen] 2014
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo |
| Beschreibung: | 103 S. Ill., graph. Darst. |
| ISBN: | 9783863874650 |
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| 245 | 1 | 0 | |a Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |c submitted by Zhenya Wang |
| 246 | 1 | 3 | |a Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2013 [erschienen] 2014 | |
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| 500 | |a Nebentitel: Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo | ||
| 502 | |a Berlin, Freie Univ., Diss., 2014 | ||
| 650 | 7 | |a influenza |2 cabt | |
| 650 | 7 | |a swine influenza virus |2 cabt | |
| 650 | 7 | |a probiotics |2 cabt | |
| 650 | 7 | |a Enterococcus faecium |2 cabt | |
| 650 | 7 | |a zinc |2 cabt | |
| 650 | 7 | |a immune response |2 cabt | |
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| 650 | 0 | 7 | |a Virusinfektion |0 (DE-588)4188401-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Schweineinfluenzavirus |0 (DE-588)4235667-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Probiotikum |0 (DE-588)4338112-1 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
LIST OF ABBREVIATIONS 4
1 INTRODUCTION 7
1. 1 SWINE INFLUENZA VIRUS 7
1. 1. 1 HISTORICAL OVERVIEW 7
1.1.2 CLINICAL
DISEASE
IN PIGS 8
1.1.3 STRUCTURE AND
CLASSIFICATION 9
1. 1. 4 VIRUS TRANSMISSION 10
1.1.5 PREVENTION
AND
TREATMENT 11
1.1.6 IMMUNE
RESPONSE 12
1. 2 PROBIOTICS 14
1. 2. 1 OVERVIEW OF PROBIOTICS 14
1.2.2 ENTEROCOCCUS (E.) FAECIUM AS A PROBIOTIC 15
1. 2. 3 PROBIOTICS IN VIRUS INFECTIONS 15
1. 2. 4 MECHANISMS OF PROBIOTICS IN VIRUS INFECTIONS 16
1. 2. 5 PROBIOTICS AND INFLUENZA VIRUS INFECTION 17
1. 3 ZINC 19
1. 3. 1 OVERVIEW OF ZINC 19
1. 3. 2 ZINC OXIDE 20
1. 3. 3 ZINC IN VIRUS INFECTIONS 20
1. 3. 4 ROLE OF ZINC IN VIRUS INFECTIONS 21
1. 3. 5 ZINC AND INFLUENZA VIRUS INFECTION 22
1. 4 AIM OF STUDY 23
2 MATERIALS AND METHODS 25
2.1 IN VITRO STUDIES 25
2. 1. 1 VIRUS 25
2. 1. 2 CELL LINES 25
2. 1. 3 PROBIOTIC E. FAECIUM 25
2. 1. 4 EXPERIMENTAL DESIGN OF INTERFERENCE EXPERIMENTS 26
1
HTTP://D-NB.INFO/1050304918
TABLE OF CONTENTS
2. 1. 5 CELL CULTURE 27
2.1.6 VIRUS
PROPAGATION 28
2. 1. 7 MTT ASSAY 28
2. 1. 8 TCID
5
O ASSAY 28
2. 1. 9 ASSESSMENT OF NITRIC OXIDE (NO) RELEASE 29
2. 1. 10RNA EXTRACTION 29
2. 1. 11 CDNA SYNTHESIS 30
2. 1. 12 REAL-TIME PCR TO ASSESS THE EXPRESSION OF IMMUNE MEDIATORS 30
2. 1. 13 VIRUS ADSORPTION TO E. FAECIUM (PREINCUBATION ASSAY) 32
2. 1. 14 ELECTRON MICROSCOPY 33
2. 1. 15 STATISTICAL ANALYSIS 33
2. 2 IN VIVO STUDIES 34
2. 2. 1 VIRUS 34
2. 2. 2 VACCINE 34
2. 2. 3 FEED ADDITIVES 34
2. 2. 4 ANIMALS AND EXPERIMENTAL SETUP 34
2. 2. 5 CLINICAL FOLLOW-UP AND SAMPLING 36
2. 2. 6 PATHOLOGY 37
2. 2. 7 ANTIBODY ELISA 37
2. 2. 8 HEMAGGLUTINATION INHIBITION (HI) ASSAY 38
2. 2. 9 VIRAL RNA QUANTIFICATION FROM SWABS 38
2. 2. 10 DIFFERENTIAL CELL COUNT 38
2. 2. 11 FLOW CYTOMETRY 39
2. 2. 12 STATISTICAL ANALYSIS 40
3 RESULTS 41
3. 1 IN VITRO STUDIES 41
3. 1. 1 CELLS 41
3. 1. 2 VIRUS PROPAGATION 41
3. 1. 3 CYTOTOXICITY EFFECT OF E. FAECIUM ON 3D4/21 AND MDBK CELLS 42
3. 1. 4 EFFECT OF E. FAECIUM ON SIV INFECTED CELLS AS DETECTED BY MTT
ASSAY 44
2
TABLE OF CONTENTS
3. 1. 5 VIRUS TITER REDUCTIONS IN CELLS TREATED WITH E. FAECIUM 45
3. 1. 6 E. FAECIUM INCREASES THE PRODUCTION OF NO 46
3. 1. 7 VIRUS ADSORPTION BY E. FAECIUM 47
3.1.8 CYTOKINE
EXPRESSION UNDER
THE
INFLUENCE
OF E. FAECIUM 48
3. 2 IN VIVO STUDIES 50
3. 2. 1 CLINICAL SYMPTOMS AND WEIGHT GAINS 50
3. 2. 2 GROSS PATHOLOGY 52
3. 3. 3 HISTOPATHOLOGY AND LESION SCORE BY HE STAINING 53
3. 2. 4 VIROLOGICAL ANALYSIS 54
3. 2. 5 ANTIBODY ELISA 55
3. 2. 6 HI ASSAY 56
3. 2. 7 DIFFERENTIAL CELL COUNT 57
3. 2. 8 CELLULAR IMMUNE RESPONSES 58
4 DISCUSSION 63
4. 1 IN VITRO STUDY 63
4. 2 IN VIVO STUDY 68
5 SUMMARY 73
6 ZUSAMMENFASSUNG 75
7 REFERENCES 79
PUBLICATIONS 99
ACKNOWLEDGMENTS 101
3
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| spelling | Wang, Zhenya Verfasser aut Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo submitted by Zhenya Wang Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo Berlin Mensch und Buch Verl. 2013 [erschienen] 2014 103 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo Berlin, Freie Univ., Diss., 2014 influenza cabt swine influenza virus cabt probiotics cabt Enterococcus faecium cabt zinc cabt immune response cabt Schweinekrankheit (DE-588)4132374-9 gnd rswk-swf Futterzusatz (DE-588)4155774-8 gnd rswk-swf Virusinfektion (DE-588)4188401-2 gnd rswk-swf Schweineinfluenzavirus (DE-588)4235667-2 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Zinkoxid (DE-588)4190864-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Schweinekrankheit (DE-588)4132374-9 s Virusinfektion (DE-588)4188401-2 s Schweineinfluenzavirus (DE-588)4235667-2 s Futterzusatz (DE-588)4155774-8 s Probiotikum (DE-588)4338112-1 s Zinkoxid (DE-588)4190864-8 s DE-604 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000096538 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027258706&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Zhenya Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo influenza cabt swine influenza virus cabt probiotics cabt Enterococcus faecium cabt zinc cabt immune response cabt Schweinekrankheit (DE-588)4132374-9 gnd Futterzusatz (DE-588)4155774-8 gnd Virusinfektion (DE-588)4188401-2 gnd Schweineinfluenzavirus (DE-588)4235667-2 gnd Probiotikum (DE-588)4338112-1 gnd Zinkoxid (DE-588)4190864-8 gnd |
| subject_GND | (DE-588)4132374-9 (DE-588)4155774-8 (DE-588)4188401-2 (DE-588)4235667-2 (DE-588)4338112-1 (DE-588)4190864-8 (DE-588)4113937-9 |
| title | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |
| title_alt | Wirkung von Nahrungs-Enterococcus faecium NCIMB 10415 und Zinkoxid auf Schweine-Influenza-Virus-Infektion in vitro und in vivo |
| title_auth | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |
| title_exact_search | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |
| title_full | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo submitted by Zhenya Wang |
| title_fullStr | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo submitted by Zhenya Wang |
| title_full_unstemmed | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo submitted by Zhenya Wang |
| title_short | Effect of dietary Enterococcus faecium NCIMB 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |
| title_sort | effect of dietary enterococcus faecium ncimb 10415 and zinc oxide on porcine influenza virus infection in vitro and in vivo |
| topic | influenza cabt swine influenza virus cabt probiotics cabt Enterococcus faecium cabt zinc cabt immune response cabt Schweinekrankheit (DE-588)4132374-9 gnd Futterzusatz (DE-588)4155774-8 gnd Virusinfektion (DE-588)4188401-2 gnd Schweineinfluenzavirus (DE-588)4235667-2 gnd Probiotikum (DE-588)4338112-1 gnd Zinkoxid (DE-588)4190864-8 gnd |
| topic_facet | influenza swine influenza virus probiotics Enterococcus faecium zinc immune response Schweinekrankheit Futterzusatz Virusinfektion Schweineinfluenzavirus Probiotikum Zinkoxid Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000096538 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027258706&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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