Influence of membrane lipids on T cell antigen receptor function:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Enth. außerdem 5 Sonderabdr. aus verschiedenen Zeitschr. |
| Beschreibung: | 115 S. Ill., graph. Darst. 30 cm |
Internformat
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| 245 | 1 | 0 | |a Influence of membrane lipids on T cell antigen receptor function |c eingereicht von Katharina Beck |
| 264 | 1 | |c 2013 | |
| 300 | |a 115 S. |b Ill., graph. Darst. |c 30 cm | ||
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Datensatz im Suchindex
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| adam_text | INDEX
I ABSTRACT 6
II INTRODUCTION 7
2.1. T CELLS 7
2.2. THETCR 7
2.2.1.TCRSTOICHIOMETR
Y 7
2.2.2.TCR NANOCLUSTERING 8
2.3. INTERACTION OF
THE TCR WITH LIPIDS 9
2.3.1.DEFINITION
S 9
2.3.2.ROLE OF PHOSPHOLIPID BINDING
TO THE TCR 10
2.4. TCR ACTIVATION MODELS 10
2.4.1.THE PERMISSIVE GEOMETRY MODEL 11
2.4.2.KLNETIC SEGREGATION MODEL 12
2.4.3.THE LIPID BASED
MODEL 12
2.5. TCR SIGNALLING 14
2.6. LIPID RAFTS 16
2.6.1.THE TCR
AND LIPID RAFTS 17
2.7. LIPID-PROTEIN STUDIES 18
III OBJECTIVES 19
IV RESULTS 20
4.1. CHOLESTEROL BINDING TO
TCRP
GUARDS THE RESTING
STATE OF THE
T
CELL RECEPTOR ....20
4.2. THE T CELL RECEPTOR BINDS TO UNSATURATED
FATTY ACIDS AND
LOCALIZES TO
THE NON-
RAFT DOMAIN 56
4.3. CHOLESTEROL AND
SPHINGOMYELIN DRIVE LIGAND-INDEPENDENT T
CELL ANTIGEN RECEPTOR
NANOCLUSTERING 76
V DISCUSSION 77
5.1. THE ROLE OF CHOLESTEROL IN TCR FUNCTION 77
5.1.1.A LIPID PULL DOWN
ASSAY TO STUDY THE INTERACTION BETWEEN PROTEINS AND LIPIDS
77
5.1.2
.CHOLESTEROL-BINDING TO TCRP
IS
LOST UPON THE CD3CC
78
5.1.3.CHOLESTEROL AND
SPHINGOMYELIN MIGHT BE DISPLACED TOGETHER FROM
THE TCR 79
5.1.4.MECHANISM BY WHICH CHOLESTEROL IS DISPLACED
FROM THE TCR 80
5.2.
TCR
ACTIVATION MODELS 82
5.2.1.THE PERMISSIVE GEOMETRY-KINETIC SEGREGATION
MODEL (CD3CC-KS) 82
VI MATERIALS AND METHODS 84
6.1. MOLECULAR BIOLOGY. 84
6.1.1.KIT
S 84
6.1.2.DNA SEPARATION VIA AGAROSE GELS 84
6.1.3.POLYMERASE CHAIN REACTION (PCR) 84
6.1.4.CLONING PROCEDURE 84
6.1.4.1.ENZYMATIC DIGEST 84
6.1.4.2.
LIGATION 85
6. L .43.
TRANSFORMATION 85
HTTP://D-NB.INFO/1046874837
INDEX
6.1.4.4.COLONY SCREEN 85
6.1.4.5.DETERMINATION OF DNA
CONCENTRATION 85
6.1.4.6.DNA SEQUENCING 86
6.1.5.SPECIFIC CLONING PROCEDURES 87
6.1.6.PRIMER LIST 92
6.1.7.GENERATED CONSTRUCT
LIST 94
6.1.8.PERFORMED PCRS 95
6.2. CELL BIOLOGICAL METHODS 96
6.2.1
.EXPERIMENTAL ANIMALS 96
6.2.2.PREPARATION OF
MOUSE PERIPHERAL BLOOD LYMPHOCYTES
(PBLS) FROM AN ORGAN 96
6.2.3ISOLATION OF PERIPHERAL BLOOD MONONUCLEAR
CELLS (PBMCS) 96
6.2.4.CELL LINES 96
6.2.5.FLOW CYTOMETRY 98
6.2.6.TRANSFECTION OF
PHOENIX CELLS 98
6.2.7.RETROVIRAL TRANSDUCTION OF
CELLS 98
6.2.8.TRANSFECTION OF
JURKAT- AND
2B4-BASED CELL
LINES 99
6.2.9.CELL STIMULATION 99
6.2.10.MEASUREMENT OFCA
2+
FLUX 99
6.2.11.CHOLESTEROL OXIDATION AND EXTRACTION 100
6.2.12.GIANT PLASMA MEMBRANE VESICULATION 100
6.3. PROTEIN BIOCHEMISTRY 100
6.3.1.ANTIBODIES 100
6.3.2.SH3-PD ASSAY,
LIPID-PD ASSAY, IP
AND WB 103
6.3.3.RECONSTITUTION OF
CD3
PHOSPHORYLATION 104
6.3.4.MEMBRANE PREPARATIONS 104
6.3.5.PROTEIN RECONSTITUTION IN LIPOSOMES 104
6.4. BLUE NATIVE PAGE 105
6.4.1.DETECTION OF
PHOSPHORYLATED
T AND B CELL ANTIGEN RECEPTOR
SPECIES BY PHOS-TAG
SDS- AND
BLUE NATIVE-PAGE 105
6.5. CHEMICAL METHODS 106
6.5.1.
ANALYSIS OF
INTERACTIONS BETWEEN PROTEINS AND
FATTY ACIDS
OR CHOLESTEROL USING A
FATTY ACID/CHOLESTEROL PULL-DOWN ASSAY 106
6.5.2.PREPARATION OF LIPOSOMES 107
6.5.3.SYNTHESIS OF
NITROPHENYLSEPHAROSE 107
7. VII REFERENCES 108
8. VIII APPENDIX 115
8.1. FULL LIST OF PUBLICATIONS 115
8.2. MANUSCRIPTS IN PREPARATION 115
8.3. ACKNOWLEDGEMENTS 116
|
| any_adam_object | 1 |
| author | Beck, Katharina |
| author_GND | (DE-588)1046730436 |
| author_facet | Beck, Katharina |
| author_role | aut |
| author_sort | Beck, Katharina |
| author_variant | k b kb |
| building | Verbundindex |
| bvnumber | BV041802981 |
| ctrlnum | (OCoLC)879546420 (DE-599)DNB1046874837 |
| dewey-full | 571.966 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.966 |
| dewey-search | 571.966 |
| dewey-sort | 3571.966 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T01:05:43Z |
| institution | BVB |
| language | English |
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| physical | 115 S. Ill., graph. Darst. 30 cm |
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| spelling | Beck, Katharina Verfasser (DE-588)1046730436 aut Influence of membrane lipids on T cell antigen receptor function eingereicht von Katharina Beck 2013 115 S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Enth. außerdem 5 Sonderabdr. aus verschiedenen Zeitschr. Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027248478&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Beck, Katharina Influence of membrane lipids on T cell antigen receptor function |
| subject_GND | (DE-588)4113937-9 |
| title | Influence of membrane lipids on T cell antigen receptor function |
| title_auth | Influence of membrane lipids on T cell antigen receptor function |
| title_exact_search | Influence of membrane lipids on T cell antigen receptor function |
| title_full | Influence of membrane lipids on T cell antigen receptor function eingereicht von Katharina Beck |
| title_fullStr | Influence of membrane lipids on T cell antigen receptor function eingereicht von Katharina Beck |
| title_full_unstemmed | Influence of membrane lipids on T cell antigen receptor function eingereicht von Katharina Beck |
| title_short | Influence of membrane lipids on T cell antigen receptor function |
| title_sort | influence of membrane lipids on t cell antigen receptor function |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027248478&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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