Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis: potential implications for antifibrotic therapies
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Aus: Arthritis & rheumatism ; 2012. - Enth. einen weiteren Sonderdr. |
| Beschreibung: | 50 S. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | INHALTSVERZEICHNIS
ZUSAMMENFASSUNG / SUMMARY 1
ZUSAMMENFASSUNG 1
SUMMARY 2
EINLEITUNG / INTRODUCTION 4
EINLEITUNG 4
INTRODUCTION 7
VORVEROFFENTLICHUNGEN 10
HEDGEHOG SIGNALING CONTROLS FIBROBLAST ACTIVATION AND
TISSUE FIBROSIS IN SYSTEMIC
SCLEROSIS 10
ABSTRACT 12
INTRODUCTION 13
METHODS 15
PATIENTS AND FIBROBLAST CULTURES 15
IMMUNOHISTOCHEMISTRY FOR SHH, GLI-1, GLI-2,
GLI-3 AND A-SMASTIMULATION WITH
SHH 15
STIMULATION WITH SHH 16
QUANTITATIVE REAL-TIME PCR 16
COLLAGEN MEASUREMENTS 17
MRNA-STABILITY ASSAY 17
LUCIFERASE REPORTER ASSAY 17
IMMUNOFLUORESCENCE FOR A-SMA 18
STRESS FIBER STAINING 18
ADENOVIRAL OVEREXPRESSION OF SHH 18
BLEOMYCIN INDUCED DERMAL FIBROSIS IN PTCH
+/
MICE 19
HISTOLOGICAL ANALYSIS 19
ACTIVATION OF TGF-P- AND CANONICAL
WNT SIGNALING IN THE SKIN OF MICE 19
STATISTICS 20
RESULTS 21
THE HEDGEHOG PATHWAY IS ACTIVATED IN SSC
PATIENTS 21
TGF-P- AND CANONICAL WNT SIGNALING
INDUCE SSC 21
SHH STIMULATES THE RELEASE OF EXTRACELLULAR MATRIX AND INDUCES FIBROSIS
22
SHH-INDUCED RELEASE OF EXTRACELLULAR MATRIX IS MEDIATED
BY ENHANCED
TRANSCRIPTION OF COL
1A1 AND COL 1A2 23
SHH INDUCES MYOFIBROBLASTS DIFFERENTIATION 23
ADENOVIRAL OVEREXPRESSION OF SHH INDUCES DERMAL FIBROSIS
IN MICE 24
PTCH
+/
MICE ARE MORE SENSITIVE TO BLEOMYCIN INDUCED SKIN FIBROSIS 25
DISCUSSION 26
ACKNOWLEDGEMENTS 28
REFERENCES 29
FIGURE LEGENDS 31
FIGURES 33
INHIBITION OF HEDGEHOG SIGNALING PREVENTS EXPERIMENTAL FIBROSIS
AND INDUCES
REGRESSION OF ESTABLISHED FIBROSIS 39
ABSTRACT 39
INTRODUCTION : 39
METHODS 39
INHIBITION OF SMO BY LDE223 39
IN VIVO TRANSFECTION
WITH SIRNAS AGAINST SMO IN BLEOMYCIN-INDUCED SKIN
FIBROSIS 40
HISTOLOGICAL ANALYSIS 40
IMMUNOHISTOCHEMISTRY FOR GLI-1, GLI-2 AND A-SMOOTH MUSCLE ACTIN 40
HYDROXYPROLINE ASSAY 40
QUANTITATIVE REAL-TIME PCR 42
STATISTICS 42
RESULTS 42
HTTP://D-NB.INFO/1045622699
INHIBITION OF SMO ABROGATES THE ABERRANT ACTIVATION OF HEDGEHOG
SIGNALING IN
EXPERIMENTAL MODELS OF DERMAL FIBROSIS 42
INHIBITION OF SMO BY LDE223 PREVENTS BLEOMYCIN-INDUCED DERMAL FIBROSIS
....42
SIRNA-MEDIATED KNOCKDOWN OF SMO PREVENTS EXPERIMENTAL
FIBROSIS 43
INHIBITION OF SMO CORRECTS THE TSK-1 PHENOTYPE 43
DISCUSSION 43
REFERENCES 43
SUPPLEMENTARY DATA 44
LITERATURVERZEICHN
IS 45
ABKIIRZUNGSVERZEICHNIS 49
DANKSAGUNG 50
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| spelling | Horn, Angelika Verfasser aut Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies vorgelegt von Angelika Horn 2013 50 S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Aus: Arthritis & rheumatism ; 2012. - Enth. einen weiteren Sonderdr. Erlangen-Nürnberg, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027178237&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Horn, Angelika Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies |
| subject_GND | (DE-588)4113937-9 |
| title | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies |
| title_auth | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies |
| title_exact_search | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies |
| title_full | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies vorgelegt von Angelika Horn |
| title_fullStr | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies vorgelegt von Angelika Horn |
| title_full_unstemmed | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies vorgelegt von Angelika Horn |
| title_short | Hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis |
| title_sort | hedgehog signaling controls fibroblast activation and tissue fibrosis in systemic sclerosis potential implications for antifibrotic therapies |
| title_sub | potential implications for antifibrotic therapies |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027178237&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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