The role of slc7a7 in promoting brain colonization by microglia:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 80 Bl. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
ZUSAMMENF
ASSU
NG
ABSTRACT
ACKNOWLEDGEMENTS
1 INTRODUCTION 1
1.1 THE IMMUNE SYSTEM 1
1.2 THE IMMUNE SYSTEM IS OF HAEMATOPOIETIC ORIGIN 2
1.2.1 MICROGLIA, THE MACROPHAGES
OF THE BRAIN 4
1.2.2 MICROGLIA
ACTIVITY IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS 4
1.2.3 ADULT MICROGLIA
COME FROM PRIMITIVE MACROPHAGES 6
1.2.4 MONOCYTES
CAN BE AN EFFECTIVE MEANS
OF THERAPY DELIVERY TO THE CNS 8
1.2.5 DECREASE IN MICROGLIA NUMBERS IS LINKED TO NEUROLOGICAL DISORDERS
9
1.2.6 HAEMOCYTES ARE
RESIDENT PHAGOCYTES IN DROSOPHILA 10
1.3 ZEBRAFISH AS A MODEL FOR MICROGLIAL STUDIES 11
1.4 METABOLISM AND THE IMMUNE SYSTEM 12
1.4.1 THE L-TYPE AND
Y+L-TYPE FAMILIES OF AMINO-ACID TRANSPORTERS 13
1.4.2 SLC3A2, HETERODIMERIC
PARTNER OF L-TYPE AND Y+L-TYPE TRANSPORTERS 14
1.4.3 SLC7A7, BETWEEN
METABOLISM AND IMMUNITY 15
2 AIM
OF THE PROJECT 18
3 RESULTS 19
3.1 ISOLATION AND CHARACTERISATION OF MUTANTS LACKING MICROGLIA 19
3.2 N067/BLUEBEARD LACKS MICROGLIA AND HAS A NORMAL MACROPHAGES AND
NEUTROPHILS DISTRIBUTION 21
3.2.1 N067/BBD MACROPAHAGES ARE MOTILE AND ARE ABLE TO MIGRATE TO SITES
OF INJURY 22
3.2.2 THE
GENE UNDERLYING THE N067/BBD PHENOTYPE IS REQUIRED FOR BOTH
DEVELOPMENTAL COLONIZATION AND
MICROGLIAL ENTRY IN RESPONSE TO ACUTE STIMULI. 24
3.3 GENETIC MAPPING AND CLONING OF N067/BBD 27
3.3.1 SLC7A7 EXPRESSION
REVEALS A NOVEL SUBPOPULATION OF MACROPHAGES 32
3.3.2 GENERATION OF AN IN VIVO REPORTER OF SLC7A7 EXPRESSION 35
3.3.3 MECHANISTIC ROLE OF SLC7A7 IN THE ESTABLISHMENT OF MICROGLIAL
PRECURSORS 39
4 DISCUSSION 44
5 MATERIALS AND METHODS 49
5.1 FISH REARING AND HANDLING 49
5.1.1 ZEBRAFISH STRAINS AND
TRANSGENIC LINES 49
5.1.2 FISH HUSBANDRY 49
5.1.3 BLEACHING
PROCEDURE 50
5.1.4 PTU
TREATMENT 51
HTTP://D-NB.INFO/104703011X
5.1.5 ANAESTHESIA
OF ZEBRAFISH EMBRYOS 51
5.1.6 LIVE IMAGING
OF ZEBRAFISH EMBRYOS 51
5.1.7 INJECTION IN ZEBRAFISH EMBRYOS 52
5.1.8 MORPHOLINOS 52
5.2 MOLECULAR BIOLOGY TECHNIQUES 52
5.2.1 TOTAL RNA EXTRACTION
FROM ZEBRAFISH EMBRYOS 52
5.2.2 DNASE
I TREATMENT 53
5.2.3 CDNA
SYNTHESIS 53
5.2.4 POLYMERASE
CHAIN REACTION (PCR) FOR SEQUENCING 54
5.2.5 CLONING OF CDNAS 57
5.2.6 SUBCLONING OF GENES FOR MRNA
PRODUCTION 57
5.2.7 COLONY PCRS 57
5.2.8 MRNA
PRODUCTION 58
5.2.9 BACS
MODIFICATION
;
59
5.2.10 GENERATION OF A
CHIMERIC HUMAN CD69 / ZEBRAFISH SLC3A2 CONSTRUCT 60
5.3 HISTOLOGICAL METHODS 61
5.3.1 NEUTRAL RED STAINING 61
5.3.2 WHOLE-MOUNT IN SITU
HYBRIDIZATION (ISH) 61
5.4 CHEMICAL TREATMENTS 62
5.5 MICROSCOPY TECHNIQUES 63
5.5.1 CONFOCAL MICROSCOPES 63
5.5.2
TAIL CUT AND
LEUKOCYTES TRACKING 63
5.5.3 MICROGLIA
ABLATION AND INDUCTION OF NEURONAL APOPTOSIS 63
5.6 SOFTWARE 64
6 BIBLIOGRAPHY 66
7 APPENDIX 79
7.1 LIST OF ABBREVIATIONS 79
7.2 COLLABORATIONS 80
|
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| author | Rossi, Federico Maria |
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| author_facet | Rossi, Federico Maria |
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| indexdate | 2024-07-10T01:04:09Z |
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| language | English |
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| spelling | Rossi, Federico Maria Verfasser (DE-588)1046464353 aut The role of slc7a7 in promoting brain colonization by microglia [presented by Federico Maria Rossi] 2013 80 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Heidelberg, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027184995&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rossi, Federico Maria The role of slc7a7 in promoting brain colonization by microglia |
| subject_GND | (DE-588)4113937-9 |
| title | The role of slc7a7 in promoting brain colonization by microglia |
| title_auth | The role of slc7a7 in promoting brain colonization by microglia |
| title_exact_search | The role of slc7a7 in promoting brain colonization by microglia |
| title_full | The role of slc7a7 in promoting brain colonization by microglia [presented by Federico Maria Rossi] |
| title_fullStr | The role of slc7a7 in promoting brain colonization by microglia [presented by Federico Maria Rossi] |
| title_full_unstemmed | The role of slc7a7 in promoting brain colonization by microglia [presented by Federico Maria Rossi] |
| title_short | The role of slc7a7 in promoting brain colonization by microglia |
| title_sort | the role of slc7a7 in promoting brain colonization by microglia |
| topic_facet | Hochschulschrift |
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