Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache. - Online erschienen: 2014 |
| Beschreibung: | X, 117 Bl. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication |c von Jennifer Reinboth |
| 246 | 1 | 3 | |a Beteiligung zellulärer Faktoren an der Permissivität von Wirtszellen in Unterstützung der onkolytischen Vaccinia Virus Replikation |
| 264 | 1 | |c 2012 | |
| 300 | |a X, 117 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache. - Online erschienen: 2014 | ||
| 502 | |a Würzburg, Univ., Diss., 2012 | ||
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
SUMMARY . IV
ZUSAMMENFASSUNG VII
1 INTRODUCTION 1
1.1 CANCER - STATISTICS AND BIOLOGY 1
1.1.1 MELANOMA 2
1.1.1.1 MELANOMA GENETICS - AN OVERVIEW 3
1.1.1.2 CELL LINES 888-MEL AND 1936-MEL 5
1.1.2 CANCER MANAGEMENT 6
1.1.2.1 ONCOLYTIC VIROTHERAPY 7
1.2 VACCINIA VIRUS 8
1.2.1 POXVIRIDAE - TAXONOMY 8
1.2.2 HISTORY OF POXVIRIDAE AND THEIR APPLICATION IN ONCOLYTIC
VIROTHERAPY 8
1.2.3 MORPHOLOGY OF VACCINIA VIRUSES 11
1.2.4 VACCINIA VIRUS LIFE CYCLE 12
1.2.4.1 VACCINIA VIRUS CELL ENTRY 12
1.2.4.2 VACCINIA VIRUS REPLICATION 12
1.2.4.3 VACCINIA VIRUS GENE EXPRESSION 14
1.2.5 VACCINIA VIRUS-HOST INTERACTOME 18
1.2.5.1 IMMUNE EVASION 18
1.2.5.2 HOST FACTORS IN VACCINIA VIRUS LIFE CYCLE (NOT IMMEDIATELY
IMMUNE-RELATED) 20
1.3 AIM OF THE STUDY 22
2 MATERIAL AND METHODS 24
2.1 MATERIAL 24
2.1.1 DEVICES 24
2.1.2 REAGENTS 25
2.1.3 ITEMS 26
2.1.4 KITS 27
2.1.5 SYNTHETIC OLIGONUCLEOTIDES (PRIMER) 27
2.1.6 SOFTWARE 28
2.1.6 COMPOSITION OF BUFFERS AND MEDIA 28
2.1.7 CELL LINES 30
2.1.8 VACCINIA VIRUS ISOLATES 31
2.1.8.1 RECOMBINANT VACCINIA VIRUSES 31
2.1.8.2 WILD-TYPE VACCINIA VIRUS ISOLATES 32
HTTP://D-NB.INFO/1050009932
2.2 METHODS 33
2.2.1 CELL CULTURE 33
2.2.1.1 CULTURING OF ADHERENT HUMAN CANCER CELLS 33
2.2.1.2 CELL PASSAGING . . 33
2.2.1.3 CELL COUNTING 33
2.2.1.4 CONFLUENCE TEST 34
2.2.1.5 FREEZING AND THAWING CELLS 34
2.2.2 VIROLOGICAL METHODS 35
2.2.2.1 VACV INFECTION OF HUMAN CANCER CELLS 35
2.2.2.2 VACCINIA VIRUS TITRATION VIA PLAQUE ASSAY 36
2.2.2.3 VIRAL REPLICATION ASSAY 36
2.2.2.4 INFECTION OF HUMAN CANCER CELLS FOR FLOW CYTOMETRY 36
2.2.3 MOLECULAR BIOLOGICAL METHODS 37
2.2.3.1 MICROARRAY 37
2.2.3.2 MICRORNA ARRAY 41
2.2.3.3 ARRAY COMPARATIVE GENOMIC HYBRIDIZATION (CGH) 42
2.2.3.4 FLOW CYTOMETRY 42
2.2.4 STATISTICAL METHODS 43
3 RESULTS 45
3.1 CORRELATES BETWEEN HOST AND VIRAL TRANSCRIPTIONAL PROGRAM ASSOCIATED
WITH DIFFERENT
ONCOLYTIC VACCINIA VIRUS ISOLATES 45
3.1.1 VACV-DEPENDENT CHANGES IN HOST CELL TRANSCRIPTION OVER TIME 45
3.1.1.1 VACV-CAUSED EFFECTS ON HUMAN GENE EXPRESSION OVER TIME 45
3.1.1.2 INFECTED AND UNINFECTED SAMPLES SEGREGATE TOGETHER ACCORDING TO
TIME IN CULTURE ....47
3.1.1.2.1 A MULTISTEP FILTER REVEALS TRANSCRIPTIONAL CHANGES SPECIFIC TO
VACV INFECTION 48
3.1.1.3 VACV EFFECTS ON HUMAN GENE TRANSCRIPTION 49
3.1.2 VACCINIA VIRUS - DEPENDENT CHANGES IN VIRAL GENE TRANSCRIPTION 50
3.1.3 VACCINIA VIRUS ISOLATE-SPECIFIC CHANGES IN TRANSCRIPTION AND
CORRELATION WITH VIRAL
REPLICATION 53
3.1.3.1 VIRAL REPLICATION ANALYSIS 53
3.1.3.2 RANKING OF VACV GENE TRANSCRIPTION LEVEL AND CORRELATION WITH
REPLICATION 56
3.1.4 CORRELATES BETWEEN VACV AND HOST CELL TRANSCRIPTION 59
3.1.4.1 CORRELATION BETWEEN VIRAL AND HUMAN EARLY GENE TRANSCRIPTION 59
3.1.5 PREDICTION OF VACV REPLICATION 61
3.2 CONTRIBUTING FACTORS TO PERMISSIVENESS OF MELANOMA CELL LINES TO
ONCOLYTIC VIROTHERAPY ....64
3.2.1 EVALUATION OF GENERAL CONDITIONS FOR THE 15-MEL STUDY 64
3.2.1.1 APPLICABILITY OF VIRUS-EXPRESSED FLUOROCHROMES FOR FLOW
CYTOMETRIC EVALUATION
OF VIRAL REPLICATION 64
3.2.1.2 MOI-DEPENDENT INFECTION CHARACTERISTICS 66
3.2.2 PERMISSIVENESS OF THE 15-MEL PANEL TO GLV-LH68 INFECTION 67
3.2.3 MOLECULAR BIOLOGICAL VARIANCES ASSOCIATED WITH PERMISSIVENESS TO
VACV TREATMENT....69
3.2.3.1 TRANSCRIPTIONAL PATTERNS RELATED TO A VACV PERMISSIVE PHENOTYPE
69
3.2.3.2 MICRORNA EXPRESSIONAL VARIANCES ASSOCIATED WITH PERMISSIVENESS
TO VACV
TREATMENT AND CORRELATION WITH MRNA TRANSCRIPTION 71
3.2.3.3 COPY NUMBER VARIATIONS ASSOCIATED WITH PERMISSIVENESS :. 74
4 DISCUSSION 78
5 REFERENCES 86
6 APPENDICES 99
6.1 ADDITIONAL TABLES 99
6.2 LIST OF FIGURES 104
6.3 LIST OF TABLES 105
6.4 LIST OF ABBREVIATIONS 106
6.5 EIDESSTATTLICHE ERLDARUNG ILL
6.6 CURRICULUM VITAE 112
6.7 PUBLICATION LIST 114
6.8 CONFERENCES 115
ACKNOWLEDGMENTS 116
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| spelling | Reinboth, Jennifer 1983- Verfasser (DE-588)104991841X aut Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication von Jennifer Reinboth Beteiligung zellulärer Faktoren an der Permissivität von Wirtszellen in Unterstützung der onkolytischen Vaccinia Virus Replikation 2012 X, 117 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache. - Online erschienen: 2014 Würzburg, Univ., Diss., 2012 Onkolyse (DE-588)4172588-8 gnd rswk-swf Melanom (DE-588)4074707-4 gnd rswk-swf Microarray (DE-588)4544227-7 gnd rswk-swf Vaccinia-Virus (DE-588)4187306-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Vaccinia-Virus (DE-588)4187306-3 s Melanom (DE-588)4074707-4 s Microarray (DE-588)4544227-7 s Onkolyse (DE-588)4172588-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-85392 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-85392 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027241209&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Reinboth, Jennifer 1983- Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication Onkolyse (DE-588)4172588-8 gnd Melanom (DE-588)4074707-4 gnd Microarray (DE-588)4544227-7 gnd Vaccinia-Virus (DE-588)4187306-3 gnd |
| subject_GND | (DE-588)4172588-8 (DE-588)4074707-4 (DE-588)4544227-7 (DE-588)4187306-3 (DE-588)4113937-9 |
| title | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication |
| title_alt | Beteiligung zellulärer Faktoren an der Permissivität von Wirtszellen in Unterstützung der onkolytischen Vaccinia Virus Replikation |
| title_auth | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication |
| title_exact_search | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication |
| title_full | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication von Jennifer Reinboth |
| title_fullStr | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication von Jennifer Reinboth |
| title_full_unstemmed | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication von Jennifer Reinboth |
| title_short | Cellular Factors Contributing to Host Cell Permissiveness in Support of Oncolytic Vaccinia Virus Replication |
| title_sort | cellular factors contributing to host cell permissiveness in support of oncolytic vaccinia virus replication |
| topic | Onkolyse (DE-588)4172588-8 gnd Melanom (DE-588)4074707-4 gnd Microarray (DE-588)4544227-7 gnd Vaccinia-Virus (DE-588)4187306-3 gnd |
| topic_facet | Onkolyse Melanom Microarray Vaccinia-Virus Hochschulschrift |
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