Verfügbarkeit: Shotgun proteomics

Shotgun proteomics: methods and protocols

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Bibliographische Detailangaben
Weitere Verfasser:	Martins-de-Souza, Daniel (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	New York Humana Press 2014
Schriftenreihe:	Methods in molecular biology 1156
Schlagworte:	Isotopenmarkierung Methode Proteomanalyse Massenspektrometrie Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIV, 492 S. Ill., graph. Darst.
ISBN:	9781493906840 1493906844

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MARC


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Datensatz im Suchindex

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adam_text	Titel: Shotgun proteomics Autor: Martins-de-Souza, Daniel Jahr: 2014 Contents Preface......................................................... v Contributors.................................................... xi Part I Introduction 1 Survey of Shotgun Proteomics................................... 3 Fabio Cesar Sorna Nogueira and Gilberto B. Domont Part II Sample Pre-fractionation 2 LC-MALDI-TOF/TOF for Shotgun Proteomics..................... 27 Patricia Fernández-Puente, Jesús Mateos, Francisco J. Blanco, and Cristina Ruiz-Romero 3 Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry.............. 39 Maggie P.T. Lam, Chun Hin Law, Quan Quan, Tun Zhao, and Ivan K. Chu 4 GeLC-MS/MS Analysis of Complex Protein Mixtures................. 53 Monika Dzieciatkowska, Ryan Hill, and Kirk C. Hansen 5 IPG Strip-Based Peptide Fractionation for Shotgun Proteomics........... 67 MuratEravci, Christian Sommer, and Matthias Selbach Part III In Vivo Stable Isotope Labeling 6 SILAC Yeast: From Labeling to Comprehensive Proteome Quantification........................................ 81 Lyris M.F. de Godoy 7 Analysis of Proteome Dynamics in Mice by Isotopic Labeling............ Ill John C. Price and Sina Ghaemmaghami 8 Stable Isotope Labeling in Mammals (SILAM)....................... 133 Daniel B. McClatchy and John R. Tates III 9 Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale...................................... 147 Stefan Reckow and Christian Webhofer Part IV In Vitro Stable Isotope Labeling 10 Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification................................. 157 Andy Christoforou, Alfonso Martinez Arias, and Kathryn S. Lilley vii viii Contents 11 Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)........................ 175 Giuseppina Maccarrone, Maria Lebar, and Daniel Martins-de-Souza 12 Employing TMT Quantification in a Shotgun-MS Platform............. 187 Darragh P. O Brien and John F. Timms 13 Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle...................... 201 Bruno Menezes de Oliveira Part V Label-Free Proteomics 14 Spectral Counting Label-Free Proteomics........................... 213 Liisa Arike and Lauri Peil 15 Quantification of Proteins by Label-Free LC-MSE..................... 223 Alon Savidor and Tishai Levin Part VI Proteomics Informatics and Quantitation 16 Bioinformatics for Proteomics: Opportunities at the Interface Between the Scientists, Their Experiments, and the Community........... 239 Marc Vaudel, Harald Barsnes, Lennart Martens, and Frode S. Berven Part VII Protein-Protein Interactions 17 Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry............................................ 251 Francisco M. Bastos de Oliveira and Marcus B. Smolka 18 Application of Shotgun Proteomics for Discovery-Driven Protein-Protein Interaction...................................... 265 Livia Goto-Silva, Zoltan Maliga, Mikolaj Slabicki, Jimmy Rodriguez Murillo, and Magno Junqueira 19 Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags......................................... 279 Antje Dittmann, Sonja Ghidelli-Disse, Carsten Hopf, and Marcus Bantscheff Part VIII Targeted Proteomics 20 Biomarker Verification Using Selected Reaction Monitoring and Shotgun Proteomics........................................ 295 Angel Mauricio Castro-Gamero, Clarice Izumi, and José César Rasa 21 Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers....... 307 NingQingLiu, Lennard J.M. Dekker, Martijn M. Van Duijn, and Arzu Umar Contents ¡x 22 The Secretóme Analysis by High-Throughput Proteomics and Multiple Reaction Monitoring (MRM).......................... 323 Vitor M. Faça, Camila S. Palma, Daniele Albuquerque, Gabriela N.S. Canchaya, Mariana L. Grassi, Vera L. EpifAnio, and Eduardo B. de Oliveira 23 Preparation of Heteroelement-Incorporated and Stable Isotope-Labeled Protein Standards for Quantitative Proteomics.......... 337 Anna Konopka, Nico Zinn, Christina Wild, and WolfD. Lehmann Part IX Posttranslational Modifications 24 One-Source Peptide/Phosphopeptide Ratio Standards for Accurate and Site-Specific Determination of the Degree of Phosphorylation..................................... Martin E. Boehm, Bettina Hahn, and WolfD. Lehmann 25 Quantitative Glycoproteomics for N-Glycoproteome Profiling .... Sheng Pan 26 A Practical Recipe to Survey Phosphoproteomes............... William C. Edelman, Kelsey M. Haas, Joanne I. Hsu, Robert T. Lawrence, and Judit Villén 27 Quantitation of the Phosphoproteome Using the Library-Assisted extracted Ion Chromatogram (LAXIC) Strategy............... Justine V. Arrington, Liang Xue, and W. Andy Too 28 Fast, Efficient, and Quality-Controlled Phosphopeptide Enrichment from Minute Sample Amounts Using Titanium Dioxide......... Clarissa Dickhut, Sonja Radau, and René P. Zahedi 29 Quantifying Small Molecule-Induced Changes in Cellular Protein Expression and Posttranslational Modifications Using Isobaric Mass Tags................................ Isabelle Becher, Maria Fdlth Savitski, and Marcus Bantscheff Part X Mass Spectrometry for Protein Analysis 30 Analysis of Protein Structure by Cross-Linking Combined with Mass Spectrometry........................................ 447 Evgeniy V. Petrotchenko, Karl AT. Makepeace, Jason J. Serpa, and Christoph H. Borchers 31 Top-Down Proteomics by Means of Orbitrap Mass Spectrometry......... 465 Kai Schejfler Index.......................................................... 489 367 379 389 407 417 431
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spellingShingle	Shotgun proteomics methods and protocols Methods in molecular biology Isotopenmarkierung (DE-588)4176811-5 gnd Methode (DE-588)4038971-6 gnd Proteomanalyse (DE-588)4596545-6 gnd Massenspektrometrie (DE-588)4037882-2 gnd
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title	Shotgun proteomics methods and protocols
title_auth	Shotgun proteomics methods and protocols
title_exact_search	Shotgun proteomics methods and protocols
title_full	Shotgun proteomics methods and protocols ed. by Daniel Martins-de-Souza
title_fullStr	Shotgun proteomics methods and protocols ed. by Daniel Martins-de-Souza
title_full_unstemmed	Shotgun proteomics methods and protocols ed. by Daniel Martins-de-Souza
title_short	Shotgun proteomics
title_sort	shotgun proteomics methods and protocols
title_sub	methods and protocols
topic	Isotopenmarkierung (DE-588)4176811-5 gnd Methode (DE-588)4038971-6 gnd Proteomanalyse (DE-588)4596545-6 gnd Massenspektrometrie (DE-588)4037882-2 gnd
topic_facet	Isotopenmarkierung Methode Proteomanalyse Massenspektrometrie Aufsatzsammlung
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